2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid abts solution

PIC1 (IALILEPICCQERAA-dPEG24) was manufactured by PolyPeptide Group (San Diego, CA) to ≥ 95% purity verified by HPLC and mass spectrometry analysis. Lyophilized PIC1 was solubilized in normal saline with 0.01 M Na₂HPO₄ buffer to 15 mM. Pure MPO (MPO) was purchased from Lee Biosolutions (Maryland Heights, MO). TMB as a solution containing H₂O₂ was purchased from Thermo Fisher Scientific (Waltham, MA). 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) solution was purchased from Thermo Fisher Scientific. O-dianisidine and 4-aminobenzoic acid hydrazide (ABAH) were purchased from Sigma-Aldrich (St. Louis, MO).

PIC1 (IALILEPICCQERAA-dPEG24) was manufactured by PolyPeptide Group (San Diego, CA) to ≥95% purity as verified by HPLC and mass spectrometry analysis. Lyophilized PIC1 was solubilized in normal saline with 0.01 M Na₂HPO₄ buffer to 15 mM. dPEG24 was purchased from PolyPeptide Group and dissolved in the same buffer. Pure human methemoglobin (metHb), myoglobin from equine skeletal muscle, and L-cysteine were purchased from Sigma (St. Louis, MO). 3,3′,5,5′-Tetramethylbenzidine (TMB) as a solution containing H₂O₂ was purchased from Thermo Fisher Scientific (Waltham, MA). 4-Aminobenzoic acid hydrazide (ABAH), O-dianisidine, and NaOCl were purchased from Sigma-Aldrich (St. Louis, MO). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) solution was purchased from Thermo Fisher Scientific (Waltham, MA).