2 6 di tert butyl 4 methylphenol

Manufactured by Merck Group

Sourced in Germany, United States

2,6-di-tert-butyl-4-methylphenol is a chemical compound used as an antioxidant in various industrial applications. It is a white crystalline solid with a characteristic phenol odor. The compound is known for its ability to inhibit oxidation and prevent degradation of materials.

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Lab products found in correlation

52 protocols using 2 6 di tert butyl 4 methylphenol

Lipid Extraction and Mass Spec Analysis

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Each sample (700 μL) was mixed with 800 μL 1 N HCl:CH₃OH 1:8 (v/v), 900 μL CHCl₃, 200 μg/mL of the antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT; Sigma Aldrich), and 3 μL of SPLASH^® LIPIDOMIX^® Mass Spec Standard (#330707, Avanti Polar Lipids, Alabaster, AL, USA). After vortexing and centrifugation, the organic fraction was evaporated using a Savant Speedvac spd111v (Thermo Fisher Scientific) at room temperature and the remaining lipid pellet was stored at—20 °C under argon atmosphere.

Fikatas A., Dehairs J., Noppen S., Doijen J., Vanderhoydonc F., Meyen E., Swinnen J.V., Pannecouque C, & Schols D. (2021). Deciphering the Role of Extracellular Vesicles Derived from ZIKV-Infected hcMEC/D3 Cells on the Blood–Brain Barrier System. Viruses, 13(12), 2363.

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Targeted MS analysis of lysophospholipids

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Lysophospholipids were analyzed by a targeted multiple reaction monitoring–based mass spectrometry method. Lipids were extracted by homogenizing cells in 1 N HCl/CH₃OH (1:8 [vol/vol]), CHCl₃, and 200 μg/ml antioxidant 2,6-di-tert-butyl-4-methylphenol (Sigma-Aldrich). Lysophospholipid standards were added based on the amount of DNA in the original sample (LPC 13:0 [31.98 nmol/mg DNA], LPC 17:1 [29.025 nmol/mg DNA], LPE 13:0 [36.4547 nmol/mg DNA], LPE 17:1 [32.2193 nmol/mg DNA], lysophosphatidylserine [LPS] 13:0 [31.4162 nmol/mg DNA], LPS 17:1 [32.2193 nmol/mg DNA], lysophosphatidylinositol [LPI] 13:0 [27.3938 nmol/mg DNA], and LPI 17:1 [24.931 nmol/mg DNA]). The organic fractions were evaporated and reconstituted in CH₃OH/CHCl₃/NH₄OH (90:10:1.25 [vol/vol/vol]). Phospholipids were analyzed by electrospray ionization tandem mass spectrometry on a hybrid quadrupole linear ion trap mass spectrometer (4000 QTRAP system; Applied Biosystems) equipped with a robotic nanoflow/ion source (Advion Biosciences, Ithaca, NY). The system was operated in the multiple reaction monitoring mode for quantification of individual species. Data were corrected for ¹³C isotope effects.

Gijs H.L., Willemarck N., Vanderhoydonc F., Khan N.A., Dehairs J., Derua R., Waelkens E., Taketomi Y., Murakami M., Agostinis P., Annaert W, & Swinnen J.V. (2015). Primary cilium suppression by SREBP1c involves distortion of vesicular trafficking by PLA2G3. Molecular Biology of the Cell, 26(12), 2321-2332.

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Antioxidant Analysis of Botanical Compounds

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The reagents of analytical grade were purchased from Sigma/Aldrich (St. Louis, MO): β-carotene, 2,6-di-tert-butyl-4-methylphenol (BHT), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), Folin-Ciocalteu reagent, disodium fluorescein, 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH), chemical standards (gallic acid, rutin hydrate, caffeic acid, kaempferol, and catechin), 1-nitropyrene (1-NP), dimethyl sulfoxide (DMSO). HPLC grade organic reagents were from Baker Inc. (Philipsburg, PA).

Olivas-Quintero S., López-Angulo G., Montes-Avila J., Díaz-Camacho S.P., Vega-Aviña R., López-Valenzuela J.Á., Salazar-Salas N.Y, & Delgado-Vargas F. (2017). Chemical composition and biological activities of Helicteres vegae and Heliopsis sinaloensis. Pharmaceutical Biology, 55(1), 1473-1482.

