Methanol (CH3OH), ammonium acetate (CH3COONH4), acetonitrile (CH3CN), picolinic acid, 3-hydroxykynurenine, quinolinic acid, serotonin, 5-hydroxytryptophan, kynurenine, 3-hydroxyanthranilic acid, tryptamine, L-tryptophan, 5-hydroxyindole, acetic acid, indoxyl sulfate, N-Acetylserotonin, xanthurenic acid, indole-3-acetamide, kynurenic acid, DL-indole-3-lactic acid, indole-3-carboxaldehyde, indole-3-acetic acid, tryptophol, melatonin, 5-hydroxyindole acetic acid-D5, serotonin-D4, indole-3-lactic acid-D4, kynurenic acid-D5, melatonin-D4, picolinic acid-D3, tryptamine-D4, xanthurenic acid-D4, 3-hydroxyanthranilic acid-D3, 3-hydroxykynurenine-13C2-15N, 5-hydroxytryptophan-D4, indole-3-acetamide-D5, kynurenine-D4, L-tryptophan-13C11,15N, and tryptophan-D5 were purchased from Sigma Aldrich, Merck KGaA (Darmstadt, Germany) and other reagents and chemicals used in the study were purchased from local suppliers. The reagents and chemicals were of analytical grade or higher purity. Additionally, the yeast strain S. cerevisiae STG S101 was purchased from Fermentis (Marcq-En-Baroeul, France).
Kynurenine d4
Manufactured by Merck Group
Sourced in Germany
Kynurenine-D4 is a deuterium-labeled amino acid used as an internal standard in the quantitative analysis of kynurenine levels in biological samples. It is a stable-isotope labeled compound that can be detected and quantified alongside the unlabeled kynurenine analyte using mass spectrometry techniques.
Automatically generated - may contain errors
Lab products found in correlation
Picolinic acid, by Merck Group (1 mentions)
Xanthurenic acid, by Merck Group (1 mentions)
Tryptamine, by Merck Group (1 mentions)
Serotonin, by Merck Group (1 mentions)
Indole 3 lactic acid, by Merck Group (1 mentions)
Melatonin, by Merck Group (1 mentions)
L tryptophan, by Merck Group (1 mentions)
3 hydroxyanthranilic acid, by Merck Group (1 mentions)
3 hydroxykynurenine, by Merck Group (1 mentions)
D5 tryptophan, by Merck Group (1 mentions)
Indole 3 acetamide, by Merck Group (1 mentions)
Dl indole 3 lactic acid, by Merck Group (1 mentions)
5 hydroxytryptophan, by Merck Group (1 mentions)
Kynurenic acid, by Merck Group (1 mentions)
Quinolinic acid, by Merck Group (1 mentions)
2 protocols using kynurenine d4
1
Tryptophan Metabolism Metabolite Analysis
2
Quantification of Plasma Kynurenine and Tryptophan
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Plasma kynurenine and tryptophan were measured with an Acquity TQ-S UPLC-tandem mass spectrometer (Waters, USA), using reference compounds (L-kynurenine and L-tryptophan) and deuterated tryptophan-d5 and kynurenine-d4 as internal standards (Sigma-Aldrich, South Africa). The plasma samples stored at -70 °C were thawed at room temperature, followed by spiking with internal standards, deproteination with absolute methanol, and vortexing for 10 min. The samples were then centrifuged at 3156 rcf (relative centrifugal force) for 10 min, and 10 μL supernatant was injected in the UPLC-MS/MS system. The analytes were separated using an isocratic elution of injected samples within 2 min. Kynurenine and tryptophan were detected in the multiple reaction mode using electrospray ionization MS (ESI + ) in the positive mode. This method has been previously published ( Adu-Gyamfi et al.,
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