Interleukin 6 (il 6)

Manufactured by Mesoscale

Sourced in United States

IL-6 is a recombinant protein that functions as a cytokine involved in the immune response and inflammation processes. It is commonly used in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Il 1β, by Mesoscale (4 mentions) Ifn γ, by Mesoscale (4 mentions) Tnf α, by Mesoscale (3 mentions) Interleukin 2 (il 2), by Mesoscale (2 mentions) D dimer, by Diagnostica Stago (2 mentions) Il 10, by Mesoscale (2 mentions) Abx pentra 400 autoanalyzer, by Horiba (1 mentions) Human insulin specific ria, by Merck Group (1 mentions) Apelin 12, by Phoenix Pharmaceuticals (1 mentions) Vaspin, by Adipogen (1 mentions) Nesfatin 1, by Phoenix Pharmaceuticals (1 mentions) Mcp 1, by Mesoscale (1 mentions) Il 1α, by Mesoscale (1 mentions) Soluble cd14 scd14, by R&D Systems (1 mentions) Qdr 4500a, by Hologic (1 mentions) Elecsys 2010, by Roche (1 mentions) Hs crp, by Siemens (1 mentions) Tnf α, by Merck Group (1 mentions) Tumor necrosis factor (tnf), by Mesoscale (1 mentions) Kc gro, by Mesoscale (1 mentions)

12 protocols using interleukin 6 (il 6)

Multiplex Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

We performed multiplex assays to measure IL-1β, IL-2, IL-6, TNFα, and IFN-γ purchased from Meso Scale Discovery. All experimental operations were in accordance with standard protocols. R2s of the standard curves for each plate were greater than 0.999.

Bremner J.D., Gurel N.Z., Jiao Y., Wittbrodt M.T., Levantsevych O.M., Huang M., Jung H., Shandhi M.H., Beckwith J., Herring I., Rapaport M.H., Murrah N., Driggers E., Ko Y.A., Alkhalaf M.L., Soudan M., Song J., Ku B.S., Shallenberger L., Hankus A.N., Nye J.A., Park J., Vaccarino V., Shah A.J., Inan O.T, & Pearce B.D. (2020). Transcutaneous vagal nerve stimulation blocks stress-induced activation of Interleukin-6 and interferon-γ in posttraumatic stress disorder: A double-blind, randomized, sham-controlled trial. Brain, Behavior, & Immunity - Health, 9, 100138.

+ Open protocol

+ Expand

Biomarker Analysis in Blood Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected into EDTA and serum tubes and were centrifuged for 15 min at 1000g at 4°C and 21°C, respectively. Aliquots were immediately frozen in liquid nitrogen and subsequently stored at −80°C until analysis. Plasma glucose, FFA, TAG and total cholesterol were analyzed with standard enzymatic methods (ABX Pentra 400 autoanalyzer, Horiba ABX, Montpellier, France). Plasma glycerol was analyzed with an enzymatic assay (Enzytec Glycerol, Roche Biopharm, Basel, Switzerland) automated on a Cobas Fara spectrophotometric autoanalyzer. Plasma insulin, leptin, and adiponectin concentrations were analyzed with commercially available radioimmunoassay (RIA) kits (Human insulin specific RIA, human leptin RIA, human adiponectin RIA, Millipore Corporation, Billerica, MA). Plasma concentrations of IL‐6 (Meso Scale Discovery, Gaithersburg, MD), apelin‐12 (Phoenix pharmaceuticals Inc., Burlingame, CA), RBP4 (R&D systems, Minneapolis, MN) and Vaspin (Adipogen, San Diego, IL) were determined by ELISA. The apelin‐12 ELISA has 100% cross‐reactivity with human apelin‐12, apelin‐13, and apelin‐36. Serum ACE activity was measured with a standard enzymatic assay (Bühlmann, Basel, Switzerland) while serum nesfatin‐1 (Phoenix pharmaceuticals Inc., Burlingame, CA) concentrations were measured by ELISA.

Vink R.G., Roumans N.J., Mariman E.C, & van Baak M.A. (2017). Dietary weight loss‐induced changes in RBP4, FFA, and ACE predict weight regain in people with overweight and obesity. Physiological Reports, 5(21), e13450.

+ Open protocol

+ Expand

Measuring Neuroinflammatory Markers in ART-Naïve Individuals

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

We selected monocyte and macrophage activation markers, as well as specific inflammatory markers that have previously been associated with NCI in ART-naïve individuals35 (link)36 (link)37 (link). Levels of selected monocyte activation and inflammatory markers were measured in CSF and blood plasma by enzyme-linked immunosorbent assay (ELISA): sCD163 (Trillium Diagnostics, Brewer, ME, USA); or by electrochemiluminescence multiplex assay: MCP-1, IL-8, IL-6 and TNF-α, (Meso Scale Diagnostics, Rockville, MD, USA), according to the manufacturer’s procedures.

