5 kd mwco filter

Manufactured by Merck Group

The 5 kD MWCO (Molecular Weight Cut-Off) filter is a laboratory equipment designed to separate and concentrate molecules or particles based on their molecular weight. It functions by allowing the passage of smaller molecules or particles through its porous membrane, while retaining larger molecules or particles. This filter is commonly used in various applications, such as sample preparation, protein purification, and buffer exchange.

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Lab products found in correlation

Phosphodiesterase 1, by Merck Group (2 mentions) E coli alkaline phosphatase, by Merck Group (2 mentions) Nuclease p1, by Merck Group (2 mentions)

Spelling variants of this product

MWCO) filters (28 citations)

2 protocols using 5 kd mwco filter

Bulk tRNA Preparation and Nucleoside Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bulk tRNAs were prepared as previously described using acid buffered-phenol
(phenol saturated with 50 mM sodium acetate, pH 5.8) and alcohol precipitation
20 (link). Nucleosides were prepared as
described in 61 (link) by hydrolyzing bulk tRNA
with 10 units of Nuclease P1 (Sigma) overnight at 37°C, with the addition of
0.01 units of phosphodiesterase I (Sigma) and 3 µL E. colialkaline phosphatase (Sigma). The hydrolyzed nucleosides were further purified
by filtering through a 5 kD MWCO filter (Millipore) (to remove enzymes), dried
in a CentriVap Concentrator, and suspended in 20 µL of water prior to analysis
by HPLC or LC-MS/MS.

Thiaville P.C., Legendre R., Rojas-Benítez D., Baudin-Baillieu A., Hatin I., Chalancon G., Glavic A., Namy O, & de Crécy-Lagard V. (2015). Global translational impacts of the loss of the tRNA modification t6A in yeast. Microbial Cell, 3(1), 29-45.

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Bulk tRNA Isolation and Nucleoside Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bulk tRNAs were prepared as previously described using acid buffered-phenol (phenol saturated with 50 mM sodium acetate, pH 5.8) and alcohol precipitation [20 (link)]. Nucleosides were prepared as described in [61 (link)] by hydrolyzing bulk tRNA with 10 units of Nuclease P1 (Sigma) overnight at 37°C, with the addition of 0.01 units of phosphodiesterase I (Sigma) and 3 µL E. coli alkaline phosphatase (Sigma). The hydrolyzed nucleosides were further purified by filtering through a 5 kD MWCO filter (Millipore) (to remove enzymes), dried in a CentriVap Concentrator, and suspended in 20 µL of water prior to analysis by HPLC or LC-MS/MS.

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