Masson s trichrome

Manufactured by Bio-Optica

Sourced in Italy, United States

Masson's trichrome is a histological staining technique used to differentiate collagen fibers, muscle, and nuclei in tissue samples. It provides a clear visualization of the structural components within the sample.

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Lab products found in correlation

F4 80 bm 8, by Santa Cruz Biotechnology (3 mentions) Myeloperoxidase mpo, by Abcam (3 mentions) Evos xl core cell imaging system, by Thermo Fisher Scientific (3 mentions) Dm6 microscope, by Leica (2 mentions) Las x navigator software, by Leica (2 mentions) Nf κb p65, by Bio-Rad (1 mentions) Dm750 clinical microscope, by Leica (1 mentions) Roti histofix, by Carl Roth (1 mentions) Cp64 0ce, by Sartorius (1 mentions) Aniline blue, by Bio-Optica (1 mentions) Hematoxylin eosin, by Merck Group (1 mentions) Masson s trichrome, by Santa Cruz Biotechnology (1 mentions) Dfc495, by Leica (1 mentions) Image pro plus program, by Media Cybernetics (1 mentions) Masson s trichrome, by Media Cybernetics (1 mentions) Sirius red, by Bio-Optica (1 mentions) Paraformaldehyde pfa, by Merck Group (1 mentions) Microtome, by Leica (1 mentions) Confocal microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

Spelling variants of this product

Masson trichrome (19 citations) Masson’s trichrome stain (6 citations) Masson’s trichrome staining (4 citations) Masson's Trichrome kit (4 citations) Masson’s Trichrome staining kit (3 citations) Masson’s trichrome stain kit (2 citations) Masson’s trichrome method (2 citations)

25 protocols using masson s trichrome

Quantitative Renal Histopathology Analysis

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Kidney segments were fixed in 4% formalin for 48 h, then embedded in paraffin and cut. Kidney sections were deparaffinized, rehydrated and stained with Masson’s trichrome (Bio-Optica, Milano, Italy). Histological analyses were done by two Pathologists (AFT and DB) blinded to study groups. Damaged area quantification of Masson’s trichrome staining was performed as described by Chen et al. using the ImageJ software^{34 (link)}.
Immunohistochemistry staining was performed as previously described^{5 (link)}. Primary antibodies against F4/80 (BM-8) (diluted 1:30; Santa Cruz biotechnology Inc., Dallas Texas, USA) and Myeloperoxidase (MPO) (diluted 1:50; Abcam, Cambridge, UK), NF-κB p65 (Bio-Rad, CA, USA), IL-1 and IL-6 (GeneTex, CA, USA) were used for the immunohistochemistry staining. For image processing, Topika Analysis software (Topika, Tel Aviv, Israel) was used. Area quantification of immunohistochemical staining was performed using an ImageJ software as described.

Landau D., Khalilia J., Arazi E., Tobar A.F., Benharroch D., Golan-Goldhirsh A., Gopas J, & Segev Y. (2024). A Nuphar lutea plant active ingredient, 6,6′-dihydroxythiobinupharidine, ameliorates kidney damage and inflammation in a mouse model of chronic kidney disease. Scientific Reports, 14, 7577.

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Histological Analysis of Decellularized Heart Scaffolds

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When the software application considered the decellularization complete, the hearts were fixed in 4% buffered formaldehyde solution (Roti-Histofix, Carl Roth GmbH, Karlsruhe, Germany), paraffin-embedded, and sectioned. Three standardized regions were analyzed: apical, mid-ventricular, and basis-near region. After rehydration, sections were first stained with hematoxylin and eosin (H&E, Sigma-Aldrich, St. Louis, MO, USA; Merck & Co., Kenilworth, NJ, USA). This principle tissue stain colors basophilic structures in blue (cell nuclei) and eosinophilic in pink (extracellular matrix and cytoplasm). In order to validate the protocols’ decellularization efficacy and the integrity of the remaining scaffold, Masson’s trichrome (Bio-Optica, Milano, Italy) staining techniques were also used for visualization of collagenous connective tissue fibers. The results were visualized using a light microscope (Leica DM750 Clinical Microscope, Leica Microsystems, Wetzlar, Germany) and a Panthera L Microscope (Motic, Richmond, CA, USA).

