Sodium acetate

Manufactured by Sangon

Sourced in Germany, China

Sodium acetate is a common laboratory chemical used as a buffer solution. It is a white crystalline solid that is soluble in water and has a variety of applications in analytical and experimental procedures.

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Lab products found in correlation

Ethanol, by Sangon (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Sangon (2 mentions) Sephadex g 25, by GE Healthcare (2 mentions) Amicon ultra centrifugal filter, by Merck Group (2 mentions) Sodium chloride (nacl), by Sangon (2 mentions) Imidazole, by Sangon (2 mentions) Magnesium chloride, by Sangon (2 mentions) Ni nta agarose, by GE Healthcare (2 mentions) Acetic acid, by Sangon (2 mentions) Fetal bovine serum (fbs), by Wisent (1 mentions) 5 fluorodeoxyuridine, by Thermo Fisher Scientific (1 mentions) N n diisopropylethylamine dipea, by Maclin Biochemical Technology (1 mentions) 2 cyanoethyl n n n n tetraisopropylphosphordiamidite, by Maclin Biochemical Technology (1 mentions) Rpmi 1640 medium, by Wisent (1 mentions) Dmem medium, by Wisent (1 mentions) Trypsin, by Wisent (1 mentions) Tris base, by Sangon (1 mentions) Ethylenediaminetetraacetic acid edta, by Sangon (1 mentions) Antibiotic antimycotic 100, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

3 protocols using sodium acetate

Reagents for Biomolecular Modification

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5-Fluorodeoxyuridine was purchased from Alfa Aesar (Shanghai, China). 4,4ʹ-Dimethoxytrityl Chloride (DMT-Cl, 97%) N,N-Diisopropylethylamine (DIPEA, 97%) and 2-Cyanoethyl N,N,N’,N’-tetraisopropylphosphordiamidite (95%) were obtained from Macklin Inc. (Shanghai, China). N-(ε-malemidocaproyloxy) succinimide ester (EMCS) was purchased from Aladdin (Shanghai, China). Ni-NTA agarose, and Sephadex G-25 were obtained from GE Healthcare (Piscataway, USA). Imidazole, sodium chloride, sodium acetate, polyacrylamide, tris base, acetic acid, ethylenediaminetetraacetic acid (EDTA), magnesium chloride, ethanol, and DAPI were obtained from Sangon Biotech (Shanghai) Co., Ltd (China). Amicon ultracentrifugal filters were purchased from Merck Millipore Ltd (Darmstadt, Germany). RPMI 1640 medium, DMEM medium, MEBM medium, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), antibiotic-antimycotic (100×) and fetal bovine serum (FBS) were purchased from Wisent Biotechnology (Nanjing) Co. Ltd (China). All chemicals were used without further purification.

Zhang C., Han M., Zhang F., Yang X., Du J., Zhang H., Li W, & Chen S. (2020). Enhancing Antitumor Efficacy of Nucleoside Analog 5-Fluorodeoxyuridine on HER2-Overexpressing Breast Cancer by Affibody-Engineered DNA Nanoparticle. International Journal of Nanomedicine, 15, 885-900.

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Synthesis and Characterization of EMCS-Pt Conjugate

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The DNAs were synthesized from Sangon Biotech (Shanghai) Co., Ltd. N-(ε-maleimidocaproyloxy)succinimide ester (EMCS) and cis-platinum was purchased from Aladdin (Shanghai, China). Ni-NTA agarose and Sephadex G-25 were obtained from GE Healthcare (Piscataway, NJ, USA). Imidazole, sodium chloride, sodium acetate, polyacrylamide, trizma base, acetic acid, ethylenediaminetetraacetic acid (EDTA), magnesium chloride, ethanol, sodium diethyldithiocarbamate (DDTC) and DAPI were obtained from Sangon Biotech (Shanghai) Co., Ltd. Amicon® ultracentrifugal filters were purchased from Merck Millipore Ltd. (Darmstadt, Germany). Gibco® RPMI 1640 medium, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), dimethylsulfoxide (DMSO), antibiotic–antimycotic (100×), fetal bovine serum (FBS), and DAPI were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All chemicals were purchased and used without further purification.

Zhang C., Zhang H., Han M., Yang X., Pei C., Xu Z., Du J., Li W, & Chen S. (2019). DNA–affibody nanoparticle delivery system for cisplatin-based breast cancer chemotherapy. RSC Advances, 9(4), 1982-1989.

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Chemotaxis Paralysis Assay in Nematodes

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Chemotaxis paralysis assay was performed as described previously [22 ], 1 M Sodium acetate (Sangon Biotech, China) and 1 M Sodium azide (Sangon Biotech, China) (1:1) were blended as attractants. 1 M sterile water and 1 M Sodium acetate (1:1) were blended as the control odorant. Synchronized L1 CL2355 nematodes were maintained on a 100 mm NGM culture plate with E. coli OP50, liensinine and neferine. Cultured at 16 °C for 42 h and then raised temperature to 25 °C for 32 h. Washed the nematodes with M9 buffer to remove the E. coli OP50 and drugs, and transferred the nematodes to the center of an uncoated 100 mm NGM plate. Dripped 10 µL attractant and 10 µL control odorant on both sides of the plate respectively, placed upside down at room temperature for one hour. Calculated the chemotaxis index (CI) = (number of nematodes on the attractant side − number of nematodes on the control side)/total number of nematodes.

Wu M.C., Gao Y.H., Zhang C., Ma B.T., Lin H.R., Jiang J.Y., Xue M.F., Li S, & Wang H.B. (2023). Liensinine and neferine exert neuroprotective effects via the autophagy pathway in transgenic Caenorhabditis elegans. BMC Complementary Medicine and Therapies, 23, 386.

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