α tcrβ fitc

Manufactured by BD

The α-TCRβ-FITC is a fluorescently-labeled antibody that binds to the beta chain of the T cell receptor (TCR) complex. It is used for the identification and characterization of T cells in flow cytometry applications.

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Lab products found in correlation

α b220 pe, by BD (1 mentions) α igm fitc, by BD (1 mentions) Advia 2120 analyser, by Siemens (1 mentions) αcd8 pe, by BD (1 mentions) α cd4 apc, by BD (1 mentions) α igd fitc, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) α cd11b pe cy7, by BioLegend (1 mentions) Anti γδtcr pe, by Thermo Fisher Scientific (1 mentions) αcd4 fitc, by BD (1 mentions) αcd3 pb, by BioLegend (1 mentions) αcd3 bv421, by BioLegend (1 mentions) αcd11c apc, by BioLegend (1 mentions) Lsr 2, by BD (1 mentions)

2 protocols using α tcrβ fitc

Immune Cell Profiling in Healthy Mice

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Healthy mice were euthanased at 28–30 days of age; their spleen, lymph nodes and thymus were collected and weighed; bone marrow (both femurs) and peripheral blood were also collected. Peripheral blood cell counts were enumerated using an ADVIA 2120 analyser (Siemens). Red blood cells were removed using 0.168 M ammonium chloride. Single cell suspensions were prepared from haemopoietic tissues and cell counts enumerated on a CASY Cell Counter (Scharfe System GmbH, Reutlingen, Germany). Cell composition was determined by immunostaining and flow cytometry (LSR II flow cytometer, BD Biosciences), using FlowJo software and the following fluorochrome-labelled surface marker-specific monoclonal antibodies produced in house: α-CD8-PE (clone YTS169); α-CD4-APC (clone H129.19); α-TCRβ-FITC (clone H57–597); α-B220-PE (clone RA3–6B2); α-IgM-FITC (clone 5.1); α-IgD-FITC (clone 11–26 C); α-IgD-PE; α-CD43-APC (clone S7); α-Mac1-PE (clone M1/70); α-Gr1-APC (clone RB6–8C5); α-Thy1-PE (clone T24/31); α-Ter119-APC.

Mensink M., Anstee N.S., Robati M., Schenk R.L., Herold M.J., Cory S, & Vandenberg C.J. (2018). Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells. Cell Death and Differentiation, 25(4), 795-806.

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Murine T Cell Immunophenotyping Protocol

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Anti-γδTCR-PE, αTCRβ-APC-eFluor780, αCD11c-FITC, αCD45.1-BV421, αSlamf4-PE, αSlamf4-FITC and αGranzymeB-FITC (eBioscience, San Diego, CA); αCD4-FITC and αTCRβ-FITC (BD Biosciences, San Jose, CA). αCD3-PB, αCD3-BV421, αCD8α-APC, αCD8α-PE-Cy7, αCD8β-PE, αCD8β-PerCP/Cy5.5, αCD4-BV650, αSlamf2-PE, αCD45.2-AlexaFluor 647, αCD45.2 FITC, αCD11b-PECy7, αCD11c-APC, αI-A/I-E-PB, αCCR9-PE, and αα4β7-APC were purchased from Biolegend (San Diego, CA). Data were collected using a BD LSRII and analyzed with FlowJo software (Tree Star, Ashland, OR).

O’Keeffe M.S., Song J.H., Liao G., De Calisto J., Halibozek P.J., Mora J.R., Bhan A.K., Wang N., Reinecker H.C, & Terhorst C. (2015). SLAMF4 is a Negative Regulator of Expansion of Cytotoxic Intra-epithelial CD8+ T Cells That Maintains Homeostasis in the Small Intestine. Gastroenterology, 148(5), 991-1001.e4.

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