Au640 automatic blood biochemical analyzer

The blood cell composition of anticoagulant blood collected in EDTA tubes was detected using a fully automated blood cell analyzer (Sysme XT-1800i, Shanghai, China). An Olympus AU640 automatic blood biochemical analyzer was used to detect the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The preserved serum was collected using a commercial kit (Shanghai Enzyme-linked ImmunoBiotechnology Co., Ltd., Shanghai, China) and an enzyme labeler (MultiskanM™ SkyHigh, Thermo Fisher Scientific, Waltham, MA, USA) for antioxidant and immune markers. Levels of malondialdehyde (MDA), reactive oxygen species (ROS), glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were used as serum antioxidant indexes. Levels of tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6), and interleukin-10 (IL-10) were taken as serum immune indicators.

The blood cell components in the anticoagulant blood collected from the EDTA tube were detected using an automatic hematology analyzer (SysmeXT-1800i, Shanghai, China). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using the Japanese Olympus AU640 automatic blood biochemical analyzer. We used commercial kits (Shanghai Enzyme Linked Immunobiotechnology Co. Ltd., Shanghai, China) and an automated fluorescence instrument (MultiskanM™ SkyHigh, Thermo Fisher Scientific, Waltham, MA, USA) to detect antioxidant and immune indicators in the preserved serum. Serum markers of oxidative stress included malondialdehyde (MDA), and reactive oxygen species (ROS). Serum antioxidant indicators included glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC). Serum immune indicators included tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), interleukin-4 (IL-4), interleukin-6 (IL-6), and interleukin-10 (IL-10).