Oliveira M.F., Murrel B., Pérez-Santiago J., Vargas M., Ellis R.J., Letendre S., Grant I., Smith D.M., Woods S.P, & Gianella S. (2015). Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART. Scientific Reports, 5, 17094.

+ Open protocol

+ Expand

Profiling Immune Cytokine Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

1.5 × 10⁶ Jurkat, THP1 (ATCC), THP1-Dual or THP1-Dual STING KO cells were treated with 3x GI₅₀ V158411 or 50 μg/mL cGAMP for 24 h. Cells were harvested by centrifugation and lysed in 100 μL ice cold lysis buffer on ice for 30 min. Cell debris was pelleted and 25 μL of the supernatant added to a 10 spot U-PLEX biomarker plate pre-conjugated with anti-TNF-α, MCP-3, IL-6, IL-22, IL-18, IL-1β, IL-1α, IFN-γ, IFN-β and IFN2a capture antibodies (MesoScale Discovery, Rockville, MD). The plate was developed according to the manufacturer’s instructions and read using a SECTOR Imager 2400.

Brooks T., Wayne J, & Massey A.J. (2021). Checkpoint Kinase 1 (Chk1) inhibition fails to activate the Stimulator of Interferon Genes (STING) innate immune signalling in a human coculture cancer system. Molecular Biomedicine, 2, 19.

+ Open protocol

+ Expand

Biomarker Evaluation in HIV Cohort

Check if the same lab product or an alternative is used in the 5 most similar protocols

At each study visit, blood was collected into EDTA tubes and tested for CD4 count (PIMA, Alere) and HIV-1 RNA viral load (Cobas Taqman platform in Uganda and the Roche CAP/CTM HIV-1 v2 assay in South Africa). Additional blood was centrifuged for plasma separation, and frozen at −80^oC. Due to resource constraints that prevented us from testing the entire cohort, only non-pregnant individuals underwent additional testing of cryopreserved plasma for: 1) IL-6 (MesoScale Discovery, Rockville, MD); 2) soluble CD14 (sCD14, R&D Systems, Minneapolis, MN); and 3) D-dimer (Diagnostica Stago, Parsippany, NY. Biomarker assays were performed at the Laboratory for Clinical Biochemistry Research at the University of Vermont.

Siedner M.J., Bwana M.B., Asiimwe S., Musinguzi N., Castillo-Mancilla J., Amanyire G., Tracy R.P., Bangsberg D.R., Orrell C, & Haberer J.E. (2019). Inflammatory biomarkers prior to antiretroviral therapy as prognostic markers of 12-month mortality in South Africa and Uganda. AIDS (London, England), 33(13), 2043-2048.

+ Open protocol

+ Expand

Biomarker Testing in HIV Cohort

Check if the same lab product or an alternative is used in the 5 most similar protocols

At each study visit, blood was collected into EDTA tubes and tested for CD4⁺ cell count (Pima, Abbott Laboratories, Lake Bluff, Illinois, USA) and HIV-1 RNA viral load [Cobas Taqman platform in Uganda (Applied Biosciences, Beverley Hills, California, USA) and the CAP/CTM HIV-1 v2 assay (Roche Laboratories, Basel, Switzerland) in South Africa]. Additional blood was centrifuged for plasma separation, and frozen at -80^oC. Due to resource constraints that prevented us from testing the entire cohort, only nonpregnant individuals underwent additional testing of cryopreserved plasma for interleukin (IL-6; MesoScale Discovery, Rockville, Maryland, USA); soluble CD14 (sCD14; R&D Systems, Minneapolis, Minnesota, USA); and D-dimer (Diagnostica Stago, Parsippany, New York, USA). Biomarker assays were performed at the Laboratory for Clinical Biochemistry Research at the University of Vermont.

+ Open protocol

+ Expand

DXA Scanning for Body Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols

All patients underwent whole-body DXA scanning (Hologic, QDR 4500A) at baseline (week 0) and at week 48 to assess total body and subtotal (total minus head) BMD, as well as fat and lean body mass. Fasting plasma samples were collected and frozen at −70°C. Markers of inflammation and bone turnover were measured at weeks 0, 16, and 48. All assays were performed blinded to treatment group and BMD measurements. Inflammatory markers measured were: soluble tumor necrosis factor (TNF)-α, interleukin 1b (IL1-B), and IL-6 (Meso Scale Discovery, Rockville, MD). BTMs measured included C-terminal telopeptide of type 1 collagen (CTX, marker of bone resorption), and N-terminal type 1 procollagen (P1NP, marker of bone formation). CTX was measured using a luminometric assay on Elecsys 2010 (Hoffmann-La Roche); reference range: men 0.158–0.584 ng/mL, pre-menopausal women 0.162–0.573 ng/ml, post-menopausal women 0.330–1.008 ng/ml; Inter-assay coefficient of variance <8.9%. P1NP was measured with radioimmunoassay (UniQ P1NP RIA kit; Orion Diagnostica) reference range 25.91–132.5 ng/mL; Inter-assay coefficient of variance <12.4%.

