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Dodium citrate dehydrate

Manufactured by Merck Group

Dodium citrate dehydrate is a chemical compound used in various laboratory applications. It functions as a buffer, maintaining a specific pH level in solutions. The compound is typically used in biochemical and analytical procedures where pH control is essential.

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2 protocols using dodium citrate dehydrate

1

Synthesis and Characterization of Functionalized Nanoparticles

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TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). p-SCN-Bn-NOTA (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, hematoxylin staining solution, and Chelex 100 resin (50–100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-Aminopropyl)triethoxysilane (APS), Cetyltrimethylammonium chloride solution (CTAC, 25 wt %), copper(II) chloride (CuCl2), sodium sulfide nonahydrate (Na2S·9H2O) and dodium citrate dehydrate were purchased from Sigma-Aldrich (St. Louis, MO). AlexaFluor488- and Cy3-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, sodium chloride (NaCl), doxorubicin hydrochloride (DOX) were purchased from Fisher Scientific. SCM-PEG5k-Mal was obtained from Creative PEGworks. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
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2

Bioconjugation of TRC105 with NOTA

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TRC105 was provided by TRACON Pharmaceuticals Inc. (San Diego, CA). p-SCN-Bn-NOTA (i.e., 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7- triacetic acid) was acquired from Macrocyclics, Inc. (Dallas, TX). NHS-fluorescein, hematoxylin staining solution, and Chelex 100 resin (50~100 mesh), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-Aminopropyl)triethoxysilane (APS), Cetyltrimethylammonium chloride solution (CTAC, 25 wt%), copper(II) chloride (CuCl2), sodium sulfide nonahydrate (Na2S·9H2O) and dodium citrate dehydrate were purchased from Sigma-Aldrich (St. Louis, MO). AlexaFluor488- and Cy3-labeled secondary antibodies were purchased from Jackson Immunoresearch Laboratories, Inc. (West Grove, CA). PD-10 columns were purchased from GE Healthcare (Piscataway, NJ). Absolute ethanol, sodium chloride (NaCl), doxorubicin hydrochloride (DOX) were purchased from Fisher Scientific. SCM-PEG5k-Mal was obtained from Creative PEGworks. Water and all buffers were of Millipore grade and pretreated with Chelex 100 resin to ensure that the aqueous solution was free of heavy metals. All chemicals were used as received without further purification.
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