Magnesium chloride hexahydrate

Manufactured by Avantor

Sourced in Germany, Belgium

Magnesium chloride hexahydrate is an inorganic compound with the chemical formula MgCl₂·6H₂O. It is a white, crystalline solid that is soluble in water.

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Lab products found in correlation

Sodium chloride (nacl), by Merck Group (3 mentions) Sodium hydroxide (naoh), by Merck Group (3 mentions) Magnesium sulfate heptahydrate, by Avantor (2 mentions) Peg1000, by Merck Group (2 mentions) N hexane, by Thermo Fisher Scientific (2 mentions) Sodium sulfate, by Merck Group (2 mentions) Calcium chloride dihydrate, by Merck Group (2 mentions) Potassium chloride (kcl), by Merck Group (2 mentions) Zinc perchlorate hexahydrate, by Merck Group (1 mentions) Ethyl alcohol, by Thermo Fisher Scientific (1 mentions) Nitric acid, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions) Cerium 3 chloride heptahydrate, by Merck Group (1 mentions) Neodymium 3 nitrate hexahydrate, by Merck Group (1 mentions) Calcium standard for icp, by Merck Group (1 mentions) L lysine, by Thermo Fisher Scientific (1 mentions) Denatured ethyl alcohol, by Thermo Fisher Scientific (1 mentions) Arsenazo 3, by Horiba (1 mentions) Lanthanum 3 nitrate hexahydrate, by Merck Group (1 mentions) Calcium chloride, by Merck Group (1 mentions)

10 protocols using magnesium chloride hexahydrate

Trace Metal Enrichment Membrane Fabrication

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Polyethersulfone (PES) microfiltration membranes (0.45 μm pore diameter, 47 mm diameter) were purchased from MilliporeSigma (Burlington, USA). Glycidyl methacrylate (≥97.0%), lanthanum(III) nitrate hexahydrate (99.999% trace metal basis), cerium(III) chloride heptahydrate (99.9% trace metal basis), neodymium(III) nitrate hexahydrate (99.9% trace metal basis), zinc perchlorate hexahydrate, calcium chloride (anhydrous, Redi-Dri, ≥97%), sodium standard for ICP (1,000 mg/L ± 2 mg/L), calcium standard for ICP (1,000 mg/L ± 2 mg/L), and magnesium standard for ICP (1,000 mg/L ± 2 mg/L) were purchased from Sigma-Aldrich. Magnesium chloride hexahydrate (99–102%, ACS grade) was purchased from VWR Life Science. L-lysine (98%, powder) was purchased from Alfa Aesar. Ethyl alcohol (absolute, 200 proof, ≥99.5%, ACS Reagent grade) was purchased from Acros Organics. Denatured Ethyl alcohol (88–91%), sodium chloride (≥99%, certified ACS grade, crystalline), nitric acid (68–70%, certified ACS plus grade), and hydrochloric acid (36.5–38%, certified ACS plus grade) were purchased from Fisher Chemical. Arsenazo (III) was purchased from Pointe Scientific. Sodium hydroxide (10 N, biotech reagent grade) was purchased from Avantor. Deionized water (DI water) was made from a RiOS-DI 3 water purification system (MilliporeSigma, Burlington, MA, USA).

Yu M., Renner J.N, & Duval C.E. (2020). A Lysine-Modified Polyethersulfone (PES) Membrane for the Recovery of Lanthanides. Frontiers in Chemistry, 8, 512.

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Polyelectrolyte Multilayer Synthesis

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Two PCs were purchased:
PDADMAC (M_w = <100 kDa, 20 wt % in
water) from Sigma-Aldrich (The Netherlands) and PAH (M_w = 150 kDa) purchased from TCI chemicals (Belgium). As
PA poly(sodium 4-styrene sulfonate) (PSS, M_w = 500–700 kDa, 20.4 wt % in water) from Tosoh Organic Chemical
Co. LTD (Japan) was used. NaOH, NaI, D₂O, DMSO, DMF, and
THF were obtained from Sigma-Aldrich (The Netherlands). NMP was supplied
by VIVoChem (The Netherlands).
Potassium chloride (KCl, M_w = 74.55 g/mol), magnesium sulfate heptahydrate
(MgSO₄·7H₂O, M_w = 246.47 g/mol), magnesium chloride hexahydrate (MgCl₂·6H₂O, M_w = 203.30 g/mol),
and ethanol absolute (>99.9% purity) were obtained from VWR Chemicals
(Belgium). Sodium sulfate decahydrate (Na₂SO₄·10H₂O, M_w = 322.20 g/mol)
was obtained from Acros Organics (Belgium). Sodium chloride (NaCl, M_w = 58.44 g/mol) was obtained from Nouryon (SanalP,
pharmaceutical grade) (The Netherlands). Polyethylene glycols (PEGs)
with different molecular weights (M_w =
200, 400, 600, 1500, and 4000 g/mol) were obtained from Sigma-Aldrich
(The Netherlands). All chemicals were used as supplied.

