Tissue specimens were fixed with 4% (v/v) formalin solution. Tissue was embedded in paraffin. Approximately 4 μm−thick sections were subjected to stained with Masson’s Trichrome for collagen and keratin fibers analysis. After tissue staining, slides were examined at 200 X magnification by Nikon DS–Fi3 (Nikon).
Ds fi3
Manufactured by Nikon
Sourced in Japan, United States, United Kingdom, Germany
The Nikon DS-Fi3 is a high-resolution digital camera designed for use in microscopy applications. It features a 5.9-megapixel CMOS sensor and can capture images with a resolution of up to 2880 x 2304 pixels. The camera is compatible with a wide range of Nikon microscopes and supports various image file formats, including JPEG and TIFF.
Automatically generated - may contain errors
Lab products found in correlation
Eclipse ts2, by Nikon (6 mentions)
Nis elements software, by Nikon (6 mentions)
Smz18, by Nikon (6 mentions)
Vs120 s6 w virtual slide scanner, by Olympus (5 mentions)
Eclipse ci l, by Nikon (4 mentions)
Eclipse ti2, by Nikon (3 mentions)
Gbe05 a, by Nikon (3 mentions)
Eclipse ni, by Nikon (3 mentions)
Nis element, by Nikon (3 mentions)
Smz1500, by Nikon (2 mentions)
Nis elements d software, by Nikon (2 mentions)
Eclipse ci microscope, by Nikon (2 mentions)
Neutral buffered paraformaldehyde, by Merck Group (2 mentions)
Smz25, by Nikon (2 mentions)
Af micro nikkor 60 mm f 2.8d, by Nikon (2 mentions)
Genesis mx stm 1 w, by Thorlabs (2 mentions)
C aa achromat aplanat condenser, by Nikon (2 mentions)
Lv150l, by Nikon (2 mentions)
Quantum ventus 532, by Nikon (2 mentions)
Plan fluor 40x, by Nikon (2 mentions)
113 protocols using ds fi3
1
Collagen and Keratin Fiber Analysis
2
Visualizing Tumor Microenvironment with MPIO Labeling
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After in vivo imaging, tumors and lymph nodes were removed and fixed in 4% paraformaldehyde for 24 h followed by cryopreservation through serial submersion in sucrose (10%, 20% and 30%). Sections were then frozen in optimal cutting temperature compound. Tissue was sectioned using a cryostat (10 μm sections). Sections were then stained with PPB for iron and primary F4/80 monoclonal antibody (BM8), (eBioscience™ from Thermo Fisher Scientific, catalog #14-4801-85) with goat anti-rat IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 647 (Thermo Fisher Scientific, catalog #A-21247). The 4T1BGL cells could be visualized as green (GFP) and MPIO particles as blue. A sample of fixed MPIO-labeled cells were visualized under brightfield and fluorescence microscopy to confirm labeling of cells.
Microscopy was performed using a Nikon Eclipse Ci microscope equipped with a Nikon DS-Fi3 high-definition camera (Nikon Instruments Inc. Tokyo, Japan) for fluorescent imaging and CoolSNAP DYNO (Photometrics, AZ, USA) for color and brightfield acquisition and NIS elements BR 5.21.02 software (Nikon). Microscopy images were prepared using Fiji (ImageJ version 2.0.0-rc-69/1.52i).
Microscopy was performed using a Nikon Eclipse Ci microscope equipped with a Nikon DS-Fi3 high-definition camera (Nikon Instruments Inc. Tokyo, Japan) for fluorescent imaging and CoolSNAP DYNO (Photometrics, AZ, USA) for color and brightfield acquisition and NIS elements BR 5.21.02 software (Nikon). Microscopy images were prepared using Fiji (ImageJ version 2.0.0-rc-69/1.52i).
3
Insect Fixation and Cytological Analysis
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In most cases whole individuals were fixed immediately after collecting in 3:1 (ethanol: acetic acid) fixative. A part of the insects was dissected, and the abdomen was immersed in fixative for cytological analysis while the head and thorax were stored in ethanol for molecular analysis of the same individual. Cytology was performed as described by Nokkala et al. (2008) (link) with 30 min Schiff staining and 40 min Giemsa staining.
Chromosome preparations were photographed with Nikon DS-Fi3 camera mounted on Nikon Ci-L microscope using NIS Elements software. Final processing of photomicrographs was made with Corel-PhotoPaint 2018 software.
Chromosome preparations were photographed with Nikon DS-Fi3 camera mounted on Nikon Ci-L microscope using NIS Elements software. Final processing of photomicrographs was made with Corel-PhotoPaint 2018 software.
