The following pre-designed siRNAs were purchased from Dharmacon: AdipoR2, Non-Target, PPARA, PPARG, RXRA, S1PR1, S1PR2, S1PR3, SCD, Sphk1, Sphk2, SREBF1 and SREBF2 (catalog numbers in Supplementary Table 1 —see supplementary information file). Transfection of 25 nM siRNA was performed in complete media using Viromer Blue according to the manufacturer’s instructions 1X (Lipocalyx). Knockdown gene expression was verified by qPCR 48 h after transfection.
Non target
Manufactured by Horizon Discovery
Sourced in United States
Non-Target is a laboratory equipment product designed for use in gene editing applications. It functions as a control tool to assess off-target effects during the gene editing process. The core purpose of Non-Target is to provide researchers with a reliable means to evaluate the specificity and efficiency of their gene editing techniques.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using non target
1
Targeted Knockdown of Lipid Regulators
2
Transfection and CDK Protein Analysis
3
Transfection and CDK Protein Analysis
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Cells were reverse-transfected with either Non-Target, CDK4, or CDK6 siRNA (Dharmacon) using lipofectamine 3000 according to the manufacturer’s instructions. Protein expression was analyzed by western blot after 72 hours.
4
Inducible LSD1 Silencing in HMEC-1
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The silencing of lysine-specific demethylase 1 was performed in HMEC-1 using an lentivirial inducible shRNA system, Inducible Dharmacon TM TRIPZ TM (Dharmacon, Lafayette, U.S.) taking into account the manufacturer's instructions. Silencing efficiency was checked by four LSD1 shRNA sequences (#RHS4696-200767629, #RHS4696-200763212, #RHS4696-200695244, #RHS4696-200765304) purchased from Dharmacon, Lafayette, U.S. As a control for LSD1 DMT silencing, cells were transduced with an empty vector -nonTarget (non-silencing shRNA, #RHS4743; Dharmacon, Lafayette, U.S.). Upon addition of 1 µg/ ml doxycycline (DOX) to HMEC-1 to induce LSD1 silencing, expression of the targeted gene was estimated by quantitative PCR and Western blotting (WB).
5
siRNA Knockdown of Lipid Regulators
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The following pre-designed siRNAs were purchased from Dharmacon: AdipoR2, Non-Target, PPARA, PPARG, RXRA, S1PR1, S1PR2, S1PR2, SCD, Sphk1, Sphk2, SREBF1 and SREBF2. Transfection of 25 nM siRNA was performed in complete media using Viromer Blue according to the manufacturer's instructions 1X (Lipocalyx). Knockdown gene expression was verified by qPCR 48 h after transfection.
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