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Antioxidant Capacity Evaluation Protocol

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Folin–Ciocalteu (FCR), sodium carbonate (Na₂CO₃), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS, 2,6-di-tert-butyl-4-methylphenol (BHT), butylated hydroxyanisole (BHA), α-amylase from Aspergillus oryzae Green Alternative powder, ≥150 units/mg protein (biuret), gallic acid, quercetin, and AlCl₃ were purchased from Sigma (Sigma-Aldrich, Taufkirchen, Germany). All the organic solvents and other chemicals used in the present study were of analytical grade and were obtained from Sigma-Aldrich (Sigma-Aldrich, Germany).

Benmohamed M., Guenane H., Messaoudi M., Zahnit W., Egbuna C., Sharifi-Rad M., Chouh A., Seghir B.B., Rebiai A., Boubekeur S., Azli T., Harrat M., Sawicka B., Atanassova M, & Yousfi M. (2023). Mineral Profile, Antioxidant, Anti-Inflammatory, Antibacterial, Anti-Urease and Anti-α-Amylase Activities of the Unripe Fruit Extracts of Pistacia atlantica. Molecules, 28(1), 349.

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Lipid Extraction and Phospholipid Analysis

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PDEs and cells were prepared in 800 µL PBS as described above. An aliquot of 100 µL was set to quantify DNA for normalization. Lipids were extracted from the remaining 700 µL using a modified Bligh-Dyer protocol by adding 800 µL CHCl₃, 900 µL 1N HCl:CH₃OH 1:8 (v/v) and 500 μg of the anti-oxidant 2,6-di-tert-butyl-4-methylphenol (Sigma). Following centrifugal phase separation, the lower organic fraction was collected, evaporated, and the lipid pellet stored under argon gas at −20 °C. Lipid pellets were reconstituted in diluent (CH₃OH:CHCl₃:NH₄OH; 90:10:1.25, v/v/v) according to DNA quantification (1 µL diluent / 1 µg DNA). Phospholipid species were analyzed by ESI-MS/MS on a hybrid quadrupole linear ion trap mass spectrometer (4000 QTRAP system; Applied Biosystems, Foster City, CA) equipped with a TriVersa robotic nanosource (Advion Biosciences) as described previously [23 (link)].

Kwan K.H., Burvenich I.J., Centenera M.M., Goh Y.W., Rigopoulos A., Dehairs J., Swinnen J.V., Raj G.V., Hoy A.J., Butler L.M., Scott A.M., White J.M, & Ackermann U. (2020). Synthesis and fluorine-18 radiolabeling of a phospholipid as a PET imaging agent for prostate cancer. Nuclear medicine and biology, 93, 37-45.

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Bioactive Compound Extraction and Evaluation

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The W. somnifera extract (aqueous extract, 20:1) was purchased from Sanyuan Longsheng Biotechnology Co.(China). Peptone, beef extract and yeast extract were obtained from Aoboxing Biotechnology Co., China. Glucose, monopotassium phosphate, Na-acetate, magnesium sulfate, ethanol, sodium nitrite, aluminum nitrate, ethyl acetate, and sodium hydroxide were purchased from Kermel Scientific Co. (China). Folin–Ciocalteu phenol reagent was obtained from Solarbio. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,6-Di-tert-butyl-4-methylphenol (BHT) were purchased from Sigma-Aldrich Inc. (Germany). Pyrogallic acid, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ascorbic acid and rutin were obtained from Aladin Industries Inc. (China).

Yu J., Geng Y., Xia H., Ma D., Liu C., Wu R., Wu J., You S, & Bi Y. (2022). LAB Fermentation Improves Production of Bioactive Compounds and Antioxidant Activity of Withania somnifera Extract and Its Metabolic Signatures as Revealed by LC-MS/MS. Journal of Microbiology and Biotechnology, 32(4), 473-483.

