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2 protocols using anti β actin antibody ac026

1

Neuroinflammation Pathway Modulation

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Lithium chloride, pilocarpine, polyethylene glycol, lipopolysaccharide (LPS), ATP, 2,3-O-4-benzoyl-4-benzoyl-ATP (BzATP) and oxidized ATP (oxATP) were purchased from Sigma (USA). Diazepam was obtained from Yimin (Beijing, China), AST from Solarbio (Beijing, China), dimethyl sulfoxide (DMSO) from Amresco (Beijing, China), fetal bovine serum (FBS), Dulbecco’s modified eagle medium (DMEM) high glucose medium from Gibco (USA), and the trizol reagent and RT-PCR kits from Takara (Shanghai, China). P2X7R polyclonal antibody#A10511, TNF-α polyclonal antibody#A11534, IL-1β polyclonal antobody#A16288, Iba1 polyclonal antibody, and anti-β-actin antibody#AC026 were purchased from ABclonal (Wuhan, China). Cox-2 (D5H5) ZP@ Rabbit mAb#12282 was obtained from Cell Signaling Technology (USA). HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) #SA00001-2 was obtained from Proteintech (Shanghai, China). ATP enzyme-linked immunosorbent assay (ELISA) kits were obtained from Halin company (Shanghai, China), and Nissl stain kits and immunohistochemical stain kits were obtained from Ruibo company (Shanghai, China).
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2

Monoclonal Antibodies for Apoptosis Signaling

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Mouse monoclonal antibodies against AQP1 (ab9566) and RIPK1 (ab72139) were purchased from Abcam (Cambridge, MA). Mouse monoclonal antibodies against RIPK1 (610458), caspase-8 (9746), and RIPK3 (sc-374639) were bought from BD Biosciences (San Jose, CA), Cell Signaling Technology (Danvers, MA), and Santa Cruz Biotechnology (Dallas, TX), respectively. The rabbit monoclonal antibody against p-MLKL (S345) (ab196436), p-RIPK3 (S227) (ab209384) and p-RIPK3 (S232) (ab195117), and rabbit polyclonal antibody against RIPK1 (ab106393) and MLKL (ab194699) were purchased from Abcam. The rabbit polyclonal antibody against cleaved caspase-3 (9661) and caspase-3 (9662) were bought from Cell Signaling Technology. The rabbit monoclonal anti-β-actin antibody (AC026) and anti-α-tubulin antibody (AC013) were from Abclonal (Wuhan, China). The cell transfection regent (F231-02) was purchased from TransGen Biotech (Beijing, China). The point mutation plasmids of RIPK1 were constructed using the HiFi HotStart DNA Polymerase (KAPA Biosystems, Roche, Switzerland).
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