Scheepers D., Casimiro A., Borneman Z, & Nijmeijer K. (2023). Addressing Specific (Poly)ion Effects for Layer-by-Layer Membranes. ACS Applied Polymer Materials, 5(3), 2032-2042.

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Enzymatic Plant Cell Wall Dissociation

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Cellulase Onozuka R-10 (PhytoTechnology Laboratories, Shawnee Mission, KS, USA).

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Macerozyme R-10 (A mixture of Pectinase, Cellulase and Hemicellulase; PhytoTechnology Laboratories).

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2-(N-Morpholino)ethanesulfonic acid hydrate (MES, Sigma-Aldrich, St. Louis, MO, USA, Cat. no. M8250).

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D-Mannitol (Sigma-Aldrich, Cat. no. M1902).

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Polyethylene glycol 4000 (PEG, Sigma-Aldrich,Cat. no. 95904).

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Potassium chloride (KCl, Sigma-Aldrich, Cat. no. P3911).

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Calcium chloride dihydrate (CaCl₂ 2H₂O, Sigma-Aldrich,Cat. no. C7902).

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Sodium chloride (NaCl, EMD, Merck, Kenilworth, N.J., USA).

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Magnesium chloride hexa-hydrate (MgCl₂.6 H₂O, VWR, Missouri, TX, USA, BDH-0244,).

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DL-Dithiothreitol (DTT, Sigma-Aldrich, Cat no. D0632).

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Propidium iodide (PI, Sigma-Aldrich, Cat. no P4170).

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Fluorescein diacetate (FDA, Sigma-Aldrich, Cat. no. F7378).

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Acetone (Sigma-Aldrich, Cat. no. 650501).

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Bovine serum albumin (BSA, EMD, Merck).

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Calf serum (Gibco, Thermo Fisher Scientific, Waltham, MA USA).

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Phosphate buffered saline (PBS, Gibco, Thermo Fisher Scientific).

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QIAprep Spin Miniprep Kit (QIAGEN, Germantown, MD, Cat. no. 27104).

Cheng N, & Nakata P.A. (2020). Development of a rapid and efficient protoplast isolation and transfection method for chickpea (Cicer arietinum). MethodsX, 7, 101025.

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Functionalized Polymeric Membranes Synthesis

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1,3,5-Tris(bromomethyl)benzene
(tBrMeB, 97%), p-phenylenediamine (PPD, 99%), m-phenylenediamine (MPD, 99%), poly(ethylene glycol)
(PEG, M_w = 400, 600 g mol^–1), sodium chloride (NaCl, ACS reagent, ≥99%, anhydrous, Redi-Dri,
free-flowing), sodium sulfate (Na₂SO₄, ACS reagent,
≥99%, anhydrous, granular, free-flowing, Redi-DriTM), and sodium
hydroxide (NaOH, BioXtra, ≥98%) were obtained from Sigma-Aldrich.
Magnesium sulfate heptahydrate (MgSO₄, ACS reagent. Ph.
Eur.), nitric acid (HNO₃, 1 mol L^–1,
Titripur), and PEG 1000 (EMPROVE ESSENTIAL) were supplied by Merck.
Magnesium chloride hexahydrate (MgCl₂, 99.0–101.0%
AnalaR NORMAPUR ACS Reag.) was purchased from VWR Chemicals (Netherlands). n-Hexane (anhydrous, over molecular sieves) was acquired
from Alfa Aesar (Germany). PEG 200 was obtained from Fluka (Germany).
Hydrophilized poly(ether sulfone) (PES) ultrafiltration membranes
with a molecular weight cutoff (MWCO) of 30 kDa were obtained from
Microdyn-Nadir (UH 030, Germany) and were used as support membrane.
According to the specifications and a previously reported study,^{31 (link)} the PES ultrafiltration support membranes are
stable in solutions with pH ranging from 0 to 14.

Asadi Tashvigh A., Elshof M.G, & Benes N.E. (2021). Development of Thin-Film Composite Membranes for Nanofiltration at Extreme pH. ACS Applied Polymer Materials, 3(11), 5912-5919.

