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Functional grade anti cd40

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Functional-grade anti-CD40 is a purified monoclonal antibody that specifically binds to the CD40 receptor on the surface of B cells and antigen-presenting cells. It can be used for research purposes to study immune cell function and activation.

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Lab products found in correlation

Lipopolysaccharide lps, by Merck Group (2 mentions) Hepes ph 7, by Thermo Fisher Scientific (2 mentions) 2 β mercaptoethanol, by Thermo Fisher Scientific (2 mentions) Interleukin 4 (il 4), by R&D Systems (2 mentions) Anti cd43 microbeads, by Miltenyi Biotec (2 mentions) Lipopolysaccharides (lps), by Miltenyi Biotec (2 mentions)

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Anti-CD40 (70 citations)

2 protocols using functional grade anti cd40

1

Primary B Cell Activation Protocol

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For LPS-IL-4-anti-CD40 cultures, primary B cells were purified by negative selection using anti-CD43 MicroBeads (Miltenyi Biotec) and cultured in complete RPMI supplemented with 10% FBS, 50 mM of 2-β-Mercaptoethanol (Gibco), 20 mM HEPES pH 7.2–7.5 (Gibco), 25 ng/ml of IL-4 (R&D), 25 μg/ml lipopolysaccharide (LPS, Sigma-Aldrich) and 1 μg/ml functional-grade anti-CD40 (ThermoFisher Scientific).
Rivas M.A., Meydan C., Chin C.R., Challman M.F., Kim D., Bhinder B., Kloetgen A., Viny A.D., Teater M.R., McNally D.R., Doane A.S., Béguelin W., Fernández M.T., Shen H., Wang X., Levine R.L., Chen Z., Tsirigos A., Elemento O., Mason C.E, & Melnick A.M. (2021). Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation. Nature immunology, 22(2), 240-253.
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2

Primary B Cell Activation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For LPS-IL-4-anti-CD40 cultures, primary B cells were purified by negative selection using anti-CD43 MicroBeads (Miltenyi Biotec) and cultured in complete RPMI supplemented with 10% FBS, 50 mM of 2-β-Mercaptoethanol (Gibco), 20 mM HEPES pH 7.2–7.5 (Gibco), 25 ng/ml of IL-4 (R&D), 25 μg/ml lipopolysaccharide (LPS, Sigma-Aldrich) and 1 μg/ml functional-grade anti-CD40 (ThermoFisher Scientific).
Rivas M.A., Meydan C., Chin C.R., Challman M.F., Kim D., Bhinder B., Kloetgen A., Viny A.D., Teater M.R., McNally D.R., Doane A.S., Béguelin W., Fernández M.T., Shen H., Wang X., Levine R.L., Chen Z., Tsirigos A., Elemento O., Mason C.E, & Melnick A.M. (2021). Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation. Nature immunology, 22(2), 240-253.
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