Surface stains were performed followed by staining of dead cells with Fixable Violet Dead Cell Stain kit (L34964; ThermoFisher). A Foxp3 transcription factor staining buffer kit (eBioscience) was used to fix and permeabilize cells. Finally, these antibodies were used for transcription factor staining PE-conjugated to anti-mouse/human GATA-3 (TWAJ; eBioisciences), anti mouse/human cMAF (SymOF1; eBiosciences), anti mouse/human IRF4 (3E4; eBiosciences), anti mouse/human BATF (9B5A13); and AlexaFluor 647 conjugated to anti-mouse BCL6 (K112-91; BD Biosciences).
Anti mouse bcl6 k112 91
Manufactured by BD
Anti-mouse BCL6 (K112-91) is a laboratory reagent used for detection of the BCL6 protein in mouse samples. It is a monoclonal antibody that specifically binds to the BCL6 protein expressed in mouse cells.
Automatically generated - may contain errors
Lab products found in correlation
Foxp3 transcription factor staining buffer kit, by Thermo Fisher Scientific (2 mentions)
Fixable violet dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Symof1, by Thermo Fisher Scientific (2 mentions)
Anti mouse human irf4 3e4, by Thermo Fisher Scientific (2 mentions)
Lsrfortessa x 20 analyzer, by BD (1 mentions)
Cd38 90, by Thermo Fisher Scientific (1 mentions)
Anti b220, by BD (1 mentions)
Streptavidin bv785, by BioLegend (1 mentions)
Cd138 281 2, by BioLegend (1 mentions)
Cd95 jo2, by BD (1 mentions)
Cd45.1 a20, by BioLegend (1 mentions)
Cd45.2 104, by BioLegend (1 mentions)
Ra3 6b2, by BioLegend (1 mentions)
7 aad, by BD (1 mentions)
Annexin 5, by BD (1 mentions)
3 protocols using anti mouse bcl6 k112 91
1
Transcription Factor Profiling by Flow Cytometry
2
Flow Cytometry Analysis of B Cell Differentiation
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The flow cytometry analysis was performed using the BD LSRFortessa™ X-20 analyzer (BD Biosciences). Mouse splenic cells were stained with anti-B220 (RA3-6B2, BioLegend), CD38 (90, eBioscience), CD95 (Jo2, BD), CD45.1 (A20, BioLegend), and CD45.2 (104, BioLegend) for the in vivo study of B1-8hi cell differentiation or with anti-B220, CD95, GL7 (GL7, BioLegend), CD138 (281-2, BioLegend), biotin anti-mouse IgG1 (RMG1-1, BioLegend), and BV785 streptavidin (BioLegend) for the in vitro analysis of GC B cell and plasma cell differentiation in the co-culture system or with anti-B220, annexin V (BD), and 7AAD (BD) for the analysis of cell apoptosis. The transduced A20 cell lines (kindly provided by Dr. Jeffrey Ravetch, Rockefeller University) were stained with anti-mouse BCL6 (K112-91, BD) for the knockdown efficiency of Bcl6-specific shRNA.
3
Transcription Factor Profiling by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Surface stains were performed followed by staining of dead cells with Fixable Violet Dead Cell Stain kit (L34964; ThermoFisher). A Foxp3 transcription factor staining buffer kit (eBioscience) was used to fix and permeabilize cells. Finally, these antibodies were used for transcription factor staining PE-conjugated to anti-mouse/human GATA-3 (TWAJ; eBioisciences), anti mouse/human cMAF (SymOF1; eBiosciences), anti mouse/human IRF4 (3E4; eBiosciences), anti mouse/human BATF (9B5A13); and AlexaFluor 647 conjugated to anti-mouse BCL6 (K112-91; BD Biosciences).
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