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Bacterial Mutagenicity Assay Reagents

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All chemicals used in this study were of analytical grade. The NADP and glucose-6-phosphate used were obtained from Roche Biochem (Stockholm, Sweden). Aroclor-induced S9 from rat liver was purchased from Trinova Biochem (Giessen, Germany). Histidine, potassium chloride, magnesium sulfate, potassium phosphate dibasic anhydrous and sodium ammonium phosphate were purchased from Merck AG (Darmstadt, Germany). Magnesium chloride hexahydrate and citric acid monohydrate were acquired from VWR international (Leuven, Belgium). Biotin, tryptophan, methylcellulose (MC), dimethyl sulfoxide (DMSO), benzo[a]pyrene, 2-aminoanthracene, sodium azide, estradiol, bisphenol A, progesterone and testosterone were purchased from Sigma-Aldrich (Steinheim, Germany). D-Luciferin was obtained from Biotherma (Handen, Sweden). Yeast nitrogen base medium without amino acids was obtained from Becton Dickinson (Franklin Lakes, NJ, USA).

Omoruyi I.M., Ahamioje D, & Pohjanvirta R. (2014). Dietary Exposure of Nigerians to Mutagens and Estrogen-Like Chemicals. International Journal of Environmental Research and Public Health, 11(8), 8347-8367.

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Antioxidant and Cholinesterase Inhibition Assays

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Reagents for antioxidant activities and cholinesterase inhibition assays were from Sigma-Aldrich (St. Louis, MO, USA and Steinheim, Germany), except magnesium chloride hexahydrate, which was bought from VWR (Leuven, Belgium), sodium chloride from Fisher Scientific (Fair Lawn, NJ, USA), sodium nitroprusside dihydrate from Fluka, and sodium carbonate from Merck. Hydrogen peroxide 35.5% w/w was purchased from Labchem (Zelienople, PA, USA). Methanol Chromasolv for HPLC was from Riedel-de Haën (Seelze, Germany), and formic acid was from Carlo Erba (Val de Reuil, France). The standards used for HPLC analyses were acquired from Sigma-Aldrich (4-O-caffeoylquinic acid, quercetin-3-O-rutinoside hydrate, protocatechuic acid, ferulic acid, and p-coumaric acid), Fluka (caffeic acid), Alfa Aesar (5-O-caffeoylquinic acid), and Extrasynthèse (Genay, France): 3-O-caffeoylquinic acid, apigenin-8-C-glucoside, apigenin-6-C-glucoside, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, kaempferol-3-O-rutinoside, and tiliroside. Ultrapure water (with resistivities of 18.2 MΩ/cm) was obtained using a Milli-Q water purification system from Millipore (Molsheim, France). Standard solutions of Pb and Cd, Atomic Absorption standard with 4% HNO₃, were purchased from SPC Science (Quebec, Canada). Suprapur nitric acid 65% (v/v) and NH₄H₂PO₄ (Suprapur) were from Merck (Darmstad, Germany).

Rodrigues J.F., Soares C., Moreira M.M., Ramalhosa M.J., Duarte N.F., Delerue-Matos C, & Grosso C. (2023). Moringa oleifera Lam. Commercial Beverages: A Multifaceted Investigation of Consumer Perceptions, Sensory Analysis, and Bioactive Properties. Foods, 12(11), 2253.

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Characterization of PEG-based Nanofiltration Membranes

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CC (99%),
sodium hydroxide (NaOH, puriss. p.a., ACS reagent ≥98%), sodium
chloride (NaCl, ≥99%), sodium dodecyl sulfate (SDS, ACS reagent
≥99.0%), and poly(ethylene glycol) (PEG) with molecular weights
of 400, 600, and 1500 g mol^–1 were obtained from
Sigma Aldrich (Germany).
Sodium sulfate (Na₂SO₄, anhydrous, for analysis EMSURE ACS), magnesium sulfate heptahydrate
(MgSO₄, for analysis EMSURE ACS), and PEG 1000 (EMPROVE
ESSENTIAL) were obtained from Merck Millipore (Germany).
Magnesium
chloride hexahydrate (MgCl₂, 99.0–101.0%,
AnalaR NORMAPUR ACS Reag.) was purchased from VWR chemicals (Netherlands). n-Hexane (anhydrous, over molecular sieves) was acquired
from Alfa Aesar (Germany). 1,1,1-Tris(4-hydroxyphenyl)ethane (TPE,
>98.0%) was acquired from TCI (Belgium). PEG 200 was obtained from
Fluka (Germany).
Milli-Q water was used to prepare all aqueous
solutions. All chemicals
were used as received.
Hydrophilized polyethersulfone (PES)
ultrafiltration (UF) membranes
with a MWCO of 30 kDa were purchased from Microdyn-Nadir (UH030, Germany)
and used as the support. According to the supplier and earlier research,
these supports are stable in the pH range 0–14.^{11 (link)}

Elshof M.G., Maaskant E., Hempenius M.A, & Benes N.E. (2021). Poly(aryl cyanurate)-Based Thin-Film Composite Nanofiltration Membranes. ACS Applied Polymer Materials, 3(5), 2385-2392.

