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Ferric chloride hexahydrate

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Ferric chloride hexahydrate is a chemical compound with the formula FeCl3·6H2O. It is a crystalline solid that is soluble in water and other polar solvents. Ferric chloride hexahydrate is commonly used as a coagulant in water treatment, as a mordant in dyeing, and in various other industrial applications.

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255 protocols using ferric chloride hexahydrate

1

Extraction and Characterization of Medicinal Plant Oils

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All chemicals, i.e. as ferrous chloride tetrahydrate (FeCl2·4H2O ≥ 99%), ferric chloride hexahydrate (FeCl3·6H2O, 97%), ammonium hydroxide (25%), isopropanol and n-hexane were obtained from Aldrich Chemical Co. (Missouri, USA) and used without further purification. Saudi Arabia-based heavy crude oil was supplied from the Riyadh refinery unit: Aramco Co. (Riyadh, KSA) and the seawater was collected from the Arabian Gulf along the Saudi coast.
The aerial parts of M. aurea and O. baccatus were collected from the natural area of Rowdah Khuraim (Riyadh, KSA) during March 2016. A taxonomist in the herbarium division of King Saud University identified the species. The plant’s aerial parts were chopped into small pieces and air-dried under shade. After drying, the materials were separately soaked in n-hexane for 72 h thrice. The n-hexane extracts of M. aurea (MAE) and O. baccatus (OBE) were filtered and concentrated under reduced pressure using rotary evaporator.
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2

Synthesis of Multifunctional Fluorescent Nanoparticles

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Ferric chloride hexahydrate (FeCl3 · 6H2O, >99%), anhydrous sodium acetate (NaOAc), ethylene glycol (EG), cadmium chloride (CdCl2 · 2.5H2O), tellurium dioxide (TeO2, 99.99%), 3-mercaptopropionic acid (MPA, 99%), glucose, polyvinylpyrrolidone (PVP), FA, N-hydroxysuccinimide (NHS), and N-ethyl-N-(3-(dimethylamino) propyl) carbodiimide (EDC) were purchased from Aldrich (Wyoming, IL, USA). HeLa cells were supplied by Zhejiang University. Sodium hydroxide (NaOH) and aqueous ammonia solution (25 wt %) were analytical grade. The pure water was obtained from a Milli-Q synthesis system (Millipore, Billerica, MA, USA).
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3

Synthesis of Magnetic PEG-Functionalized Nanoparticles

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Ferric chloride hexahydrate (FeCl3⋅6H2O, 99% w/w), ferric sulfate heptahydrate (FeSO4⋅7H2O, 99% w/w), ammonia solution (NH3, 32% v/v), hydrochloric acid (HCl, 32% v/v), 3-aminopropyltrimethoxysaline (NH2(CH2)3-Si-(OCH3)3, APTS), methanol (99.9% v/v), ethanol (99.9% v/v), methyl acrylate (99.5% v/v), methoxy-PEG, and ethylenediamine (99% v/v) were used in the synthesis process; all materials were purchased from Sigma Aldrich (Hamburg, Germany).
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4

Biobased Adsorbent Synthesis and Characterization

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Leaves of E. globulus were collected
from precincts of the University of Agriculture, Faisalabad for the
synthesis of extract. Sugar cane bagasse was collected from a local
marketplace of Faisalabad for the preparation of biomass and biochar.
In this research, we employed specific chemicals, namely, ferric chloride
hexahydrate (FeCl3·6H2O), ferrous chloride
tetrahydrate (FeCl2·4H2O), and sodium hydroxide
(NaOH), which were procured from Aldrich Chemicals. To ensure the
purity of our reagents, we used sterile distilled water sourced from
the biochemistry laboratory, with a conductivity measurement of 1
μS/cm. All the chemicals used in this study met analytical grade
standards and did not require further purification. We meticulously
prepared our solutions using deionized water, and all dilutions were
carried out using double-distilled water.
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5

Synthesis of Magnetic Nanoparticles

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Ferric chloride hexahydrate (FeCl3 · 6H2O, >99%), anhydrous sodium acetate (NaOAc), ethylene glycol (EG), polyvinylpyrrolidone (PVP), bovine serum albumin (BSA), and chitosan (low molecular weight, Brookfield viscosity 20 cps) were purchased from Aldrich (St. Louis, MO, USA). The pure water was obtained from a Milli-Q synthesis system (Millipore, Billerica, MA, USA).
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6

