Breast cancer cell lines MCF-7, MDA-MB-231, MDA-MB-453, T47-D, SKBR-3 and MDA-MB-468 were procured from National Centre for Cell Sciences, India. MCF-10A cells (immortalised normal breast epithelial cell line) were obtained from American Type Culture Collection (ATCC) and maintained as per the instructions given in ATCC. Cancer cells were maintained routinely in Dulbecco’s Modified Eagle’s medium with 10% foetal bovine serum and 1% penicillin–streptomycin solution in an incubator (95% relative humidity; 5% CO2; 37 °C). Experiments were performed with cells of fewer than eight passages after thawing the frozen stock. Testing for mycoplasma contamination was carried out at regular intervals. Cell line authentication was performed for the cell lines used in the study by short tandem repeat profiling.
Mda mb 453
Manufactured by National Centre for Cell Science
Sourced in India
MDA-MB-453 is a well-characterized human breast cancer cell line that is commonly used in research. It is a triple-negative breast cancer cell line, meaning it lacks expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2. The MDA-MB-453 cell line is a valuable tool for studying various aspects of breast cancer biology and for evaluating potential therapeutic interventions.
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Lab products found in correlation
Mda mb 231, by National Centre for Cell Science (6 mentions)
Mcf 7, by National Centre for Cell Science (6 mentions)
Mda mb 468, by National Centre for Cell Science (4 mentions)
Mcf10a cells, by American Type Culture Collection (2 mentions)
Sk br 3, by National Centre for Cell Science (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Sigma lookout mycoplasma pcr detection kit, by Merck Group (1 mentions)
Co2 incubator, by Eppendorf (1 mentions)
Mda mb 435s, by National Centre for Cell Science (1 mentions)
Hbl 100, by National Centre for Cell Science (1 mentions)
7 protocols using mda mb 453
1
Breast Cancer Cell Line Maintenance
2
Culturing Breast Cancer Cell Lines
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We obtained breast cancer cell lines (MDA-MB-468, MDA-MB-453, MDA-MB-231, T47D and MCF-7) from National Centre for Cell Science (NCCS), Pune, India. The cells were cultured in the high glucose DMEM medium. The media was supplemented with FBS (10%), streptomycin (100 µg/mL) and penicillin (100 units/mL).
3
Cell Line Characterization and Maintenance Protocol
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MDA-MB-231 (BRAF, c.1391G>T, p.G464V; KRAS, c.38G>A p.G13D), MCF-7 (PIK3CA, c.1633G>A, p.E545K), MDA-MB-361 (PIK3CA, c.1633G>A p.E545K) and MDA-MB-453 (BRAF, KRAS and PIK3CA wild type) were purchased from the National Centre for Cell Science (NCCS, Pune, India). The cell lines were routinely cultured in 75-mL tissue culture flasks (Nunc Thermofisher Scientific) in RPMI 1640 medium supplemented with 10% heat inactivated fetal bovine serum, 2 mM L-glutamine, 100 units/ml penicillin, 100 μg/ml streptomycin (all from Sigma, USA) at 37°C and 5% CO2, according to the protocol provided by NCCS. All cell lines were tested for Mycoplasma detection by the Sigma LookOut Mycoplasma PCR Detection Kit (MP0035).
4
Breast Cancer Cell Line Culture
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The human breast cancer cell lines MCF-7, MDA-MB-231, and MDA-MB-453 were purchased from the National Centre for Cell Science (NCCS), Pune, India. The cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin as antibiotics in a T-25 flask at 37 °C in a humidified atmosphere of 5% CO2 in a CO2 incubator (Eppendorf, Germany). All experiments were performed using cells from passage 15 or less.
5
Culturing Breast Cancer Cell Lines
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Human breast cancer cell lines MDA-MB-231 and MDA-MB-453 were purchased from the National Centre for Cell Sciences, Pune, India. MCF-10A cells were procured from ATCC, USA. The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) media and supplemented with 10% heat-inactivated fetal bovine serum (FBS), 0.1 mg/mL of streptomycin, and 100 units/mL of penicillin at 37 °C in a humidified atmosphere with 5% CO2 [12 (link)].
6
Culturing Breast Cancer Cell Lines
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Human Breast Cancer cell lines- MCF-7, MDA-MB-453, MDA-MB-435S, MDA-MB-231, MDA-MB-468 were procured from National Centre for Cell Science, Pune, India. The cells were grown in DMEM, L15 medium, while 10 % FBS, sodium pyruvate, penicillin-streptomycin, l -glutamine, solution was given as supplements and the cells were cultured in T-25 flasks incubated at 37 °C incubator with 5% CO2.
7
Comprehensive Breast Cancer Cell Line Characterization
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The human breast cancer cell lines T47D, MDA MB 453, MDA MB 468, HBL 100, MCF7 and ZR75 were purchased from National Centre for Cell Science, India. MCF10A and MCF10AT were purchased from Karmanos Cancer Centre, USA. MCF10DCIS was purchased from Asterand Bioscience, USA. Rat-1 fibroblast cell line was a gift from Dr Robert A. Weinberg (Whitehead Institute for Biomedical Research, USA). MDA MB 231, SKBR3 and Hs587t were gift from Dr Asha Nair from Rajiv Gandhi Centre for Biotechnology, India. BT474 was a gift from Cancer Institute, Adayar, India. Matched breast tumour and adjacent normal tissues were obtained from Sri Ramachandra Medical College, India after getting ethical clearance. The disease status was confirmed by the pathologist.
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