Ha antibody sc7392

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The HA antibody (sc7392) is a primary antibody that recognizes the hemagglutinin (HA) tag. It is commonly used in various research applications to detect and study proteins that have been engineered to contain the HA tag.

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Lab products found in correlation

P53 polyclonal antibody fl393, by Santa Cruz Biotechnology (1 mentions) Ab33906, by Abcam (1 mentions) Antibody against flag m2, by Merck Group (1 mentions) Pcdna3.1 circrna mini vector, by Addgene (1 mentions) Pyaps127, by Cell Signaling Technology (1 mentions) Lats2, by Cell Signaling Technology (1 mentions) Lats1, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) P yap s397, by Cell Signaling Technology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Yap taz, by Cell Signaling Technology (1 mentions) Random hexamer, by Thermo Fisher Scientific (1 mentions) Dynabeads protein g, by Thermo Fisher Scientific (1 mentions) Rnasin plus rnase inhibitor, by Promega (1 mentions) M mlv reverse transcriptase, by Promega (1 mentions)

3 protocols using ha antibody sc7392

Plasmid Constructs for p53 Regulation

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Plasmid of pGL4-p53 was purchased from Promega. The Plasmids of pCDNA3.1-CDR1as was a gift of Dr. Nikolaus Rajewsky. Plasmids of Myc-p53, Myc-MDM2, PCDH-p53 and HA-Ub were a gift of Dr. Zhang Lingqiang. The pCDNA3.1(+) circRNA mini vector was purchased from Addgene (60648). The plasmid of HA-MDM2 was purchased from Sino Biological (HG11206-CY). All expression constructs were verified by DNA Sequencing.
Antibodies against p53 (DO-1) (ab1101), MDM2 (ab3110), or PUMA (ab33906) were purchased from Abcam Inc. The p53 polyclonal antibody (FL393) and the Myc antibody (9E10), and the HA antibody (sc7392) were purchased from Santa Cruz Biotechnology. The antibody against Flag (M2) was purchased from Sigma. The antibody against GAPDH (AC027) and p21 (A11877) were purchased from Abclonal. The antibody against Phospho-Histone H2A.X (Ser139) (2577 s) was purchased from CST.

Lou J., Hao Y., Lin K., Lyu Y., Chen M., Wang H., Zou D., Jiang X., Wang R., Jin D., Lam E.W., Shao S., Liu Q., Yan J., Wang X., Chen P., Zhang B, & Jin B. (2020). Circular RNA CDR1as disrupts the p53/MDM2 complex to inhibit Gliomagenesis. Molecular Cancer, 19, 138.

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Antibody Reagents for HIPPO Pathway

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Antibodies to MARK2 (#9118), phospho-MARK activation loop (#4836) YAP/TAZ (#8418), pYAP S127 (#4911), pYAP S397 (#13619), LATS1 (#9153), LATS2 (#13646), pLATS1/2 Thr1079/1041 (#8654), MOB1 (#3863), phosphor-MOB1 (Thr35, #8699), MST1 (#3682), phosho-MST1/2 (Thr183/180, #3681)) and Myc Tag (#2278) were from Cell Signaling Technology (Danvers, MA, USA). HA antibody (#sc-7392) was from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-USP21, anti-MARK1, anti-actin, HA-, and Myc-conjugated beads were from Sigma-Aldrich (St Louis, MO, USA).

Nguyen H.T., Kugler J.M., Loya A.C, & Cohen S.M. (2017). USP21 regulates Hippo pathway activity by mediating MARK protein turnover. Oncotarget, 8(38), 64095-64105.

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Immunoprecipitation of FIP37-HA for RNA Analysis

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RIP was performed as previously described (Koster and Staiger, 2014 (link)) with minor modifications. Briefly, 5-day-old fip37–4 gFIP37–4HA seedlings were fixed on ice for 30 min in 1% formaldehyde supplemented with RNasin Plus RNase inhibitor (Promega) and phenylmethylsulfonyl fluoride under a vacuum. Fixed tissues were homogenized, and the extract was subjected to immunoprecipitation with HA antibody (sc-7392, Santa Cruz) bound to Dynabeads Protein G (Invitrogen). The input RNA and immunoprecipitated RNA were reverse transcribed with random hexamers (Invitrogen) using M-MLV Reverse Transcriptase (Promega). Relative enrichment of each gene was determined by quantitative real-time PCR and calculated first by normalizing the amount of a target cDNA fragment against that of TUB2 as an internal control, and then by normalizing the value for immunoprecipitated samples against that for the input.

Shen L., Liang Z., Gu X., Chen Y., Teo Z.W., Hou X., Cai W.M., Dedon P.C., Liu L, & Yu H. (2016). N6-Methyladenosine RNA Modification Regulates Shoot Stem Cell Fate in Arabidopsis. Developmental cell, 38(2), 186-200.

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