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Anti rac

Manufactured by Cell Signaling Technology
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Anti-Rac is a primary antibody that binds to the Rac protein, a member of the Rho family of small GTPases. Rac plays a crucial role in regulating cell signaling pathways involved in cell migration, cytoskeletal organization, and other cellular processes. Anti-Rac is a valuable tool for researchers studying Rac-mediated cellular functions.

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Lab products found in correlation

Anti cdc42, by Abcam (2 mentions) β actin, by Merck Group (2 mentions) Anti pak1 pak2 pak3, by Cell Signaling Technology (2 mentions) Anti ppak1 t423 pak2 t402, by Abcam (2 mentions) Anti pak1 pak2 pak3 phospho t423, by Abcam (2 mentions) Anti integrin β3, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Anti cd68, by BioLegend (1 mentions) Anti p src, by Cell Signaling Technology (1 mentions) Anti integrin β1, by Cell Signaling Technology (1 mentions) Lactate dehydrogenase ldh assay, by Beyotime (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Pf 573228, by Selleck Chemicals (1 mentions) Anti fak, by Cell Signaling Technology (1 mentions) Anti src, by Cell Signaling Technology (1 mentions) Pf 431396, by Merck Group (1 mentions) Anti pfak397, by Cell Signaling Technology (1 mentions) Anti cse, by Santa Cruz Biotechnology (1 mentions) Anti p pyk2, by Cell Signaling Technology (1 mentions) Nsc23766, by Selleck Chemicals (1 mentions)

3 protocols using anti rac

1

Western Blot Analysis of Rho GTPases

Cited 1 time
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Total cell lysates or pulldowns were Western blotted using routine procedures. The primary antibodies used were anti-Rac (Cell Signaling Technology), anti-Cdc42 (Abcam), anti-PAK1+PAK2+PAK3 (Cell Signaling Technology), anti-pPAK1 (T423)/PAK2 (T402; Abcam), anti-PAK1 + PAK2 + PAK3 (phospho T423; Abcam), Rac1B, and β-actin (Millipore-Sigma). Actin was used as a control; the Rac/Cdc42 inhibitors do not affect total actin expression, only its polymerization and organization of the cytoskeleton (23 (link)).
Medina J.I., Cruz-Collazo A., Maldonado M.D., Matos Gascot T., Borrero-Garcia L.D., Cooke M., Kazanietz M.G., Hernandez O'Farril E., Vlaar C.P, & Dharmawardhane S. (2022). Characterization of Novel Derivatives of MBQ-167, an Inhibitor of the GTP-binding Proteins Rac/Cdc42. Cancer Research Communications, 2(12), 1711-1726.
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2

Inhibition of Pyk2 and Rac1 Signaling

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NaHS was administered instead of H2S. NaHS and the Pyk2 inhibitor (PF431396) were obtained from Sigma-Aldrich; The FAK specific inhibitor (PF573228), the Src inhibitor (PP2), and the Rac1 inhibitor (NSC23766) were purchased from Selleck Chemicals. CES-siRNA (sc-142618) was obtained from Santa Cruz. Rhodamine was obtain from Cytoskeleton, and DAPI from Beyotime; Following antibodies were used: anti-Rac, anti-Fak, anti-p-FAK397, anti-p-FAK925, anti-Src, anti-p-Src, anti-β-actin, anti-Integrin β1, anti-Integrin β3, anti-Cavenolin-1,anti-p-Pyk2 (Cell Signaling); anti-CSE (Santa Cruz); anti-Integrin β1-FITC, anti-Galectin-3 (eBbioscience); anti-CD68 (Biolegend); Lactate dehydrogenase (LDH) assay from BeyotimeInstitute of Biotechnology.
Miao L., Xin X., Xin H., Shen X, & Zhu Y.Z. (2016). Hydrogen Sulfide Recruits Macrophage Migration by Integrin β1-Src-FAK/Pyk2-Rac Pathway in Myocardial Infarction. Scientific Reports, 6, 22363.
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3

Immunoblotting of Rho GTPases

Cited 1 time
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Total cell lysates or pull-downs were Western blotted using routine procedures. The primary antibodies used were anti-Rac (Cell Signaling), anti-Cdc42 (Abcam), anti-PAK1+PAK2+PAK3 (Cell Signaling), anti-pPAK1 (T423)/PAK2 (T402) (Abcam), Anti-PAK1 + PAK2 + PAK3 (phospho T423) (Abcam), and β -actin (Millipore-Sigma). Actin was used as a control; the Rac/Cdc42 inhibitors do not affect total actin expression, only its polymerization and organization of the cytoskeleton (23 (link)).
Medina J.I., Cruz-Collazo A., Maldonado M.D., Matos Gascot T., Borrero-Garcia L.D., Cooke M., Kazanietz M.G., Hernandez O'Farril E., Vlaar C.P, & Dharmawardhane S. (2022). Characterization of Novel Derivatives of MBQ-167, an Inhibitor of the GTP-binding Proteins Rac/Cdc42. Cancer Research Communications, 2(12), 1711-1726.
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