4
Alizarin Red S Staining for Mineral Detection
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Mineral deposition was examined using an ARS staining assay according to the previously described [74 (link),75 (link)]. Cells were fixed with 4% paraformaldehyde and rinsed with deionised water. The ARS solution at 1% w/v concentration was used to stain the cells for 3 min. The excessive colour from ARS staining was gently washed with deionised water. The culture plates were then flipped and dried overnight. Microscopic images of the mineralisation area were taken with a Microscope ECLIPSE Ts2, Nikon-DS-Fi3, Minato-ku, Tokyo, Japan). Consequently, the staining was eluted using 10% cetylpyridium chloride monohydrate in 10 mM sodium phosphate (Sigma Aldrich, St Louis, MO, USA). The absorbance at 570 nm was examined using a microplate reader (Biotek ELX800, Santa Clara, CA, USA).
5
Whole Embryo Imaging with Microscopy
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Whole embryo images were acquired using a Nikon SMZ1500 stereomicroscope. Images of peripheral blood and tissue sections were acquired using a Nikon Eclipse E600 microscope equipped with a Nikon DS-Fi3 microscope camera and NIS-Elements Documentation software (Nikon Instruments). Embryos were examined with a 0.5X objective; smears with a 100X/1.4 oil-immersion objective; and tissue sections with a 20X/0.60 or 40X/0.75 objectives. Agarose gel thermal images and western blot films were scanned using a HP Scanjet G4010 scanner and HP Easy Scan software v.1.8 (Hewlett Packard). Final images were assembled using Microsoft PowerPoint for Mac v.16 (Microsoft).
6
Microscopic Imaging Acquisition and Analysis
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All the microscopic images were acquired using Nikon Eclipse Ti2 microscope equipped with color camera Nikon DS-Fi3. The objectives used were 10× achromatic objective, numerical aperture 0.25; 20× Plan Fluor dichroic N2, numerical aperture 0.5. Analyses were performed using the software NIS-Elements (version 5.11.01).
7
Quantifying Egg Measurements for Research
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All the eggs were measured when fewer than 20 eggs were collected in the oviposition substrate. Otherwise, a 10% subsample of eggs was visually selected for each oviposition substrate (see Additional File 1, Table 1 for sampling size ).
Each egg was prepared on a microscopic slide using a piece of double-sided adhesive tape. The eggs were observed under a Zeiss Standard 25 microscope equipped with a Nikon DS-Fi3 digital camera (Nikon Corporation) and an additional LED light source. The length and width of each egg were measured in μm with NIS elements software version 4.6 from Nikon Corporation. Each measure was taken three times to avoid observation bias [25 (link)]. Width was measured approximately at one third of the anterior part of the egg, which is both the widest part of the egg and the part where the egg shell breaks when the larvae hatch. Egg volume in ×10−3 mm3 was calculated using the following formula [2 (link), 53 ]:
Each egg was prepared on a microscopic slide using a piece of double-sided adhesive tape. The eggs were observed under a Zeiss Standard 25 microscope equipped with a Nikon DS-Fi3 digital camera (Nikon Corporation) and an additional LED light source. The length and width of each egg were measured in μm with NIS elements software version 4.6 from Nikon Corporation. Each measure was taken three times to avoid observation bias [25 (link)]. Width was measured approximately at one third of the anterior part of the egg, which is both the widest part of the egg and the part where the egg shell breaks when the larvae hatch. Egg volume in ×10−3 mm3 was calculated using the following formula [2 (link), 53 ]:
8
Quantitative analysis of PPARγ immunoreactivity
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The levels of immunoreactivity for PPARγ were in the CA3 Py, CA1 Py, Sub, La, and in the PRh. The staining was performed as previously detailed [31 (link)]. Immunostained sections from −8.04 mm to −5.04 mm Bregma level were evaluated with a Nikon Eclipse CiL (Nikon Instruments) at 20X, and images were digitally captured by a Nikon DS-Fi3 digital camera. PPARγ immunoreactivity was measured as a binary area fraction after discrimination from background staining. Particularly, the binary area fraction (binary area/measured area) was analyzed using NIS-Elements software. Particularly, the binary area represented the sum of areas of all binary objects, whereas the measured area was the region of interest (ROI) within the measurement frame. In all sections, the ROI was manually traced.
9
Microscopic Imaging of Blood Smears
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Images of peripheral blood were acquired using a Nikon Eclipse E600 microscope equipped with a Nikon DS-Fi3 color camera and NIS-Elements Documentation imaging software (Nikon). Smears were examined with a 100/0.5–1.3 oil-immersion objective.
10
Antibiotic Effects on Bacterial Viability
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Epifluorescence microscopy was used to observe the population-wise behavior of Evo3A and E. coli K-12 upon antibiotic treatment and resuscitation. Five hundred microliters of the cells being treated with antibiotic were taken, centrifuged, and resuspended in 0.85% NaCl. Cultures were stained with the LIVE/DEAD BacLight bacterial viability kit (Molecular Probes) according to the manufacturer’s standard protocol. A 1.5-μl amount of dye mixture containing SYTO 9 (1.67 mM) and propidium iodide (10 mM) was added to the 500-μl culture and incubated in the dark for 10 min. Stained cells were viewed with a fluorescence microscope (Eclipse Ni-U upright microscope) with appropriate filter sets. Images were captured with Nikon DS-Fi3 and associated software (NIS-Elements Ver. 5.00).
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