Rp 8 br cgmps

Primary antibodies of cGMP-dependent protein kinase 1 (cGK I), cGK II, proliferating cell nuclear antigen (PCNA), MAPK phosphatase-1 (MKP-1), p-Erk1/2, p-p38, p21^Cip1, and p27^Kip1 were obtained from Santa Cruz Biotechnology (San Diego, CA, USA). Anti-mouse secondary antibody conjugated with Alexa Fluor® 647, 488 and ProLong Gold antifade reagent with 4’, 6-diamidino-2-phenylindole (DAPI) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). A71915 and Rp-8-Br-cGMPS (hereafter indicated as Rp) were purchased, respectively, from Bachem (Torrance, CA, USA) and EMD Calbiochem (San Diego, CA, USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). A creatinine kit was purchased from BioAssay System (Hayward, CA, USA). A microalbumin assay kit was purchased from Bethyl Laboratories (Montgomery, TX, USA). RNase-free DNase was obtained from Qiagen (Valencia, CA, USA). A multiplex kit for mouse cytokine assay was purchased from Millipore (Billerica, MA, USA). A cGK activity assay kit was purchased from MBL International (Woburn, MA, USA). A cGMP assay kit was purchased from Assay Designs (Ann Arbor, MI, USA). All other chemicals were of reagent grade.