HER2 + breast cancer cell lines (BT474, SKBR3, MDA-MB-453), HER2- breast cancer cell line (MDA-MB-231), and CD4 + T-cell line (Jurkat) were obtained from ATCC (Manassas, VA, USA). BT474 cells were grown in improved minimal essential media (Invitrogen, Carlsbad, CA) with 10% FBS and 1% insulin. SKBR3 cells were grown in McCoy’s 5A media with 10% FBS and 2 mM L-glutamine. MDA-MB-453 cells were grown in Leibovitz’s L-15 media (Sigma, St. Louis, MO, USA) with 10% FBS. MDA-MB-231 cells were grown in Dulbecco’s minimal essential media (Gibco, Gaithersburg, MD, USA) with 10% FBS, 2 mM L-glutamine, and 1 mM sodium pyruvate. Jurkat T-cells were grown in RPMI-1640 media (Gibco, Gaithersburg, MD, USA) with 10% FBS and 2 mM L-glutamine. Co-culturing of cancer cells and T-cells were conducted by suspending cancer cells and T-cells in cancer cell’s respective growth medium prior to plating.
Leibovitz s l 15 media
Manufactured by Merck Group
Sourced in United States
Leibovitz's L-15 media is a cell culture medium developed by Albert Leibovitz. It is a balanced salt solution that provides the necessary nutrients and components for the growth and maintenance of various cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 media, by Thermo Fisher Scientific (2 mentions)
Mda mb 231, by Thermo Fisher Scientific (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Eagle s minimum essential media, by Merck Group (2 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Dulbecco s minimal essential media, by Thermo Fisher Scientific (1 mentions)
Mda mb 453, by Merck Group (1 mentions)
Sw1353, by American Type Culture Collection (1 mentions)
Crl 2522, by American Type Culture Collection (1 mentions)
Sw1353, by Merck Group (1 mentions)
Humidified incubator, by Thermo Fisher Scientific (1 mentions)
Dmem media, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
4 protocols using leibovitz s l 15 media
1
Breast Cancer and T-cell Co-culture
2
Cell line culture for arthritis and skin research
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Chondrosarcoma (SW1353, HTB-94) and normal dermal fibroblast (BJ, CRL-2522) cell lines were acquired from American Type Culture Collection. SW1353 and BJ cell lines were utilized for their relevance as a disease-related model for arthritis and skin manifestations, respectively [36 (link),37 (link)]. SW1353 cells were grown in Leibovitz’s L-15 media (Sigma), supplemented with 10% fetal bovine serum (Gibco, Paisley, UK), 2 mM l -glutamine (Gibco), and 100 IU/mL Penicillin/0.2 mg/mL streptomycin (Gibco) antibiotic cocktail, and incubated at +37 °C with 100% air. BJ cells were grown in Eagle’s minimum essential media (Sigma), with the above-mentioned supplements and an additional 1 mM sodium pyruvate (Gibco), and incubated at +37 °C, 5% CO2.
3
Skin and Arthritis Cell Models
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Normal dermal fibroblast cell line (BJ, CRL-2522) and chondrosarcoma cell line (SW1353, HTB-94) were purchased from American Type Culture Collection. Dermal fibroblasts have been previously used in investigations of borrelial infection (Georgilis et al., 1992 (link)). Furthermore, the use of chondrosarcoma cells in osteoarthritis research is well documented (Chen et al., 2017 (link); Liu et al., 2017 (link)). Therefore, these cell lines, BJ and SW1353, were utilized for their relevance as a disease-related model for skin manifestations and arthritis, respectively. The SW1353 cells were grown at +37°C with 100% air in Leibovitz’s L-15 media (Sigma), while the BJ cells were at +37°C, 5% CO2 in Eagle’s minimum essential media (Sigma) as instructed by the manufacturers. Both media were supplemented with 10% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), and 100 IU/ml penicillin/0.2 mg/ml streptomycin (Gibco) antibiotic cocktail. Sodium pyruvate (1 mM, Gibco) was also added to the BJ media.
4
Detailed Cell Culture Protocol for Breast and Thyroid Cancer
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HDACi drugs such as TSA [T8852], NaB [B5887], VPA [4543], SAHA [SML0061], TBA [SML0044] and all trans retinoic acid [R2625] were purchased from Sigma, USA. CI994 (1742-10, 50) was purchased from Biovision, CA, USA. Stock solutions (1M) of TSA, SAHA, CI994 and TBA were prepared in DMSO and of NaB and VPA were prepared in sterile MiliQ and stored at −20 °C. Receptor positive MCF-7 and Zr-75-1 BC cells (ATCC) were maintained in RPMI-1640 media (Gibco, Invitrogen, USA). Receptor negative MDA-MB-231 cells (ATCC) were maintained in Leibovitz’s L15 media (Sigma, USA) whereas MDA-MB-468 cells (a gift from Dr. M. Vaidya, ACTREC, India) were maintained in DMEM media (Gibco, Invitrogen, USA) supplemented with 10 mM HEPES. All the media contained 10% fetal bovine serum (FBS) (Gibco, Invitrogen, USA), 1% antibiotic-antimycotic solution (Gibco, Invitrogen, USA). Two thyroid cancer cell lines, NPA and ARO (gifted by Mr. A. Chakraborty, BARC, India), were maintained in IMDM (Gibco, Invitrogen, USA) containing 10% FBS and 0.075% gentamycin solution. All the cells were maintained at 37 °C in a humidified incubator (Thermo Scientific, Rockford, IL, USA) with 5% CO2 except for MDA-MB-231.
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