Barbulescu G.I., Buica T.P., Goje I.D., Bojin F.M., Ordodi V.L., Olteanu G.E., Heredea R.E, & Paunescu V. (2022). Optimization of Complete Rat Heart Decellularization Using Artificial Neural Networks. Micromachines, 13(1), 79.

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Histopathological Evaluation of Cardiac Tissue

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For histopathological evaluation of cardiac tissue, mouse hearts were rinsed with PBS and fixed in PFA 4% (CP10; Roth) for at least 24 h. For analysis of the heart/body weight ratio, the pericardium, connective tissue, and vascular remains were excised carefully before hearts were weighed using a microbalance (CP64-0CE; Sartorius). Subsequently, hearts were dehydrated in a graded series of ethanol concentrations. Thereafter, tissues were embedded in paraffin (1.07158; Sigma-Aldrich). Murine sections were stained with hematoxylin (T865.1; Roth) and eosin (X883.1, day 21; Roth) to evaluate infiltration of hematopoietic cells or with Masson’s trichrome (010802, day 63; Bio-Optica) to assess cardiac fibrosis. Acute infiltration was evaluated semiquantitatively using an EAM score (0, no inflammatory infiltrates; 1, small foci of <100 inflammatory cells between myocytes; 2, larger foci of >100 inflammatory cells; 3, >10% of a cross section shows infiltration of inflammatory cells; 4, >30% of a cross section shows infiltration of inflammatory cells) as previously described (Valaperti et al., 2008 (link)). To assess fibrosis by morphometric evaluation, ∼30 fields per section were chosen randomly. Fibrosis was evaluated using a semiquantitative score (0, 0–1%; 1, 1–2%; 2, 2–3%; 3, 3–4%; 4, 4–5%; 5, >5% fibrosis). All analyses were performed in a blinded manner.

Weckbach L.T., Grabmaier U., Uhl A., Gess S., Boehm F., Zehrer A., Pick R., Salvermoser M., Czermak T., Pircher J., Sorrelle N., Migliorini M., Strickland D.K., Klingel K., Brinkmann V., Abu Abed U., Eriksson U., Massberg S., Brunner S, & Walzog B. (2019). Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis. The Journal of Experimental Medicine, 216(2), 350-368.

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Histological Analysis of Tissue Samples

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Fixed native and decellularized tissue samples were frozen in liquid nitrogen with optimum cutting temperature (OCT) compound. Cryosectioned tissue slices (6 μm thick) were used for the histological analyses using a Hematoxylin and Eosin kit (04-061010, BioOptica, Milan, Italy) and Masson’s Trichrome (04-010802, BioOptica, Milan, Italy) to assess the presence of nuclei (black), collagen (blue), cytoplasm and muscle fibres (red); Weigert–van Gieson (long method, 04-051802, BioOptica, Milan, Italy) to display elastic fibres, connective tissue, collagen, and nuclei (black); an Alcian Blue Stain Kit (pH 2.5, Mucin Stain, ab150662, Abcam, Cambridge, UK) for the visualization of sulphated and carboxylated acid mucopolysaccharides and sulphated and carboxylated sialomucins (glycoproteins). Each procedure was performed according to the supplier’s instructions.
Images were acquired with an EVOS XL Core Cell Imaging System (Thermo Fisher Scientific, Waltham, MA, USA).

