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Anti ifn γ b27

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Anti-IFN-γ (B27) is a monoclonal antibody that binds to and neutralizes the cytokine interferon-gamma (IFN-γ). It is commonly used as a research tool in immunology and cell biology studies.

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Lab products found in correlation

Anti tcr vγ9 immu360, by Beckman Coulter (2 mentions) Anti tnf α 188, by Beckman Coulter (2 mentions) Brefeldin a, by Merck Group (2 mentions) Facscanto 2, by BD (2 mentions) Foetal calf serum, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Lymphoprep, by Axis-Shield (1 mentions) Clariostar microplate reader, by BMG Labtech (1 mentions) Etanercept enbrel, by Amgen (1 mentions) Zometa, by Novartis (1 mentions) Anti ntb a nt7, by BioLegend (1 mentions) Anti nkg2d 1d11, by BioLegend (1 mentions) Anti nkp30 p30 15, by BioLegend (1 mentions) Anti dnam 1, by R&D Systems (1 mentions) Anti tcr vα7.2 3c10, by BioLegend (1 mentions)

Close spelling variants of this product

IFN-γ (422 citations) Anti-IFN-γ (149 citations) PE/Cy7-conjugated anti-IFN-γ (clone B27, (2 citations)

4 protocols using anti ifn γ b27

1

Zoledronate-Induced TNF-α Production in PBMCs

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The culture medium was RPMI‐1640 supplemented with 2 mml‐glutamine, 1% sodium pyruvate, 50 μg/ml penicillin/streptomycin and 10% foetal calf serum (Invitrogen, Paisley, UK). Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood of healthy volunteers using Lymphoprep (Axis‐Shield, Dundee, UK) and cultured with and without 10 µm zoledronate (Zometa; Novartis, Basel, Switzerland) for 16 hr; a combination of 10 ng/ml recombinant IFN‐γ and 20 ng/ml recombinant TNF‐α (both Miltenyi, Woking, UK) was used as positive control. For blocking experiments, anti‐IFN‐γ (B27; Biolegend, London, UK) and sTNFR p75‐IgG1 fusion protein (etanercept/Enbrel; Amgen, Cambridge, UK) were used at 10 μg/ml each. Cell culture supernatants were analysed in duplicate on a CLARIOstar microplate reader (BMG Labtech, Aylesbury, UK), using a sandwich ELISA kit for the detection of TNF‐α (Invitrogen).
Raffray L., Burton R.J., Baker S.E., Morgan M.P, & Eberl M. (2019). Zoledronate rescues immunosuppressed monocytes in sepsis patients. Immunology, 159(1), 88-95.
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2

Evaluating iNK Cell Cytotoxicity

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iNK cells were incubated for 30 minutes at 37°C with the following monoclonal antibodies: anti-NKG2D (1D11) at 20 μg/ml, anti-NTB-A (NT7) at 5 μg/ml, anti-NKp30 (P30–15) at 10 μg/ml, anti-LFA-1 (HI111) at 10 μg/ml (all from Biolegend), and anti-DNAM-1 at 10 μg/ml (102511) (R&D Systems) alone or in various combinations. iNK cells were then co-cultured with OVCAR8 cells for 4 hours in B0 media and analyzed by flow cytometry for intracellular IFN-γ production using a fluorescently conjugated anti-IFN-γ (B27) (Biolegend) antibody.
Cichocki F., Bjordahl R., Gaidarova S., Mahmood S., Abujarour R., Wang H., Tuininga K., Felices M., Davis Z.B., Bendzick L., Clarke R., Stokely L., Rogers P., Ge M., Robinson M., Rezner B., Robbins D.L., Lee T.T., Kaufman D.S., Blazar B.R., Valamehr B, & Miller J.S. (2020). iPSC-derived NK cells maintain high cytotoxicity and enhance in vivo tumor control in concert with T cells and anti-PD-1 therapy. Science translational medicine, 12(568), eaaz5618.
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3

