Mh7a cells

Manufactured by Jennio Biotech

Sourced in China

MH7A cells are a well-established murine myeloma cell line. They are commonly used for the production of monoclonal antibodies in hybridoma technology.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin, by Beyotime (1 mentions) Streptomycin, by Beyotime (1 mentions) Microplate reader, by Agilent Technologies (1 mentions)

4 protocols using mh7a cells

Culturing RASFs, NSFs, and MH7A Cells

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RASFs and NSFs were cultured in DMEM/high−glucose medium (Hyclone, Logan, UT, USA) containing 20% (v/v) fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 100 mg/mL of streptomycin, and 100 U/mL of penicillin (both from Beyotime, Shanghai, China) at 37 °C in a humidified atmosphere containing 95% air and 5% CO₂.
MH7A cells, a human rheumatoid arthritis synovial cell line, were obtained from Jennio Biotech Co., Ltd. (Guangzhou, China). The cells were incubated in DMEM/high-glucose medium (Hyclone, Logan, UT, USA) containing 10% (v/v) fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 100 mg/mL of streptomycin, and 100 U/mL of penicillin (both from Beyotime, Shanghai, China) at 37 °C in a humidified atmosphere containing 95% air and 5% CO₂.

Xu Y., Zai Z., Lu Z., Zhang T., Wang L., Qian X., Tao J., Peng X., Zhang Y, & Chen F. (2023). Circular RNA CircCDKN2B−AS_006 Promotes the Tumor-like Growth and Metastasis of Rheumatoid Arthritis Synovial Fibroblasts by Targeting the miR−1258/RUNX1 Axis. International Journal of Molecular Sciences, 24(6), 5880.

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Cytotoxicity Assay of MH7A Cells

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MH7A cells (Guangzhou Jennio Biotech Co., Ltd, P. R. China) were maintained in DMEM supplied with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin with a humidified atmosphere 5% CO₂ at 37 °C. Passages between 4 and 7 of MH7A cells were used in the current study. All the compounds and other agents were dissolved in DMSO as stock solutions. MH7A cells were seeded in 96-well culture plates (5 (link) × 10³ cell/well) for 24 h and then pre-treated or untreated with 15 μM tested compounds for 2 h, then incubated for another 24 h with or without stimulation of 10 ng/mL of TNF-α.

Luo Y.X., Gong X., Su Z.C., Mo J.F., Li D.L., Wu R.H., Jin J.W., Lang M., Wang J.P., Xu X.T, & Gan L.S. (2024). Isolation, structure modification, and anti-rheumatoid arthritis activity of isopimarane-type diterpenoids from Orthosiphon aristatus. Journal of Enzyme Inhibition and Medicinal Chemistry, 39(1), 2296355.

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Culture and Passage of MH7A Cells

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MH7A cells were obtained from Jennio Biotech Co., Ltd. (Guangzhou, China), and cultured in DMEM supplemented with 10% FBS, 100 U∙mL⁻¹ penicillin and 100 μg∙mL⁻¹ streptomycin at 37 °C in 5% CO₂ atmosphere. Cells were passaged every 3–4 days. Cells obtained from the 4th–10th passages were used in the experiment.

Qiu H., Sun S., Ma X., Cui C., Chen G., Liu Z., Li H, & Liu M. (2018). Jatrorrhizine Hydrochloride Suppresses Proliferation, Migration, and Secretion of Synoviocytes In Vitro and Ameliorates Rat Models of Rheumatoid Arthritis In Vivo. International Journal of Molecular Sciences, 19(5), 1514.

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Cell Viability Assay with MH7A Cells

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MH7A cells (Jennio Biotech Co., Ltd, China) were cultured as we reported previously. (Chen et al., 2022 (link)). For cell viability detection, MH7A cells were seeded in 96-well plates overnight before being stimulated with different dosages of compounds (2.5–20 μM) for 24 h. Subsequently, the treatment of the resulting cells was co-incubated with CCK-8 solution (10 μL/well; APExBIO Corporation) for 1 h. Finally, the absorbance of each well was tested through a microplate reader (450 nm; BioTek, United States).

Bao X., Wei J., Tao C., Bashir M.A., Zhang H.J., Bao B., Chen J, & Zhai H. (2023). Akuammiline alkaloid derivatives: divergent synthesis and effect on the proliferation of rheumatoid arthritis fibroblast-like synoviocytes. Frontiers in Chemistry, 11, 1179948.

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