Openlab cds chemstation edition 2012

RP-HPLC method was used to determinate phenolic compounds by an Agilent 1260 series HPLC instrument (Agilent Technologies, Palo Alto, CA, USA) equipped with a quaternary pump (G1311B), degasser, column thermostat (G1316A), auto-sampler (G1329B) and diode array detector (G1315 B, DAD). Chromatographic separation was carried out according to Molinu et al. [20 (link)]. The column was a Zorbax Eclipse plus C18 (250 × 4.6 mm, 5 µm; Agilent). Column temperature was set to 30 °C and the flow rate was 0.8 mL min⁻¹. The injection volume was 10 μL and the detection wavelengths were set to 280, 330 and 350 nm. Data were processed using the Agilent OpenLAB CDS ChemStation edition 2012. Peak assignment of phenolic compounds was carried out comparing their retention times and UV-vis spectra with those of analytically pure standard (Table 7), and as well as by adding the standard solution to the sample.

Analyses of individual phenolic compounds were carried out using an Agilent 1260 series HPLC instrument (Agilent Technologies, Palo Alto, CA, USA) equipped with a quaternary pump, degasser, column thermostat, auto-sampler and diode array detector. Chromatographic separation was carried out according to Molinu et al. [52 (link)]. The column was a Zorbax Eclipse plus C18 (250 × 4.6 mm, 5 µm; Agilent). Column temperature was set to 30 °C and the flow rate was 0.8 mL min⁻¹. The injection volume was 10 μL and the detection wavelengths were set to 280, 330 and 350 nm. Data were processed using the Agilent OpenLAB CDS ChemStation edition 2012. The concentration of six phenolic compounds (neoclorogenic acid, clorogenic acid, criptoclorogenic acid, verbascoside, diosmin, luteolin) were carried out comparing their UV-vis spectra and retention times with those of analytically pure standard.