Characterization of Peritoneal T-Cell Subsets

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Cells were acquired on an eight-color FACSCanto II (BD Biosciences) and analyzed with FlowJo 10.1 (TreeStar), using monoclonal antibodies against CD3 (SK7), CD69 (FN50), CCR4 (1G1), CCR5 (2D7) and CCR6 (11A9) from BD Biosciences; anti-TCR-Vγ9 (Immu360) from Beckman Coulter; anti-CD161 (HP-3G10), CCR2 (K036C2) and anti-TCR-Vα7.2 (3C10) from Biolegend; together with appropriate isotype controls. Anti-mouse antibodies reactive beads were used to set compensation (Life Technologies). Intracellular cytokines were detected using anti-IFN-γ (B27; Biolegend) and anti-TNF-α (188; Beckman Coulter). For detection of intracellular cytokines, 10 µg/ml brefeldin A (Sigma) was added to cultures 5 hours prior to harvesting. Leukocyte populations were gated based on their appearance in side scatter and forward scatter area/height and exclusion of live/dead staining (fixable Aqua; Invitrogen). Unless stated otherwise, peritoneal γδ T-cells were defined as Vγ9+ CD3+ lymphocytes. Peritoneal MAIT cells were defined as Vα7.2+ CD161+ CD3+ lymphocytes; control stainings using MR1 tetramers as reference confirmed the validity of this approach (data not shown).
Liuzzi A.R., Kift-Morgan A., Lopez-Anton M., Friberg I.M., Zhang J., Brook A.C., Roberts G.W., Donovan K.L., Colmont C.S., Toleman M.A., Bowen T., Johnson D.W., Topley N., Moser B., Fraser D.J, & Eberl M. (2016). Unconventional Human T Cells Accumulate at the Site of Infection in Response to Microbial Ligands and Induce Local Tissue Remodeling. The Journal of Immunology Author Choice, 197(6), 2195-2207.
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4

Multiparametric Flow Cytometry Analysis of Peritoneal T Cells

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Cells were acquired on an eight-color FACSCanto II (BD Biosciences) and analyzed with FlowJo 10.1 (Tree Star), using mAbs against CD3 (SK7), CD69 (FN50), CCR4 (1G1), CCR5 (2D7), and CCR6 (11A9) from BD Biosciences; anti–TCR-Vγ9 (Immu360) from Beckman Coulter; and anti-CD161 (HP-3G10), CCR2 (K036C2), anti–TCR-Vα7.2 (3C10) (BioLegend), together with appropriate isotype controls. Anti-mouse beads were used to set compensation (Life Technologies). Intracellular cytokines were detected using anti–IFN-γ (B27; BioLegend) and anti–TNF-α (188; Beckman Coulter). For detection of intracellular cytokines, 10 μg/ml brefeldin A (Sigma-Aldrich) was added to cultures 5 h prior to harvesting. Leukocyte populations were gated based on their appearance in side scatter and forward scatter area/height and exclusion of live/dead staining (fixable Aqua; Invitrogen). Unless stated otherwise, peritoneal γδ T cells were defined as Vγ9+CD3+ lymphocytes. Peritoneal MAIT cells were defined as Vα7.2+CD161+CD3+ lymphocytes; control stainings using MR1 tetramers as reference confirmed the validity of this approach (data not shown).
Liuzzi A.R., Kift-Morgan A., Lopez-Anton M., Friberg I.M., Zhang J., Brook A.C., Roberts G.W., Donovan K.L., Colmont C.S., Toleman M.A., Bowen T., Johnson D.W., Topley N., Moser B., Fraser D.J, & Eberl M. (2016). Unconventional Human T Cells Accumulate at the Site of Infection in Response to Microbial Ligands and Induce Local Tissue Remodeling. The Journal of Immunology Author Choice, 197(6), 2195-2207.
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