Hrp conjugated goat anti mouse or goat anti rabbit secondary antibodies

Cell lysates were prepared in lysis buffer and quantified by Bradford assay. Equivalent amounts of each sample were resolved by SDS-PAGE and Western blotted with the following antibodies: Rabbit anti-NCL (Abcam), Mouse anti-NCL (Santa Cruz), Rabbit anti-eIF4H (Cell signaling). Rabbit anti-Flag (Sigma), Mouse anti hnRNPC1/C2 (Abcam), Rabbit anti-H3 (Cell Signaling), Rabbit anti-Xrn1 (Sigma), Mouse anti-Strep (Qiagen). Primary antibodies were followed by HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Southern Biotechnology, 1:5000).

Cell lysates were prepared in lysis buffer and quantified by Bradford assay. Equivalent amounts of each sample were resolved by SDS-PAGE and Western blotted with the following anti-MHV68 primary antibodies: hybridoma supernatants T1A1 anti-gp150, MG-2C10 anti-gB, and 9c7 anti-gp70, diluted 1∶10 (kindly provided by Phillip Stevenson, University of Cambridge); anti-M9, anti-ORF26, and anti-ORF45, diluted 1∶500 (kindly provided by Ren Sun, UCLA); anti-ORF72, diluted 1∶500 (kindly provided by Linda van Dyk, University of Colorado, Denver); anti-ORF49, diluted 1∶500 (kindly provided by Moon Jung Song, Korea University). Primary antibodies were followed by HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Southern Biotechnology, 1∶5000).

Lysates were resolved by SDS-PAGE and western blotted with the following antibodies at 1:1000 in TBST (Tris-buffered saline, 0.1% Tween 20): rabbit anti-EST1A/SMG6 (Abcam), rabbit anti-NCL (Abcam), rabbit anti-HuR (Millipore), rabbit anti-actin (Abcam), rabbit anti-IGF2BP1 (Abcam), rabbit anti-YTHDC2 (Abcam), rabbit anti-hnRNPU (Abcam), rabbit anti-ZC3HAV1 (Abcam), rabbit anti-hnRNPD (Abcam), rabbit anti-STAU1 (Pierce), mouse anti-NPM1 (Abcam), rabbit anti-GADD45 (Abcam), mouse anti-GAPDH (Abcam), mouse anti-ORF59 (Adv biotechnologies), or rabbit anti-K8.1 (PRF&L, inc). Primary antibody incubations were followed by HRP-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Southern Biotechnology, 1:5000).