Thiostrepton

Pharmacological inhibitor, thiostrepton was purchased from Santa Cruz Biotechnology, Inc (Dallas, TX), volasertib (BI 6727) from Selleckchem (Houston, TX) and AS1842856 from Cayman Chemical (Ann Arbor, Michigan). Silencer Select siRNAs (targeting FOXM1, PLK1, FOXO1 and negative control), Lipofectamine RNAiMAX and Opti MEM were purchased from Thermo Fisher Scientific (Waltham, MA). MTT assay kits were purchased from ATCC (Manassas, VA) and BrdU assay kits were purchased from BioVision, Inc. (Milpitas, CA).

Nine drugs were purchased from Santa Cruz Biotechnology, Inc.: Meticrane, tolazamide, β-sitosterol, memantine hydrochloride (herein referred to as memantine), valproic acid, letrozole, todralazine, thiostrepton, and levocabastine. Niridazole was not available. For in vitro screening, all compounds were dissolved in 100% dimethyl sulfoxide (DMSO) to a stock concentration of 250 mM. All stock solutions were subsequently diluted in complete DMEM to nine working solutions ranging from 0.2 to 1312.3 μM. This dose spectrum covered well below and above the reported dose levels for all drugs described in cMap. PLX4720 (ChemieTek) was solubilized and diluted in a similar manner to nine working solutions ranging from 0.01 to 1562.5 μM. Prior to in vitro testing, we pre-warmed (37 °C) and sonicated all working solutions.

The pharmacological inhibitor thiostrepton was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Pharmacological inhibitors adavosertib, RO-3306, and volasertib (BI 6727) were purchased from Selleckchem (Houston, TX, USA). Pharmacological inhibitors aphidicolin and nocodazole were purchased from Cayman Chemical (Ann Arbor, MI, USA). Silencer Select siRNAs targeting FOXM1 (s5250), PLK1, Myt1 (s224087), Wee1 (s21), CDC25A (s2750), CDC25B (s2754), and CDC25C (s2758) and negative control, Lipofectamine RNAiMAX and Opti MEM were purchased from Thermo Fisher Scientific (Waltham, MA, USA). CDC2 siRNA (Cat # 3500S) was purchased from Cell Signaling Technologies (Danvers, MA, USA).