Casarin M., Fortunato T.M., Imran S., Todesco M., Sandrin D., Borile G., Toniolo I., Marchesan M., Gerosa G., Bagno A., Romanato F., Carniel E.L., Morlacco A, & Dal Moro F. (2022). Porcine Small Intestinal Submucosa (SIS) as a Suitable Scaffold for the Creation of a Tissue-Engineered Urinary Conduit: Decellularization, Biomechanical and Biocompatibility Characterization Using New Approaches. International Journal of Molecular Sciences, 23(5), 2826.

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Histological Analysis of Human Tongue Tissue

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Tissue samples of 5 fresh specimens (3 male and 2 female) were fixed in Bouin fixative for 24 hr, dehydrated and routinely embedded in paraffin. As in previous studies on tongue (Abbate et al., 2006 (link), 2017 (link), 2020b (link), 2020a (link), 2012b (link), 2012a (link)), about 10 µm thick transversal, horizontal and sagittal serial sections were obtained using Leica RM 2135 microtome (Leica microsistems Nussloch GmbH). Sections were mounted on microscope slides and stained with Masson's trichrome and with aniline blue 04‐010802 (Bio‐Optica). Weigert's iron haematoxylin for nuclei staining and aniline blue for connective tissue stain were used. After rinsing in distilled water, the sections were submitted to the reagents, as mentioned in the product datasheet, washed in distilled water and rapidly dehydrated through ascending alcohols, clarified in xylene and mounted using Eukitt mounting medium # ref. 09‐00250 (Bio‐Optica). Slides were observed under a Leica DMRB light microscope (Leica microsistems GmbH), and sections were captured with Leica camera MC 120 HD (Leica microsistems GmbH) (Garcia‐Suarez et al., 2018 (link)).

Abbate F., Guerrera M.C., Levanti M., Laurà R., Aragona M., Mhalhel K., Montalbano G, & Germanà A. (2021). Morphological characteristics of the blackspot seabream (Pagellus bogaraveo) tongue: A structural and immunohistochemical study. Anatomia, Histologia, Embryologia, 51(1), 103-111.

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Histological Analysis of Tissue Samples

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For histological analysis, tissues were subjected to hematoxylin and eosin staining and observed by competent pathologists using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy), associated with Leica LAS X Navigator software (Leica Microsystems SpA, Milan, Italy). Histological injuries were scored as previously reported [12 (link),32 (link),33 (link)]. Paraffin-embedded skin tissues with a thickness of 5 μm were stained with Masson’s trichrome, according to the manufacturer’s protocol (Bio-Optica, Milan, Italy) [34 (link),35 (link)].

D’Amico R., Cordaro M., Fusco R., Peritore A.F., Genovese T., Gugliandolo E., Crupi R., Mandalari G., Caccamo D., Cuzzocrea S., Di Paola R., Siracusa R, & Impellizzeri D. (2022). Consumption of Cashew (Anacardium occidentale L.) Nuts Counteracts Oxidative Stress and Tissue Inflammation in Mild Hyperhomocysteinemia in Rats. Nutrients, 14(7), 1474.

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Histological Analysis of Cardiac Fibrosis

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Hearts were fixed in 10% formalin overnight, embedded in paraffin and sectioned at 5 µm thickness. To detect collagen deposition, sections were stained with Masson’s Trichrome (Bio-Optica, Milan, Italy). Photomicrographs of the sections were evaluated for interstitial collagen fractions using computer-assisted image analysis systems (Adobe Photoshop).
Metachromatic staining with toluidine blue (0.1%, pH 2.3, for 3 min) was employed to detect mast cell density and degranulation [31 (link)]. Mast cell density was determined counting the total number of mast cells per field. Mast cell degranulation was expressed as the number of degranulating mast cells normalized to total number of mast cells.

Deshwal S., Forkink M., Hu C.H., Buonincontri G., Antonucci S., Di Sante M., Murphy M.P., Paolocci N., Mochly-Rosen D., Krieg T., Di Lisa F, & Kaludercic N. (2018). Monoamine oxidase-dependent endoplasmic reticulum-mitochondria dysfunction and mast cell degranulation lead to adverse cardiac remodeling in diabetes. Cell Death and Differentiation, 25(9), 1671-1685.