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Antibacterial Cellulose Paper Testing

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Norfloxacin, 95%, was purchased from abcr GmbH (Karlsruhe, Germany). Commercially available, cellulose-based paper (Soft & Sicher, dm-drogerie markt GmbH + Co. KG, Karlsruhe, Germany) was utilised as the paper matrix. Sucrose, peptone, beef extract, sodium chloride and potassium chloride were acquired from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). Agar was purchased from Sigma-Aldrich Pty Ltd. (Darmstadt, Germany). Magnesium sulfate heptahydrate, magnesium chloride hexahydrate, calcium chloride dihydrate, sodium hydrogen carbonate, di-potassium hydrogen phosphate and di-sodium hydrogen phosphate were acquired from VWR International GmbH (Darmstadt, Germany). Aliivibrio fischeri (A. fischeri) bacterial strain (ATCC 7744/ NCMB 1281) was obtained from Dr. G. Schuchardt^® (Göttingen, Germany). Purified water was freshly obtained from a PURELAB Flex 2 (ELGA LabWater, Veolia Water Technologies GmbH, Celle, Germany).

Abdelkader A., Nallbati L, & Keck C.M. (2023). Improving the Bioactivity of Norfloxacin with Tablets Made from Paper. Pharmaceutics, 15(2), 375.

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Liposome Preparation and Characterization

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DOPC was obtained from Avanti Polar Lipids. MDM with block molecular weights of 900-4800-900 Da and M_w/M_n of 1.23, and PB-PEO with block molecular weights of 2500-900 Da and M_w/M_n of 1.09 were obtained from Polymer Source Inc. 5(6)-CF, propionic acid, and butyric acid were obtained from Acros. M Ethanol, 1-propanol, boric acid, magnesium sulfate heptahydrate, magnesium chloride hexahydrate, and hydrochloric acid were obtained from J.T. Baker. Ethanol, 1-butanol, 1,2-ethanediol, 1,3-propanediol, 1,4-butanediol, 1,5-pentanediol, erythritol, xylitol, mannitol, urea, sodium chloride, Hepes, and acetic acid were obtained from Sigma-Aldrich. Glycerol and tris were obtained from American Bioanalytical. Triton X-100 was obtained from Thermo Fisher Scientific. Sodium hydroxide was obtained from Macron.

Werber J.R, & Elimelech M. (2018). Permselectivity limits of biomimetic desalination membranes. Science Advances, 4(6), eaar8266.

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Electrolyte Preparation for TRPV1 Channel Excitation

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Sodium chloride (VWR, Radnor, PA); potassium chloride (Macron Chemicals, Phillipsburg, NJ); magnesium chloride hexahydrate (J. T. Baker, Phillipsburg, NJ); calcium chloride dihydrate (Sigma-Aldrich, St. Louis, MO); ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich); ethylene glycol-bis(2-aminoethylether)-N, N, N′, N′-tetraacetic acid (EGTA) (Sigma-Aldrich) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) (Sigma-Aldrich) were used to prepare the electrolyte solutions. Capsaicin (purity > 95%, Cayman Chemical, Ann Arbor, MI) was dissolved in ethanol and stored at −20°C prior to use. On the day of experiment, the ethanol solvent was removed and the Capsaicin was dissolved in aqueous recording buffers, and used for TRPV1 channel excitations. All chemicals were used as received without further purification. Hydrochloric acid (EMD Millipore, Billerica, MA) and aqueous solutions of potassium hydroxide (Mallinckrodt, St. Louis, MO) and sodium hydroxide (EMD Millipore) were used for buffer pH adjustment. All aqueous solutions were prepared with deionized water (resistivity = 18 MΩ · cm at 25°C, EMD Millipore) and were filtered with 0.22 μm PVDF filter membranes (EMD Millipore) prior to use.

Shi W., Zeng Y., Zhu C., Xiao Y., Cummins T.R., Hou J, & Baker L.A. (2017). Characterization of Membrane Patch (MP) - Ion Channel Probes (ICPs) for Scanning Ion Conductance Microscopy. Small (Weinheim an der Bergstrasse, Germany), 14(18), e1702945.

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