Bedimo R.J., Drechsler H., Jain M., Cutrell J., Zhang S., Li X., Farukhi I., Castanon R., Tebas P, & Maalouf N.M. (2014). The RADAR Study: Week 48 Safety and Efficacy of RAltegravir Combined with Boosted DARunavir Compared to Tenofovir/Emtricitabine Combined with Boosted Darunavir in Antiretroviral-Naive Patients. Impact on Bone Health. PLoS ONE, 9(8), e106221.

+ Open protocol

+ Expand

Biomarker Evaluation in HFpEF Subtypes

Check if the same lab product or an alternative is used in the 5 most similar protocols

hs‐CRP, IL‐6,TNF‐α and PTX3 were measured at baseline exams by the NHLBI Heart Failure Research Network Biomarker Core Laboratory at the University of Vermont using commercially available kits (hs‐CRP, Siemens, Indianapolis; IL‐6, Meso Scale Discovery, Gaithersbury, MD; TNF‐α, EMD Millipore, Billerica, MA; PTX3, R&D systems, Minneapolis, MN). Samples were shipped on dry ice and stored at −80°C until analysis at the Biomarker Core Laboratory.
In addition to determining whether there are differences in pro‐inflammatory biomarkers in AD‐HFpEF versus S‐HFpEF, we also tested whether biomarker levels are associated with echocardiographic‐Doppler abnormalities of left ventricular diastolic function in all comers and are predictive of clinical outcomes in AD‐HFpEF. In the DOSE and ROSE trials short‐term clinical outcomes included urine volume, change in cystatin‐C, change in creatinine, change in NT‐proBNP, and change in weight over the 72 hours after randomization. In addition, post‐discharge clinical status with respect to survival and re‐hospitalizations was determined by telephone call 60 days after randomization for each trial.

Abernethy A., Raza S., Sun J., Anstrom K.J., Tracy R., Steiner J., VanBuren P, & LeWinter M.M. (2018). Pro‐Inflammatory Biomarkers in Stable Versus Acutely Decompensated Heart Failure With Preserved Ejection Fraction. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(8), e007385.

+ Open protocol

+ Expand

Intradermal LPS Administration Safety and Cytokine Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

Safety and tolerability were monitored by tracking adverse events, measuring vital signs, and standard laboratory tests (i.e. haematology) at 3, 6, 10, 24 and 48 hours after LPS administration. Circulating cytokines (IL‐1β, IL‐6, IL‐8, IL‐10, IFN‐γ and TNF; Meso Scale Discovery, Rockville, Maryland, USA) were measured in blood samples to detect a possible systemic effect of intradermal LPS administration. The blood samples for cytokine analysis were analysed in 2 batches with each having a different dilution, therefore the lower limit of quantitation (LLOQ) of the samples was the following: IL‐1β 0.298 or 0.745 pg/mL; IL‐6 1.52 or 3.81 pg/mL; IL‐8 1.25 or 3.12 pg/mL; IL‐10 0.702 or 1.76 pg/mL; IFN‐γ 10 or 25 pg/mL; and TNF 0.760 or 1.90 pg/mL.

Buters T.P., Hameeteman P.W., Jansen I.M., van Hindevoort F.C., ten Voorde W., Florencia E., Osse M., de Kam M.L., Grievink H.W., Schoonakker M., Patel A.A., Yona S., Gilroy D.W., Lubberts E., Damman J., Feiss G., Rissmann R., Jansen M.A., Burggraaf J, & Moerland M. (2021). Intradermal lipopolysaccharide challenge as an acute in vivo inflammatory model in healthy volunteers. British Journal of Clinical Pharmacology, 88(2), 680-690.

+ Open protocol

+ Expand

BALF Cytokine Profiling via MSD

Check if the same lab product or an alternative is used in the 5 most similar protocols

The BALF supernatant samples were analysed using MSD pro-inflammatory panel V-Plex including the following analytes: IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO and TNF-α (Meso Scale Diagnostics, Rockville, MD). The analysis was performed according to the manufacturer’s instruction.

Wronski S., Dannenmaier J., Schild S., Macke O., Müller L., Burmeister Y., Seilheimer B, & Müller M. (2018). Engystol reduces onset of experimental respiratory syncytial virus-induced respiratory inflammation in mice by modulating macrophage phagocytic capacity. PLoS ONE, 13(4), e0195822.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!