Imatinib was provided by Novartis Pharma AG (Basel, Switzerland). Antibodies for Western blotting, including caspase-9, caspase-3, cleaved caspase-3, PARP, phospho-c-Abl, phospho-Elk-1, phospho-cyclin-dependent kinase 1 (Cdk1) (Thr161), phospho-Cdk1 (Tyr15), phospho-ERK1/2, ERK1/2, phospho-Akt, Akt, phospho-STAT3, STAT3, Bcl-2, and Bcl-xL, were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies specific for c-Abl, multidrug resistance 1 (MDR1), α-tubulin, cyclin B1, Cdk1, Mcl-1, Bax, cytochrome c, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). An antiphosphospecific MPM2 monoclonal antibody was purchased from Upstate Biotechnology (Lake Placid, NY, USA).
Phospho elk 1
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-Elk-1 is an antibody reagent produced by Cell Signaling Technology that recognizes Elk-1 protein when phosphorylated at specific serine residues. It can be used to detect and quantify the phosphorylation state of Elk-1 in biological samples using techniques such as Western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Phospho akt, by Cell Signaling Technology (2 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Phospho c abl, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Mcl 1, by Santa Cruz Biotechnology (1 mentions)
Cyclin b1, by Santa Cruz Biotechnology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Phospho stat3, by Cell Signaling Technology (1 mentions)
Cytochrome c, by Santa Cruz Biotechnology (1 mentions)
Imatinib, by Novartis (1 mentions)
Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
Phospho raf1 ser259, by Cell Signaling Technology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
3 protocols using phospho elk 1
1
Imatinib Apoptosis Signaling Pathway Analysis
2
Western Blot Analysis of H-Ras Signaling
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Cells were lysed in buffer that contained 20 mM HEPES (pH 7.2), 1% Triton X-100, 10% glycerol, 400 mM NaCl, 10 μg/ml leupeptin, and 1 mM PMSF. Western blot analysis was performed according to standard procedures, as described previously (32) (link), using an antibody against either H-Ras (1 : 500; Oncogene), phospho-Raf1 (Ser259; 1 : 1,000; Cell Signaling), phospho-Erk1/2 (Thr202/Tyr204; 1 : 1000; Cell Signaling), phospho-RSK (Thr573; 1 : 1,000; Cell Signaling), phospho-Akt (Ser473; 1 : 1,000; Cell Signaling), Erk2 (1 : 5,000; Santa Cruz Biotechnology), phospho-Elk1 (Ser383; 1 : 1,000; Cell Signaling), MKP3 (1 : 5,000; Cell Signaling), or GAPDH (1 : 2,000; Santa Cruz Biotechnology).
3
Withaferin A Modulates MAPK Signaling in HepG2 and Huh7 Cells
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HepG2 cells (ATCC, Manassas, VA) derived from a human hepatoblastoma37 (link) and Huh7 cells derived from a well-differentiated HCC38 (link) were cultured in minimum essential medium (ATCC) and Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA), respectively. Withaferin A (WA) was purchased from Calbiochem EMD Millipore (Billerica, MA). Antibodies for phospho-ERK, phospho-ELK1, phospho-RSK, phospho-p38, ERK, ELK1, RSK, DR5, Cleaved Caspase 3, Bax, cleaved-PARP, total-PARP, PCNA and β-actin were purchased from Cell-Signaling Technology (Danvers, MA). U0126 was procured from Sigma-Aldrich (St. Louis, MO).
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