4 6 diamidino 2 phenylindole dapi

Manufactured by Nichirei Biosciences

Sourced in Japan

4',6-diamidino-2-phenylindole (DAPI) is a fluorescent dye used for the detection and quantification of DNA in biological samples. It binds strongly to adenine-thymine (A-T) rich regions of double-stranded DNA, emitting a blue fluorescence when excited by ultraviolet (UV) light.

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Lab products found in correlation

Anti myc, by Medical & Biological Laboratories (2 mentions) Anti gfp, by Medical & Biological Laboratories (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Fv1000 confocal laser microscope, by Olympus (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 and 568, by Thermo Fisher Scientific (1 mentions) Anti neun, by Merck Group (1 mentions)

3 protocols using 4 6 diamidino 2 phenylindole dapi

Isolation and Culture of Mouse Hippocampal Neurons

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Mouse primary hippocampal neurons were isolated as follows. Briefly, hippocampal tissue was isolated from embryonic day 16 (E16) mouse pups. Then, gentle dissociation of neuronal cells was performed with trypsin (0.25%) and mechanical disruption was done to separate cells from connective tissue while providing minimum damage to individual neuronal cells [19 ]. The isolated neurons were cultured essentially as described [3 (link), 6 (link)]. Transient transfection was carried out using Lipofectamine 2000 (Life Technologies). Immunofluorescence analysis was done as described [6 (link)]. Briefly, cells grown on 13-mm coverslips were fixed in 3.7% formaldehyde in PBS for 15 min, then treated with 0.2% Triton-X 100 for 5 min. 4',6-diamidino-2-phenylindole (DAPI; Nichirei Bioscience, Tokyo, Japan) and anti-ARHGEF9 were used for staining of DNA and ARHGEF9, respectively. Alexa Fluor 488-labeled IgG (Life Technologies) was used as a secondary antibody. Images were captured using FV-1000 confocal laser microscope (Olympus, Tokyo, Japan).

Ibaraki K., Mizuno M., Aoki H., Niwa A., Iwamoto I., Hara A., Tabata H., Ito H, & Nagata K.I. (2018). Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues. Acta Histochemica et Cytochemica, 51(3), 119-128.

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Immunofluorescence Staining of GFP, Myc, and Phospho-PAK1

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The following antibodies were used: anti-GFP (Medical & Biological Laboratories, Nagoya, Japan, Cat# 598, RRID: AB_591819 or Nacalai Tesque, Kyoto, Japan, Cat# 04404-84, RRID: AB_10013361), anti-Myc (Medical & Biological Laboratories, Nagoya, Japan, Cat# M047-3, RRID: AB_591112), and anti-phospho-PAK1 (Thr423) (Cell Signaling Technology Cat#2601, RRID: AB_330220). Alexa Fluor 488 (Invitrogen, Carlsbad, CA, USA) was used as a secondary antibody. 4′, 6-diamidino-2-phenylindole (DAPI; Nichirei Bioscience, Tokyo, Japan) was used for staining DNA.

Nishikawa M., Ito H., Tabata H., Ueda H, & Nagata K.I. (2022). Impaired Function of PLEKHG2, a Rho-Guanine Nucleotide-Exchange Factor, Disrupts Corticogenesis in Neurodevelopmental Phenotypes. Cells, 11(4), 696.

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Multicolor Immunofluorescence Staining

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The following antibodies were used: anti-GFP (Medical and Biological Laboratories Cat #598, RRID: AB_591819 or Nacalai Tesque, Cat #04404-84, RRID: AB_10013361), anti-NeuN (Millipore, Cat #MAB377, RRID:AB_2298772) and anti-Myc (Medical and Biological Laboratories, Cat #M047-3, RRID: AB_591112). Alexa Fluor 488 and 568 (Invitrogen) were used as secondary antibodies. 4′,6-diamidino-2-phenylindole (DAPI; Nichirei Bioscience) was used for staining DNA. Rhodamine-phalloidin (Invitrogen) was used for staining filamentous actin.

Scala M., Nishikawa M., Ito H., Tabata H., Khan T., Accogli A., Davids L., Ruiz A., Chiurazzi P., Cericola G., Schulte B., Monaghan K.G., Begtrup A., Torella A., Pinelli M., Denommé-Pichon A.S., Vitobello A., Racine C., Mancardi M.M., Kiss C., Guerin A., Wu W., Gabau Vila E., Mak B.C., Martinez-Agosto J.A., Gorin M.B., Duz B., Bayram Y., Carvalho C.M., Vengoechea J.E., Chitayat D., Tan T.Y., Callewaert B., Kruse B., Bird L.M., Faivre L., Zollino M., Biskup S., Striano P., Nigro V., Severino M., Capra V., Costain G, & Nagata K.I. (2022). Variant-specific changes in RAC3 function disrupt corticogenesis in neurodevelopmental phenotypes. Brain, 145(9), 3308-3327.

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