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Sodium carbonate

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Sodium carbonate is a water-soluble inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used as a pH regulator, water softener, and cleaning agent in various industrial and laboratory applications.

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1 294 protocols using sodium carbonate

1

Antioxidant and Cytotoxicity Assays

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1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), ferrous sulfate (FeSO4.7H2O), aluminium chloride (AlCl3), sodium acetate (C2H3NaO2), sodium carbonate (Na2CO3), potassium persulfate (K2S2O8), potassium chloride (KCl), ferric chloride (FeCl3), sodium nitroprusside {Na2[Fe(CN)5NO].2H2O}, sulfanilic acid (C6H7NO3S), butylated hydroxytoluene (BHT), ascorbic acid, gallic acid, quercetin and catechin were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI medium 1640, penicillin, streptomycin, trypsin-EDTA, sulphorhodamine B, trichloroacetic acid, acetic acid, Tris base and all other chemicals used for the cytotoxicity assay were of analytical grade and also purchased from Sigma-Aldrich (St. Louis, MO, USA). Folin Ciocalteu's phenol reagent, vanillin, silica gel precoated aluminium TLC plates (20×20 cm), hydrochloric acid, sulphuric acid, methanol, n-hexane, chloroform, ethanol, toluene, dioxane, acetic acid, diethyl ether, formic acid and sodium carbonate were purchased from Merck Chemical Supplies (Merck KGaA, Darmstadt, Germany). All the other chemicals used including solvents were of analytical grade.
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2

Antioxidant Capacity Evaluation Protocol

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Folin-Ciocalteu reagent, sodium carbonate, gallic acid, sodium nitrite (NaNO2), aluminum chloride hexahydrate (AlCl3·6H2O), (+)-catechin, sodium carbonate (Na2CO3), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis(3-ethylbenothiazoline-6-sulphonic acid) diammonium salt (ABTS), potassium persulfate, and Trolox were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). High-performance liquid chromatography-grade water, acetonitrile, and methanol were purchased from J.T. Baker Inc. (Phillipsburg, NJ, USA). Reagents were the highest grade quality.
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3

Antioxidant Activity and Phytochemical Analysis

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Sodium tungstate, sodium molybdate, phosphoric acid, lithium sulfate, sodium carbonate (Na2CO3), gallic acid, sodium nitrite (NaNO2), aluminum chloride (AlCl3), sodium hydroxide (NaOH), sodium carbonate (Na2CO3), 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, ferric chloride (FeCl3), and all HPLC standard markers (vanillic acid, o-coumaric acid, ferulic acid, ellagic acid, naringin, hesperidin, apigenin, cinnamic acid, resveratrol, rosmarinic acid, rutin, chlorogenic acid, gallic acid, quercetin, and kaempferol) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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4

Silk Extraction from Bombyx mori

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Bombyx mori silkworms and cocoons were sourced from Italy and Kenya, respectively. Lithium bromide was acquired from Honeywell Fluka. Ammonia and PEG (Mw = 8000 g mol−1) were obtained from Fisher Scientific. Ammonium acetate, sodium carbonate, SYPRO orange dye and sodium carbonate were purchased from Sigma-Aldrich.
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5

Phytochemical Extraction and Analysis

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All the chemicals, reagents and solvents used were analytical grade: butanol, acetic acid, distillated water and ethanol for extraction (as bioethanol 70%).
Formic acid, gallic acid as well as the thin layer chromatography (TLC) plates (60 F254 silica gel plate on aluminum support) were purchased from Merck (Darmstadt, Germany).
Methanol (HPLC-MS grade), both flavonoid standards (quercetin and gallic acid), diphenyl-1-picrylhydrazyl (DPPH), Neu (diphenylboryloxyethylamine), Aluminium chlorid AlCl 3 2%, Sodium carbonate, Trolox from and Sodium carbonate were provided by Sigma-Aldrich (Steinheim, Germany). Folin-Ciocalteu Reagent was purchased from Fluka Analytical.
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6

Characterization of Polyphenol Compounds

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Acetonitrile, sodium carbonate, hydrochloric acid, formic acid, ascorbic acid, gallic acid, Folin-Ciocalteu reagent, catechin, malvidine-3-O-glucoside, trans-piceid, trans-resveratrol, sodium phosphate dibasic dodecahydrate, potassium phosphate monobasic, sodium carbonate and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Sodium acetate and trifluoroacetic acid were obtained from Carlo Erba Reagents (Peypin, France), and methanol and phloroglucinol from Biosolve Chimie (Dieuze, France). The solvents used were High Performance Liquid Chromatography (HPLC) grade. Deionized water was obtained from a Milli-Q Advantage A10 purification system from Millipore (Fontenay sous Bois, France). Alpha-glucosidase from Saccharomyces cerevisiae, acarbose and para-nitrophenyl alpha-D-glucopyranoside (p-NPG) were purchased from Analytic Lab (Castelnau-le-Lez, France). Trans-astringin and trans-piceatannol were obtained from Carbosynth (Compton, UK) and ChromaDex (Irvine, CA, USA), respectively. Hopeaphenol, isohopeaphenol, Ɛ-viniferin, δ-viniferin and ω-viniferin were isolated from a grapevine raw shoot. The cis-isomers were obtained using Ultraviolet-C (UV-C) irradiation (254 nm) from trans-isomers.
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7