Comprehensive Chemical Characterization

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All chemicals used in the present work were of analytical grade, and purchased from Sigma-Aldrich Co. LLC (St. Louis, Missouri, USA): methanol, trichloroacetic acid, Folin-Ciocalteu's phenol reagent, bovine serum albumin, tyrosine, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tripyridyl-s-triazine (TPTZ), ferric chloride hexahydrate, and 6-hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid (Trolox).
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7

Synthesis of Iron Oxide Nanoparticles

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Ferric chloride hexahydrate (FeCl3·6H2O), ferrous chloride tetrahydrate (FeCl2·4H2O), Calcium chloride dihydrate (CaCl2.2H2O), diammonium hydrogen phosphate ((NH4)2(HPO4)), Cetyl trimethylammonium bromide (CTAB), ammonium hydroxide (NH4OH) and the other regents all were purchased locally from Sigma-Aldrich Chemicals and used as received.
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8

Synthesis and Characterization of Nanoparticles

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We obtained all chemical reagents of 95% purity and above from Sigma-Aldrich (St. Louis, MO, USA) and used them without further purification. Specifically, we procured β-cyclodextrin, 1-adamantylamine, NHS-PEG-maleimide, dichloromethane, trimethylamine, NaOH, p-toluene sulfonyl chloride, acetonitrile, ammonium chloride, ethanol, ethylenediamine, chitin, LiCl, dimethylacetamide, acetone, methanol, and slide-a-lyzer cassettes. Ferrous chloride tetrahydrate (FeCl2·4H2O) and Ferric chloride hexahydrate (FeCl3·6H2O) were purchased from Sigma Aldrich (St Louis, USA), H3NO.HCl and NH4OH was purchased from Alfa Aesar, HCl and tetramethylammonium hydroxide pentahydrate (98%) were obtained from Acros Organics (Thermo Scientific, USA), HAuCl4·3H2O, NaOH and sodium citrate were obtained from Aldrich (St Louis, USA). MillQ water, purchased from Barnstead NANOpure (Thermo Scientific) system run at 18.2M resistance was used in the synthesis. Cy5 labeled antimiR-21 RNA-oligo and miR-100 RNA-oligo were synthesized from Protein and Nucleic acid facility at Stanford (PAN, Stanford). Cell culture plates, FBS, penicillin, streptomycin, sodium bicarbonate, cell culture medium and phosphate-buffered saline (PBS) were purchased from GIBCO BRL (Frederick, MD).
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9

Enzyme-Catalyzed Nanoparticle Synthesis

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Ferric chloride hexahydrate (FeCl3·6H2O), ethylene glycol (EG), diethylene glycol (DEG), Acid Phosphatase (AP, MW = 10 kDa, EC 3.1.3.2), Superoxide Dismutase (SOD, ≥ 3000 U mg−1, EC 1.15.1.1) and Chloroperoxidase (CPO, ≥ 3000 U mL−1, EC 1.11.1.10) were purchased from Sigma-Aldrich. Sodium acetate (CH3COONa, NaOAc), Poly (vinylpyrrolidone) (PVP, K30), Pyrogallol, Succinic anhydride and 3-aminopropyltriethoxysilane (APTES) was purchased from Aladdin. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) were purchased from Energy Chemical, Fmoc-Tyr(H2PO3)-OH was purchased from GL Biochem. Tris·HCl buffer was purchased from Beijing Solarbio Science & Technology. Singlet Oxygen Sensor Green (SOSG) was purchased from Thermofisher. The aminophenyl fluorescein (APF) as the HClO detector were purchased from Shanghai Maokangbio. Enhanced BCA Protein Assay Kit was purchased from Beyotime Biotechnology. All materials were used without further purification.
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10

Comprehensive Antioxidant Capacity Assay

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The following reagent grade or greater chemicals were used: concentrated hydrochloric acid, ferric chloride hexahydrate, trolox, and 2,4,6‐tris(2‐pyridyl)‐s‐triazine (TPTZ) from Sigma Aldrich; ACS certified grade ferrous sulfate heptahydrate, glacial acetic acid, molecular biology grade ethanol (200 proof), and DPPH from Fisher Scientific; sodium acetate trihydrate (≥99%) from Avantor; gallic acid monohydrate from Acros Organics; Folin‐Ciocalteu reagent from Merck; sodium carbonate monohydrate from J. T. Baker Chemical; and ultrapure water produced on‐site. Nitrogen, 99.998% and helium, 99.999% were from Praxair. Chemicals were used without further purification. All chemicals originated from the United States.
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