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Macrophage colony stimulating factor

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Macrophage colony-stimulating factor is a protein that stimulates the production and differentiation of macrophages, a type of white blood cell. It plays a crucial role in the immune system by regulating the growth and maturation of these cells.

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Lab products found in correlation

Recombinant human ifn γ, by Thermo Fisher Scientific (1 mentions) Recombinant human il 4, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Linoleic acid, by Merck Group (1 mentions) Fibroblast growth factor 4, by Merck Group (1 mentions) β mercaptoethanol, by Merck Group (1 mentions)

4 protocols using macrophage colony stimulating factor

1

Macrophage Activation Protocol

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Recombinant human IL-4 and recombinant human IFN-γ were purchased from Life Technologies (Grand Island, NY, USA), LPS was purchased from Sigma-Aldrich (St Louis, MO, USA), and macrophage colony-stimulating factor was obtained from Prospec (Rehovot, Israel).
Cho D.I., Kim M.R., Jeong H.Y., Jeong H.C., Jeong M.H., Yoon S.H., Kim Y.S, & Ahn Y. (2014). Mesenchymal stem cells reciprocally regulate the M1/M2 balance in mouse bone marrow-derived macrophages. Experimental & Molecular Medicine, 46(1), e70-.
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2

Differentiation of Macrophages from Monocytes

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Human elutriated monocytes were resuspended at 1.8×105 cells/ml in RPMI medium containing 10% FBS and macrophage colony-stimulating factor (20 ng/ml, ProSpec, Israel), plated at 0.5 ml/well on eight-chamber Lab-Tek tissue-culture slides (Miles Laboratories) and incubated for 9 days for differentiation into macrophages. The macrophage infection experiments were performed essentially as described earlier [19] (link).
Gannavaram S., Davey S., Lakhal-Naouar I., Duncan R, & Nakhasi H.L. (2014). Deletion of Ubiquitin Fold Modifier Protein Ufm1 Processing Peptidase Ufsp in L. donovani Abolishes Ufm1 Processing and Alters Pathogenesis. PLoS Neglected Tropical Diseases, 8(2), e2707.
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3

Monocyte Differentiation to NeoHep

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The differentiation of monocytes to NeoHep was achieved by using a two‐step differentiation protocol. In the first step, monocytes were differentiated to “reprogrammed monocytes” (RM) in the presence of basal Iscove's modified Dulbecco's medium (IMDM; Thermo Fisher Scientific, Waltham, MA,
https://www.thermofisher.com), supplemented with interleukin‐3, macrophage colony‐stimulating factor (Prospec, Ness‐Ziona, Israel,
http://www.prospecbio.com), β‐mercaptoethanol (Sigma, St. Louis, MO,
https://www.sigmaaldrich.com), and 0.5% embryonic stem cell‐grade fetal bovine serum (eFBS) (Biological Industries, Kibbutz Beit‐Haemek, Israel,
http://www.bioind.com). After day 6, RM were differentiated to NeoHep in 15 days in the presence of IMDM, supplemented with epithelial growth factor (Prospec), hepatocyte growth factor (HGF), fibroblast growth factor‐4, linoleic acid (Sigma), and eFBS. Detailed steps are given in the
supplemental online data.
Bhattacharjee J., Das B., Sharma D., Sahay P., Jain K., Mishra A., Iyer S., Nagpal P., Scaria V., Nagarajan P., Khanduri P., Mukhopadhyay A, & Upadhyay P. (2016). Autologous NeoHep Derived from Chronic Hepatitis B Virus Patients’ Blood Monocytes by Upregulation of c‐MET Signaling. Stem Cells Translational Medicine, 6(1), 174-186.
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4

Murine Macrophage Differentiation and Stimulation

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Bone marrow cells were collected from the femurs and tibias of Balb/c mice aged 6 to 8 wk old. Bone marrow cells were seeded at 1 × 10 6 cells/cm 2 into 24well plates and grown for 7 d in αMEM supplemented with 10% fetal bovine serum, antibiotics (all from Invitrogen, Grand Island, NY) and 30 ng/mL macrophage colony stimulating factor (Prospec, Ness-Ziona, Israel) under standard conditions at 37°C, 5% CO 2 , and 95% humidity. Primary macrophages were exposed to 1% formula for 1 h before being exposed to 5% saliva for 24 h based on our established protocol (Pourgonabadi et al., 2017) (link).
Panahipour L., Tabatabaei A.A, & Gruber R. (2020). Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula. Journal of dairy science, 103(8).
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