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Fabrication of Photosensitive Liquid Crystal Elastomers

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All chemicals were used without treatment, as received. The LCE precursor included RM257 (monomer, >98.0%, TCI), PETMP (crosslinker, >95.0%, Sigma-Aldrich), EDDET (flexible chain extender, >95.0%, Sigma-Aldrich), dipropylamine (thermal catalyst, 99.0%, Sigma-Aldrich), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure-2959, photocatalyst, 98.0%, Sigma-Aldrich), 5CB (nematic liquid crystal dopant, 98%, Sigma-Aldrich), 2,6-di-tert-butyl-4-methylphenol (thermal inhibitor, ≥99.0%, Sigma-Aldrich), 2-(N-ethyl-4-((4-nitrophenyl)diazenyl)anilino)ethyl prop-2-enoate (Disperse Red 1 acylate, photothermal dye, 95.0%, Sigma-Aldrich) and chloroform (solvent, ≥99.0%, Sigma-Aldrich). IP-S (Nanoscribe) was used as the inactive photoresist for fabricating the passive microstructures, and 2-propanol (≥99.0%, Sigma-Aldrich) was used as the solvent for the development.

Zhang M., Pal A., Lyu X., Wu Y, & Sitti M. (2024). Artificial-goosebump-driven microactuation. Nature Materials, 23(4), 560-569.

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Antioxidant Activity Evaluation

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Chemicals: 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH), 2,6-di-tert-butyl-4-methylphenol (BHT), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ascorbic acid, dimethyl sulfoxide (DMSO), potassium persulfate, and all reagents were purchased from Sigma (St. Louis, MO, USA), Fluka Chemie (Buchs, Switzerland), and Merck (Nottingham, UK).

El Abed N., Kaabi B., Smaali M.I., Chabbouh M., Habibi K., Mejri M., Marzouki M.N, & Ben Hadj Ahmed S. (2014). Chemical Composition, Antioxidant and Antimicrobial Activities of Thymus capitata Essential Oil with Its Preservative Effect against Listeria monocytogenes Inoculated in Minced Beef Meat. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 152487.

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Plasma Biomarkers Assessment in Clinical Study

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Venous blood samples were obtained from each subject after 12 h fasting and drawn into pyrogen-free blood collection tubes. Several aliquots of plasma were placed into sterile 1 mL screw-capped polypropylene vials containing the phenolic antioxidant 2,6-di-tert-butyl-4-methylphenol (10 mM, Sigma-Aldrich Co., St Louis, MO, USA) to avoid lipid peroxidation and stored at − 80 °C. The samples were frozen and thawed only once. PBMCs were isolated as previously described [21 (link)]. High sensitivity C-reactive protein (CRP) was evaluated using a commercially available high-sensitivity turbidimetric method (Syncron-PCR; Beckman Coulter, Brea, CA, USA). Plasma 8-isoprostane (8-iso) was measured by means of Cayman’s 8-iso ELISA kit following the manufacturer’s indications. Total glutathione (GSH) was measured by means of Abcam’s GSH/GSSG ratio detection kit.

Fratta Pasini A.M., Stranieri C., Ferrari M., Garbin U., Cazzoletti L., Mozzini C., Spelta F., Peserico D, & Cominacini L. (2020). Oxidative stress and Nrf2 expression in peripheral blood mononuclear cells derived from COPD patients: an observational longitudinal study. Respiratory Research, 21, 37.

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Resin Blend for Dental Composites

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A resin blend was formulated containing 50:30:20 mass% bis-phenol A diglycidyl dimethacrylate, urethane dimethacrylate and triethylene glycol dimethacrylate (Esstech, Essington, PA, USA). 2,2-dimethoxy-2-phenylacetophenone at 0.1 mass%, and 2,6-di-tert-butyl-4-methylphenol at 0.5 mass% (SigmaAldrich, St. Louis, MO, USA), were used as the initiator and inhibitor, respectively. This formulation was similar to prior studies evaluating the addition of thiourethane to composite. ^{[5 (link)]} Ytterbium Fluoride fillers with average size of 0.11 μm (YBF100) and 0.05 μm (YBF402), and radiopaque Barium glass fillers with 0.80 μm were used in the study, and all fillers (Sukgyung AT. Co. Ltda, Ansa City, Korea) were almost spherical with a refractive index of 1.54. Fillers silanized using thiourethane or methacrylate were added at 40 mass% to the resin matrix.

Faria-e-Silva A.L., dos Santos A., Girotto E.M, & Pfeifer C.S. (2019). Impact of thiourethane filler surface functionalization on composite properties. Journal of applied polymer science, 136(25), 47687.

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