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43 protocols using ph meter

1

Physiochemical Analysis of Stored Yogurt

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Physiochemical parameters of yogurt samples during the cold storage were determined using standardized instrumental methods [25 ]. The pH was determined with a Metrohm pH meter (Metrohm AG, Herisau, Switzerland) at room temperature. Total solids, total fat, and titratable acidity of the samples at different storage times were determined as described [26 ]. Syneresis of the yogurt samples at different storage times was determined as described [27 ]. Briefly, yogurt samples (30 g) were centrifuged at 1677× g for 10 min, and then the supernatant was poured off, weighed, and recorded as a percentage syneresis.
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2

Quantification of Food Preservatives by HPLC

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Individual stock standard solutions of sodium benzoate and potassium sorbate were prepared in deionized water (1000 mg/kg) and natamycin stock standard was in methanol (1000 mg/kg). The mixed standard solutions were prepared by diluting the stock solutions appropriately with mobile phase to give a concentration between 5 mg/kg and 40 mg/kg.
The pH of samples was measured by 827 model of Metrohm pH meter according to Iranian national standard (ISIRI) [17 ]. A mass of 10 g sample was weighted in a beaker and diluted with 35 mL solution of acetic acid 1% and methanol (35 : 65 v/v) and then shaken for 15–30 min on stirrer. Then, all content of beaker was transferred to the 50 mL volumetric flask and diluted with extraction solution. Finally, the prepared solution was filtered with 0.45 μm PVDF syringe filter and was injected to HPLC.
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3

Characterization of Dust-Emitting Soils

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Soils were sampled from two sites that are undisturbed and associated with dust emission in the field: Ze’elim sandy area (31.16° E/34.53° N) at the western Negev Desert [11 ], and the Yamin plateau (31.04° E/35.08° N) at the northeastern Negev Desert in Israel [12 ]. The soil samples were analyzed for elemental composition by X-ray fluorescence (XRF) using an Axios spectrometer (PANanalytical, Malvern, UK). Mineralogical phase identification was performed by X-ray powder diffraction (XRPD) using an Empyrean Philips 1050/70 diffractometer (PANanalytical, Malvern, UK). Particle size distribution (PSD) was performed by laser diffraction using Analysette 22 MicroTec Plus (Fritsch International, Idar-Oberstein, Germany). XRF, XRPD and PSD analyses were performed at the Ben-Gurion University of the Negev in Beer-Sheva. pH was measured using a Metrohm pH meter (Metrohm, Herisau, Switzerland). Water content in soils was measured gravimetrically. Total organic content (TOC) was determined by titration of the dissolved organics with ammonium iron sulfate using an 848 Titrino plus (Metrohm, Herisau, Switzerland) at the Geological Survey of Israel.
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4

pH Monitoring of Immersed Samples

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On Days 0, 7, 14, 21, and 28, a Metrohm™ pH meter was used to measure the pH of the AS containing the immersed samples. Prior to use, the pH meter was calibrated for pH 4 and 7, according to the standard NIST pH buffering solution.
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5

Thermal Stability and Physicochemical Properties of Emulsion Gels

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The thermal stability (water and fat binding properties) of the emulsion gels was measured in triplicate (Herrero et al., 2014) . Samples were stuffed into tubes, which were hermetically sealed and heated in a water bath for 30 min at 70 ºC. They were then opened and left to stand upside down (for 50 min) to release the separated fat and water onto a plate. Matrix stability, as total fluid release (TFR), was expressed as % of initial sample weight.
The pH was determined using an 827 Metrohm pH-meter (MetrohmAG, Switzerland) on homogenates of emulsion gels in distilled water in a ratio of 1:10 w/v. For each sample, two homogenates were prepared on which three measurements were performed.
Surface colour determination (CIE-LAB tristimulus values, lightness, L*; redness, a* and yellowness, b*) was evaluated (Konica Minolta CM-3500d colorimeter. Konica Minolta, Madrid, Spain) on cross-sections of the samples placed in glass Petri dishes (9 mm diameter). Nine determinations were carried out for each type of emulsion gel.
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6

Measuring Sample pH Using Buffers

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The pH of the samples was measured using a pH meter (Metrohm, Switzerland). The amount of 10 g of the homogenized samples was poured into the beaker and measured by a pH meter which was initially calibrated at 20°C by buffers 4 and 9 [18 ].
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7

Physicochemical Characterization of Vinegar

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The pH values were measured using a pH meter (Metrohm, Herisau, Switzerland). Total acids were determined according to the GB/T 12456-2008. The contents of amino nitrogen in vinegar samples were determined by formaldehyde titration (GB/T 5009.235-2016). The reducing sugar contents in ZAV were calculated by Fehling’s method. The contents of non-volatile organic acids and soluble solids were detected according to the methods of National Standard (GB/T18623-2011; GB/T18187-2000).
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8

Potentiometric Titration of Carboxymethyl Cellulose

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The DS value of each sample was measured by potentiometric titration using pH meter (Metrohm, Switzerland). Dry CMC (0.20 g) was dissolved in 20.00 ml of hydrochloric acid standard solution (0.1000 mol/l). A standard solution of sodium hydroxide was used for titration. The following equation was used to calculate the total DS of the sample (Sun and Wang 2006 (link)): TotalDS=V2-V1×C×molarweightofchitosanresidue1000W, where C is the concentration of sodium hydroxide standard solution (mol/l), V1 the sodium hydroxide volume for titrating excessive hydrochloric acid (ml), and V2 is the volume corresponding to titration terminal of COOH and W is the weight of the sample (g).
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9

pH-Dependent NMR Sample Preparation

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The pH meter readings were recorded using a Metrohm pH meter, equipped with a Metrohm 6.0234.110 combined glass electrode. A four-point calibration using pH 2.00, pH 4.00, pH 7.00 and pH 11.00 standard buffer solutions were performed directly before pH adjustments. The pH measurements were performed in 25 mL vessels with proper stirring before transferring 600 μL sample solutions into the NMR tubes. Solutions were prepared in the pH range of 1.50–8.70 with 0.30 increments using 0.9 M, 0.5 M and 0.1 M sodium hydroxide and hydrochloric acid solutions in H2O:D2O = 9:1.
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10

Comprehensive Electrochemical Measurements

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All electrochemical measurements were carried out in a 50 mL glass cell fixed in mounted head with three-electrode system. It consisted of the working electrode (RGO/GCPE if not stated otherwise), Ag/AgCl/3 M KCl reference and Pt-sheet auxiliary electrodes; all being in connection with an electrochemical analyser (model PGSTAT101, Autolab-Metrohm; Prague, Czech Rep.) that had been controlled by the software ("Nova 1.11" version, Autolab). Whenever needed, the pH values were measured with a pH meter, the glass electrode, and a series of the standards for calibration in an interval of pH 1–11 (all from Metrohm; Prague, Czech Republic).
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