Thp 1 cell line
The THP-1 cell line is a human monocytic cell line derived from an acute monocytic leukemia patient. It is a widely used in vitro model for the study of monocyte and macrophage biology. The THP-1 cell line can differentiate into macrophage-like cells in response to various stimuli.
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6 protocols using thp 1 cell line
Cell Line Cultivation and Polarization Protocols
Evaluating Mg Antioxidant Capacity
All different assays were performed under basal and stimulating pro-inflammatory conditions, such as those induced by bacterial lipopolysaccharide (LPS), to assess whether the antioxidant capacity of Mg could protect patients from potential high-risk situations.
Generating M0, M1 and M2 Macrophages from THP-1 Cells
Culturing Human Monocytic Leukemia Cells
Monocyte Polarization Protocol
THP-1 cell line (Sigma–Aldrich, St-Louis, MO, USA), a human monocytic cell line derived from monocytic leukemia patients, was cultured in RPMI 1640 supplemented with L-glutamine, 10% fetal calf serum (FCS; VWR International Ltd, Lutterworth, UK) and 1% penicillin and streptomycin (PenStrep) at 37 °C with 5% CO2.
THP-1 or monocytes were seeded into 24-well plates (6 × 105cells/mL) in RPMI 1640 and were left untreated or were treated with 20 ng/mL interleukin-4 (IL-4; Fischer Scientific, Loughborough, UK), 20 ng/mL interleukin-10 (IL-10; PeproTech, London, UK) or 100 ng/mL lipopolysaccharide (LPS; Sigma–Aldrich) for 24 h or in some experiments for the time of 48 h. Cells were washed following stimulation to ensure serum was removed.
Cell Culture Protocol for THP-1, BICR-18, and CAPAN-2
The human epithelial cell line BICR-18 (from larynx squamous cell carcinoma) and CAPAN-2 (from pancreatic ductal adenocarcinoma) were obtained from American Type Cell Collection (ATCC, Manassas, VA, USA) and maintained at 37 °C in DMEM GlutaMAX™ (Fisher Scientific; Madrid, Spain) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cultures were split every 3 days by trypsinization with 0.1% trypsin in Ca2+/Mg2+-free phosphate-buffered saline (PBS) containing 0.9 mM ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich; St. Louis, MO, USA).
All cells were cultured at 37 °C in humidified atmosphere of 95% air and 5% CO2.
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