Isotype rat igg2baccording

Manufactured by BD

Isotype rat IgG2b is a type of antibody used in research applications. It serves as a control to establish baseline responses in experiments involving rat immunoglobulin G2b antibodies.

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Lab products found in correlation

Anti cd25 pe, by BD (2 mentions) Pe cy5 anti cd4, by BD (2 mentions) Anti foxp3 alexa fluor 488, by BD (2 mentions) Mouse foxp3 buffer set, by BD (2 mentions)

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Isotypes (2 citations)

2 protocols using isotype rat igg2baccording

Regulatory T Cell Phenotyping by Flow Cytometry

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Freshly isolated splenocytes were stained with PE-Cy5 anti-CD4 and PE-anti-CD25 or isotypes rat IgG2a,κ and rat IgM,κ, respectively, for 10 min at 4 °C (BD). Cells were then fixed and permeabilized using the Mouse FoxP3 Buffer Set and then incubated with Alexa Fluor 488 anti-FoxP3 or isotype rat IgG2baccording to manufacturer’s instructions (BD). Flow cytometry was performed immediately or within 24 h of permeabilization using a FACScaliber machine. 1 × 105 gated events were recorded. Results were analyzed with FCS Express software.

Garg S.K., Delaney C., Toubai T., Ghosh A., Reddy P., Banerjee R, & Yung R. (2014). Aging is associated with increased regulatory T-cell function. Aging Cell, 13(3), 441-448.

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Murine Regulatory T Cell Phenotyping

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Freshly isolated splenocytes were stained with PE-Cy5 anti-CD4 and PE anti-CD25 or isotypes rat IgG2a,κ and rat IgM,κ, respectively, for 10 min at 4°C (BD). Cells were then fixed and permeabilized using the Mouse FoxP3 Buffer Set, then incubated with Alexa Fluor 488 anti-FoxP3 or isotype rat IgG2baccording to manufacturer’s instructions (BD). Flow cytometry was performed immediately or within 24 h of permeabilization using a FACScaliber machine. 1 × 105 gated events were recorded. Results were analyzed with FCS Express software.

Garg S.K., Delaney C., Toubai T., Ghosh A., Reddy P., Banerjee R, & Yung R. (2014). Aging is associated with increased regulatory T-cell function. Aging Cell, 13(3), 441-448.

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