Anti-CD9 antibody [MEM-61] and anti-CD63 antibody [MEM-259] were purchased from Abcam. Recombinant protein G from Escherichia coli, ≥90% (protein G), anti-human IgG antibody (anti-IgG), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimidehydrochloride (EDC), N-hydroxyl-succinimide (NHS), and 11-mercaptoundecanoic acid (MUA) were all purchased from Sigma-Aldrich. ExoStdTM lyophilized exosome standard (human serum) (Exo) was bought from Biovision (Milpitas, USA). Deionized water was obtained from a Milli-Q purification system (18.2 mΩ at 25 °C). Phosphate-buffered saline (PBS, pH 7.4) and PBST (containing 0.05% Tween-20) were prepared in deionized water.
Mem 259
Manufactured by Abcam
MEM-259 is a monoclonal antibody that recognizes the CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5) protein. CEACAM5 is a cell surface glycoprotein that is often overexpressed in certain types of cancer cells.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using mem 259
1
Exosome Immunophenotyping Using Antibodies
2
Quantification of HBV DNA in Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each aliquot (200 μL at 3.0 × 104 viral DNA copies/mL) of Sup, EV-, and EV+ fractions from primary human hepatocytes was mixed with an equal volume of PBS containing 1% bovine serum albumin (BSA) and 5 μg of one of the following: anti-CD9 antibody (M-L13; Becton, Dickinson and Company, Franklin Lakes, NJ), anti-CD63 antibody (MEM-259; Abcam, Cambridge, UK), anti-CD81 antibody (JS-81; Becton, Dickinson and Company), or anti-hemagglutinin (HA) tag antibody (12CA5(2); BioVision, San Francisco, CA), or with diluted serum (1:300) from a normal (HBV-naive) or HBs-immunized mouse. The resulting mixture was incubated for 16 hours at 4°C. Each fluid + antibody mixture then was combined with 50 μL of a 50% slurry of protein G–Sepharose 4 Fast Flow (GE Healthcare, Chicago, IL) and incubated with rotation at 4°C for 2 hours. The mixtures were centrifuged at 2300 × g for 20 seconds at 4°C, and the supernatants were discarded. The pellets were washed twice with 1% BSA in 10% Dulbecco’s modified Eagle medium. The pellets then were resuspended in 10% Dulbecco’s modified Eagle medium containing 1% BSA and used for quantification analysis of HBV DNA.
3
Immunogold Labeling of CD63+ Microvesicles
4
Extracellular Vesicle Detection Assay
5
Immunogold Labeling of Microvesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microvesicle fractions resuspended in PBS obtained from ultracentrifugation of culture supernatants of CD4+ T cells after 72 h of stimulation were bound to glow-discharged formvar-coated grids contrasted with 1% uranyl acetate. Immunogold labeling of vesicles for CD63 was performed by staining with mouse anti-human CD63 mAb MEM-259 (Abcam), followed by goat anti-mouse IgG coupled to 10 nm gold (BB International). Samples were viewed with a FEI Tecnai G20 transmission electron microscope operated at 120 kV. Images were captured using an AMT XR60B (Deben) digital camera running Deben software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!