HeLa cells were obtained from the Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences. Cells were cultured in DMEM medium containing 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml streptomycin. Doxorubicin was purchased from Meilun Biotechnology Co. LTD, Dalian, China.
Doxorubicin
Manufactured by Meilun
Sourced in China
Doxorubicin is a cytotoxic drug used in laboratory research and clinical settings. It is an anthracycline antibiotic that inhibits the growth and proliferation of cells by interfering with DNA and RNA synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Daunorubicin, by Meilun (3 mentions)
Sp600125, by Meilun (2 mentions)
Sorafenib, by Meilun (2 mentions)
Thiazolyl blue tetrazolium mtt, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Beyotime (1 mentions)
Fitc labeled goat anti mouse igg h l, by Beyotime (1 mentions)
Vcam 1 antibody, by Santa Cruz Biotechnology (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
Annexin 5 apc, by BD (1 mentions)
Acetonitrile, by Honeywell (1 mentions)
P gp antibody, by Abcam (1 mentions)
Ecl kit, by Promega (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Calcein am, by Thermo Fisher Scientific (1 mentions)
Nonyl acridine orange, by Merck Group (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Ketoconazole, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide, by Merck Group (1 mentions)
Atp level detection kit, by Beyotime (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
13 protocols using doxorubicin
1
Doxorubicin Cytotoxicity in HeLa Cells
2
Doxorubicin-loaded PLGA Nanoparticles
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Doxorubicin was purchased from Meilunbio (Dalian, China). Succinobucol and VCAM-1 antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Poly (lactic-co-glycolic acid) (PLGA, 50:50, 12,000 Da) was purchased from LACTEL Absorbable Polymers (Birmingham, AL, USA). DSPE-mPEG2000 was purchased from Peng Sheng Biotechnology (Shanghai, China). Acetonitrile was purchased from Honeywell (Charlotte, NC, USA). Phosphate buffer saline (PBS), 4, 6-diamidino-2-phenylindole (DAPI), 4% paraformaldehyde, FITC-labeled goat anti-mouse IgG (H + L), and Hoechst 33342 were purchased from Beyotime Biotechnology (Shanghai, China). Thiazolyl blue tetrazolium (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). APC-Annexin-V was purchased from BD Biosciences Pharmingen (San Diego, CA, USA). All other solvents and reagents were analytical.
3
Doxorubicin-Loaded TPGS Nanoparticles for Multidrug-Resistant Gastric Cancer
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Doxorubicin was obtained from Dalian Meilun Biotech Co., Ltd (Dalian, China). D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) was purchased from Ai Keda Chemical Technology Co., Ltd (Chengdu, China). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) was obtained from Sigma Chemical Corporation (USA). Indocyanine green was obtained from Meilunbio (China). All other chemicals were of reagent grade. Water was purified with a Milli–Q Plus 185 water purification system (Millipore, Bedford, MA). Multidrug-resistant gastric cancer cell line SCG7901 was gift by the Fourth Military Medical University Chinese Academy of Sciences, and the cells were purchased commercially by them. DAPI, Calcein-AM, and propidium iodide (PI) were obtained from Invitrogen (USA). Cell lysates (RIPA) and Protease inhibitor (PMSF) were bought from Sigma Chemical Corporation (USA). P-gp antibody was obtained from Abcam. HRP-labeled goat antimouse IgG secondary antibody was got from Sunshine bio (China), and ECL kit was obtained from Promega (China).
4
Evaluating Multi-Drug Resistance Modulators
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AC, BAC, and aconine (purity >98%) were purchased from Chengdu Mansite Pharmaceutical Co., Ltd. (Chengdu, China). Vincristine, doxorubicin and tariquidar (purity >98%) were purchased from Dalian Meilun Biological Technology Co., Ltd. (Dalian, China). Rifampicin, verapamil, rhodamine 123, dimethyl sulfoxide (DMSO), ketoconazole, phenylmethylsulfonyl fluoride, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and nonyl acridine orange (NAO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). RIPA lysis buffer, BCA protein assay kit, and ATP level detection kit were purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). All other chemicals and solvents were of analytical grade or better and used as received.
