Hsp60 sc 1052

Manufactured by Santa Cruz Biotechnology

HSP60 (sc-1052) is an antibody product offered by Santa Cruz Biotechnology. It is a monoclonal antibody that specifically recognizes the heat shock protein 60 (HSP60) antigen. HSP60 is a mitochondrial chaperonin involved in the folding and transport of proteins.

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Lab products found in correlation

Lsm 710, by Zeiss (1 mentions) Zen 2009, by Zeiss (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Bx51 microscope, by Olympus (1 mentions) Dp72 ccd, by Olympus (1 mentions)

Close spelling variants of this product

HSP60 (51 citations) Sc-1052 (6 citations) Goat anti-hsp60 (sc-1052; (2 citations)

2 protocols using hsp60 sc 1052

Visualizing Mitochondria and Peroxisomes in Cells

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MEFs expressing mito-DsRed were grown in DMEM containing 10% bovine calf serum. Peroxisomes were visualized by staining for Pex14 (rabbit; 10594–1-AP; Protein Tech) followed by a secondary antibody labeled with Alexa Fluor 488. Cells with any peroxisomes ≥2 µm long were scored as having tubular peroxisomes. Wild-type and Mff patient fibroblasts were provided by F. Alkuraya (Alfaisal University, Riyadh, Saudi Arabia).
For LC3 (rabbit; 2775; Cell Signaling Technology), P62 (rabbit; PM045; MBL), and PEX14 (rabbit; 10594–1-AP; Protein Tech) immunofluorescence, 10-µm cryosections of formalin-perfused, OCT-embedded heart was immunostained with the relevant antibodies and an Alexa Fluor 546–labeled donkey anti-rabbit (Life Technologies) secondary. HSP60 (sc-1052; Santa Cruz Biotechnology, Inc.) was visualized with Alexa Fluor 488–labeled donkey anti-goat.
Images were acquired using Zen 2009 software (Carl Zeiss) on a confocal microscope (LSM710; Carl Zeiss) at RT. A Plan-Apochromat 63×/1.4 oil objective was used. Photoshop was used only to change whole-image brightness/contrast and to crop.

Chen H., Ren S., Clish C., Jain M., Mootha V., McCaffery J.M, & Chan D.C. (2015). Titration of mitochondrial fusion rescues Mff-deficient cardiomyopathy. The Journal of Cell Biology, 211(4), 795-805.

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Immunohistochemical and Immunofluorescence Staining

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Paraffin-embedded tissue sections were dewaxed, rehydrated, and treated by 3% hydrogen peroxide in methanol for 30 min. Tissue sections were then incubated in blocking solution (5% normal goat serum and 0.3% Triton-X 100 in PBS) followed by incubating with HSP60 (SC-1052, Santa Cruz, CA) overnight at 4°C. The sections were rinsed and incubated with biotinylated donkey anti-goat immunoglobulin G (IgG) and then reacted to Vector Elite ABC in which the color was developed with diaminobenzidine. Images were taken by using the Olympus DP72 CCD attached to an Olympus BX51 microscope with DP controller. For the immunofluorescence staining, antibodies of cleaved caspase-3 (Cell Signaling 9961), CD4 (MAB554), and CD8α (MAB116) were used for incubating samples overnight in blocking solution at 4°C. Alexa Fluor 488 conjugated goat anti-rabbit IgG (H+L) or goat anti-mouse IgG (H+L) secondary antibodies (Invitrogen) were used instead. The mounting media containing DAPI (Invitrogen) were used for counterstaining cell nucleus.

Chen T.H., Liu S.W., Chen M.R., Cho K.H., Chen T.Y., Chu P.H., Kao Y.Y., Hsu C.H, & Lin K.M. (2015). Neonatal Death and Heart Failure in Mouse with Transgenic HSP60 Expression. BioMed Research International, 2015, 539805.

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