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Trpc6 acc 017

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TRPC6 (ACC-017) is a recombinant protein that represents the full-length human Transient Receptor Potential Cation Channel Subfamily C Member 6 (TRPC6) protein. TRPC6 is a non-selective cation channel that is involved in calcium signaling and homeostasis.

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Lab products found in correlation

Tubulin, by Thermo Fisher Scientific (1 mentions) Laemmli 6x sds sample buffer, by Boston BioProducts (1 mentions) Esrp1, by Thermo Fisher Scientific (1 mentions) Chloroquine c6628, by Merck Group (1 mentions) U0126 hy 12031, by MedChemExpress (1 mentions) Erk1 2 4695, by Cell Signaling Technology (1 mentions) Talin, by Cell Signaling Technology (1 mentions) Actin, by Cell Signaling Technology (1 mentions) U46619, by Cayman Chemical (1 mentions) Gsk1702934a gsk, by Focus Biomolecules (1 mentions) Phospho mlc2, by Cell Signaling Technology (1 mentions) Trpc6 pam c20, by Bio-Techne (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions)

3 protocols using trpc6 acc 017

1

Detailed Immunoblotting and qPCR Protocol

Cited 1 time
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For immunoblotting, adherent cells were washed with PBS and scraped on ice in radioimmunoprecipitation assay (RIPA) buffer, which was supplemented with protease and phosphatase inhibitors (Roche). Laemmli 6X SDS sample buffer (BP-111R, Boston BioProducts) was added to each sample, which were boiled for 10 min prior to SDS-polyacrylamide gel electrophoresis. Immunoblotting primary antibodies were used in the following dilutions: TRPC6 ACC-017 (Alomone Labs) 1:1000, Tubulin and GAPDH 1:2000, ESRP1 (Thermofisher) 1:2000, α6A 1:1000, α6B 1:1000, HA 1:2000. RhoA activity was assessed using a GST fusion protein containing the Rho-binding domain of ROCK (RBD) as previously described59 (link),62 (link)mRNA quantification was accomplished using an RNA isolation kit (BS88133, Bio Basic Inc.), and complementary DNAs (cDNAs) were produced using an Azura cDNA synthesis kit (AZ-1996, Azura Genomics). Azura View GreenFast qPCR Blue Mix LR was used as the qPCR Master Mix (AZ-1996, Azura Genomics).
Mukhopadhyay D., Goel H.L., Xiong C., Goel S., Kumar A., Li R., Zhu L.J., Clark J.L., Brehm M.A, & Mercurio A.M. (2023). The calcium channel TRPC6 promotes chemotherapy-induced persistence by regulating integrin α6 mRNA splicing. Cell reports, 42(11), 113347.
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2

Signaling Pathways Regulation Protocol

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The primary antibodies against Akt (9272), p-Akt (Ser473) (4060 P), cleaved caspase-3 (9661), p-p70S6K (9205), p70S6K (9202), p-ERK1/2 (4370), and ERK1/2 (4695) were purchased from Cell Signaling Technology. The primary antibodies against TRPC3 (ACC-016) and TRPC6 (ACC-017) were purchased from Alomone. The primary antibody against LC3B (L7543) was purchased from Sigma-Aldrich. The primary antibody against β-actin (TA-09) was purchased from the ZhongShan Biotechnology Company. The secondary antibodies HRP-conjugated anti-rabbit IgG (110777) and anti-mouse IgG (117228) were purchased from the KeRui Biotechnology Company. H2O2 (107298) was purchased from Millipore. Chloroquine (C6628) and t-BOOH (458139) were purchased from Sigma-Aldrich. SAR7334 (HY-15699), bafilomycin A1 (HY-100558), MK2206 (HY-10358), and U0126 (HY-12031) were purchased from MedChemExpress.
Hou X., Xiao H., Zhang Y., Zeng X., Huang M., Chen X., Birnbaumer L, & Liao Y. (2018). Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. Cell Death & Disease, 9(10), 1015.
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3

TRPC6 Activation and Signaling Assay

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Chemicals were purchased from Sigma Aldrich unless otherwise specified. GSK1702934A (GSK) was from Focus Biomolecules, TRPC6-PAM-C20 from Tocris, Ani 9 and U46619 from Cayman Chemical. Antibodies were from the following sources: Actin (#3700), Erk1/2 (#4695), phospho-Erk1/2 (#4370), phospho-MLC2 (#3675), Talin (#4021), all from Cell Signaling Technology; TRPC6 (ACC-017) from Alomone; FITC Annexin V (556419) and PE CD41 (561850) from BD Biosciences.
Boekell K.L., Brown B.J., Talbot B.E, & Schlondorff J.S. (2022). Trpc6 gain-of-function disease mutation enhances phosphatidylserine exposure in murine platelets. PLoS ONE, 17(6), e0270431.
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