5
Doxorubicin and Melatonin Interaction Study
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Doxorubicin was purchased from Meilunbio (A0520AS). Melatonin was purchased from Yuanye biotechnology (B21269). Dimethyl sulfoxide (DMSO) and Trizol Reagent were purchased from Invitrogen. Primary antibodies against YAP were purchased from Cell Signaling Technology (14074S). Antibodies against Bax and Bcl‐2 were purchased from Proteintech (50599‐2‐lg and 12789‐1‐AP). Antibodies against β‐actin, as well as goat anti‐rabbit and goat anti‐mouse secondary antibodies were purchased from Zhongshan Company. Cell Counting Kit‐8 (CCK‐8) was provided by Dojindo. Terminal deoxynucleotidyl transferase dUTP nick‐end labelling (TUNEL) was purchased from Roche Diagnostics. The 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA), and mitochondrial membrane potential assay kit with JC‐1, were purchased from Beyotime Institute of Biotechnology. Histostain™‐SP Kits (SPN‐9002) and DAB (ZLI‐9017) were purchased from Zhongshan Company. FITC‐labelled wheat germ agglutinin(WGA) was purchased from Sigma (L4895).
6
Hyaluronic Acid-Based Nanomedicine Synthesis
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Hyaluronic acid (HA, 97%) (MW ≈ 9000 Da) was purchased from Shandong Freda Biopharmaceutical Co., Ltd. Cysteamine dihydrochloride (>97%), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%), and glutathione (GSH, 98%) were purchased from Aladdin (Shanghai, China). Iron chloride hexahydrate (FeCl3·6H2O, 99%) was purchased from Sigma-Aldrich. Epigallocatechin-3-gallate (EGCG, 95%) and Doxorubicin (DOX, >98%) were purchased from Meilun Biotechnology Co., Ltd. Annexin V-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (7AAD), 4′,6-diamidino-2-phenylindole (DAPI), and lysosomal probes were purchased from Beyotime Biotechnology (Shanghai). Antibodies against CD44, CD31, Ki67, β-actin, and caspase 3 were purchased from Abcam (Massachusetts, USA).
7
Stimuli-Responsive Polymeric Nanocarriers
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Materials L-Lactide (LLA) was obtained from Sinobiom and recrystallized from dry ethyl acetate before use. PEG (M w 4000) and NIPAM were purchased from Aldrich. Stannous 2-ethyl-hexanoate (Sn(Oct) 2 ), methacrylic anhydride and Irgacure 2959 were purchased from Aike, and used as received. Dichloromethane and triethylamine (TEA) were dried over calcium hydride and distilled prior to use. Doxorubicin and tetracycline were purchased from MeilunBio.
8
Drug Synthesis and Characterization
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TMZ hydrochloride tablets were supplied by Servier Pharmaceutical Co., Ltd. (Tianjin, China), while doxorubicin was produced by Dalian Meilun Biotechnology Co., Ltd. (Dalian, China).
9
Doxorubicin-Loaded TPGS Nanoparticles
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Doxorubicin was obtained from Dalian Meilun Biotech Co., Ltd (Dalian, China). D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) was purchased from Ai Keda Chemical Technology Co., Ltd (Chengdu, China). cis-Aconitic anhydride (CA) was received from Meryer Chemical Technology Co., Ltd (Shanghai, China). N,N′-Dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), triethylamine (TEA), anhydrous dimethyl sulfoxide (DMSO), and tetrahydrofuran (THF) were purchased from Aladdin Reagent Co., Ltd (Shanghai, China). 3-[4,5-Dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (MTT) was obtained from Sigma Chemical Corporation (USA). Annexin V-FITC/PI Apoptosis Detection Kit was purchased from Yeasen Corporation (Shanghai, China). Hoechst 33342 and 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were purchased from Invitrogen Corporation (Carlsbad, CA, USA). Chlorin e6 (Ce6) was obtained from Frontier Scientific (Utah, USA). All other chemicals were of reagent grade. Water was purified with a Milli-Q Plus 185 water purification system (Millipore, Bedford, MA).
10
Combination Cancer Treatment Protocol
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Metformin, 2-deoxyglucose, doxorubicin, vera-pamil, digoxin, MTT were all purchased from Dalian Meilun Biotech Co., Ltd.(China). p53 activator AB143228 was purchased from Abcam Company. p53 inhibitor PFTα was obtained from Selleck Chemicals LLC.
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