Antioxidant and Enzyme Inhibition Assays

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The liquid chromatography standards: ferulic acid and catechin were purchased from Sigma-Aldrich (Seoul, Korea). The α-glucosidase enzymes derived from Saccharomyces cerevisiae, pancreatic lipase, 4-Methylumbelliferone (4-MU), aminoguanidine (AG), Sulphuric acid, vanillin, soyasaponin B, orlistat, and acarbose were purchased from Sigma-Aldrich (Seoul, Korea). Sodium carbonate, methyl cellosolve, potassium phosphate dibasic, potassium phosphate monobasic, sodium dihydrogen phosphate, sodium phosphate dibasic sodium chloride, sodium citrate, 4-nitrophenyl α-d-glucopyranoside, Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-hydroxy2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), ethanol, sodium hydroxide, and hydrochloric acid, Sodium carbonate, and potassium per-sulfate were purchased from Sigma-Aldrich (Seoul, Korea). All the chemical reagents used were of analytical grade.
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8

Comprehensive Oxidative Stress Analysis

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Thiobarbituric acid (TBA), sodium dodecyl sulfate (SDS), glacial acetic acid, N-butanol, pyridine, 1,3,3-tetraethoxypropane (TEP), cytochrome C, xanthine oxidase, xanthine, glutathione reductase, nicotinamide adenine dinucleotide phosphate (NADPH), hydrogen peroxide, superoxide dismutase, glutathione peroxidase, catalase, acetylthiocholine iodide (ATCI), acetylcholinesterase, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), cresyl violet, sodium acetate, sodium carbonate, 2,4,6-tripyridyl-striazine (TPTZ), Folin-Ciocalteu reagent, gallic acid, ascorbic acid, Trizma hydrochloride, potassium chloride, 2,2-diphenyl-1-picrylhydrazyl (DPPH), tris-hydrochloride, and sodium carbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemicals used in Western blot analysis were purchased from Bio-Rad Laboratories. Methanol and acetic acid (HPLC grade) were purchased from Fisher Scientific.
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9

Quantification of Cyclamic Acid in Bleach

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Methanol (> 99%), n-heptane (> 99%) and 2-propanol (> 99.8%), from Merck (Germany) and sodium carbonate (> 99.8%) from, were of HPLC grade. All other reagents were of recognised analytical grade unless stated otherwise. Sodium sulphate (> 99%) and sulphuric acid (96%) was purchased from Merck (Germany) and sodium carbonate (> 99.8%) from Riedel-de-Haën (Germany). Ultra-pure type I water, generated by a Milli-Q system, was used. The calcium cyclamate standard (> 99%) was purchased from Sigma-Aldrich (USA). sodium carbonate (5%), sulphuric acid (50%) solutions and cyclamic acid standard stock solution (2 mg l -1 ) were prepared by diluting with water. The standard solutions for the calibration curve were prepared by diluting the derivatised stock solution (2 mg l -1 ) into six volumetric flasks with n-heptane corresponding to the six calibration points of 40, 70, 100, 130, 160 and 200 mg l -1 of cyclamic acid. Commercial bleach with active chloride was used for the derivatisation procedure.
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10

Antioxidant Assays of Streptokinase

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Lyophilized streptokinase (1,500,000 IU) was obtained from Square Pharmaceuticals Ltd., Dhaka, Bangladesh. Gallic acid, quercetin, and ferric chloride were obtained from Sigma Chemical Co., Saint Louis, MI, USA. Potassium ferricyanide, sodium carbonate, and ascorbic acid were purchased from Merck KGaA, Frankfurt, Darmstadt, Germany, and SD Fine Chem. Ltd., Kolkata, West Bengal, India. ascorbic acid was purchased from SD Fine Chem. Ltd., Kolkata, West Bengal, India, and Potassium ferricyanide from May and Backer, Dagenham, UK. Methanol, Folin-Ciocalteu reagent, sodium carbonate, and Potassium ferricyanide were purchased from Merck KGaA, Frankfurt, Darmstadt, Germany. All chemicals were of analytical reagent grade.
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