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Kidney Histopathological Analysis

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Kidney segments were fixed in 4% formalin for 48 h, then embedded in paraffin and cut. Kidney sections were deparaffinized, rehydrated and stained with hematoxylin & eosin (Sigma-Aldrich, Saint Louis, MO, USA) or Masson’s trichrome (Bio-Optica, Milano, Italy). Fibrotic area quantification of Masson’s trichrome staining was performed as described by Chen et al. using an ImageJ software⁵².
Immunohistochemistry staining was performed as previously described ^{50 (link)}, primary antibodies against F4/80 (BM-8) (diluted 1:30; Santa Cruz biotechnology Inc., Dallas Texas, USA) and Myeloperoxidase (MPO) (diluted 1:50; Abcam, Cambridge, UK) were used for the immunohistochemistry staining. For image processing, Cellsense Entry software (MATIMOP, Tel Aviv, Israel) was used.

Bandach I., Segev Y, & Landau D. (2021). Experimental modulation of Interleukin 1 shows its key role in chronic kidney disease progression and anemia. Scientific Reports, 11, 6288.

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Histological Analysis of Myocardial Fibrosis and Amyloid

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Myocardial fragments were fixed in 4% buffered formalin, processed by a standard technique, and paraffin-embedded sections (3–4 μm thick) were stained with hematoxylin and eosin, Masson’s trichrome (Bio-Optica), Sirius Red (Bio-Optica) and used for histological examination. Masson’s trichrome-stained sections were used in total for morphometric evaluation of percent fibrosis at × 100 using the Image-Pro Plus program (version 6.0.0.260; Media Cybernetics, USA). Sirius Red-stained sections were used for a semi-quantitative 5-point assessment of the amyloid deposits distribution in the interstitium around blood vessels and cardiomyocytes: 0—no deposits; 1—deposits were around single cardiomyocytes; 2—around small groups of cardiomyocytes; 3—around half of cardiomyocytes and single vessels; 4—around more than 75% of cardiomyocytes and blood vessels. The slides were examined by light microscope Leica DMRB with Leica DFC495 camera and Leica PL FLUOTAR 10 × /0.3 and Leica N PLAN 40 × /0.65 (Leica Microsystems Gmbh, Austria).

Sukhacheva T.V., Nizyaeva N.V., Samsonova M.V., Cherniaev A.L., Burov A.A., Iurova M.V., Shchegolev A.I., Serov R.A, & Sukhikh G.T. (2021). Morpho-functional changes of cardiac telocytes in isolated atrial amyloidosis in patients with atrial fibrillation. Scientific Reports, 11, 3563.

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Histopathological Analysis of Myocarditis

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The excised myocardium was kept in formalin and the sections were then embedded in paraffin [19 (link),20 (link),21 (link),22 (link)]. The heart tissue was sectioned and stained with hematoxylin and eosin. The sections were scored for myocarditis as follows in blinded (score 0–4) [13 (link)]. Moreover, the area of inflammatory cells was evaluated using image J (National Institutes of Health, Bethesda, MD, USA), which was shown as the ratio of area of inflammatory cells to that of total area [23 (link)]. Paraffin-embedded heart tissues were also stained with Masson’s trichrome according to the manufacturer’s protocol (Bio-Optica, Milan, Italy).

D’Amico R., Fusco R., Cordaro M., Interdonato L., Crupi R., Gugliandolo E., Di Paola D., Peritore A.F., Siracusa R., Impellizzeri D., Cuzzocrea S, & Di Paola R. (2022). Modulation of NRF-2 Pathway Contributes to the Therapeutic Effects of Boswellia serrata Gum Resin Extract in a Model of Experimental Autoimmune Myocarditis. Antioxidants, 11(11), 2129.

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