β4 integrin

The following antibodies were used for immunostaining: TCF3 (1:100; Fuchs laboratory^{18 (link)}), TCF4 (1:200; Cell Signaling Technology, 2569), LEF1 (1:1,000; Cell Signaling Technology, 2230), TCF1 (1:200; Cell Signaling Technology, 2203), CD34 (1:100; eBiosciences, 13-0341), SOX9 (1:1,000; Fuchs laboratory^{56 (link)}), β-catenin (1:500; BD, clone 14), GFP (1:5,000; Abcam, ab13970), Keratin 5 (1:200; Covance, PRB-160P), Keratin 10 (1:200; Covance, PRB-159P), Integrin β4 (1:100; BD, 553745), PPARγ (1:100; Santa Cruz Biotechnology, H-100, sc-7196). The following antibodies were used for immunoprecipitation and immunoblotting: TCF3 (for immunoprecipitation, Fuchs laboratory^{18 (link)}; for immunoblotting, 1:1,000; Santa Cruz Biotechnology, V-17, sc-12491), TCF4 (1:1,000; Santa Cruz Biotechnology, N-20, sc-8631), TLE (1:1,000; Santa Cruz Biotechnology, C-19, sc-13373), HDAC1 (1:1,000; Abcam, ab7028), β-catenin (1:2,000; Sigma, C7207), α-tubulin (1:2,000; Millipore, 05829). The following antibodies were used for FACS: integrin α6 (1:500; eBiosciences, PE-conjugated, 12-0495; PE-Cy7-conjugated, 25-0495), CD34 (1:100; eBiosciences, Alexa Fluor 660-conjugated, 50-0341), Sca1 (1:1,000; eBiosciences, PerCP-Cy5.5-conjugated, 45-5981).

Antibodies against Vimentin, and αSMA were purchased from Sigma-Aldrich. N-cadherin, p44/42 MAPK, Phospho-p44/42 MAPK, EGFR antibodies were from Cell Signaling Technology (Danvers, MA); α-tubulin and ROBO1 from Abcam (Cambridge, MA). Fibronectin, Integrin αV, Integrin α2, Integrin α5, Integrin β1, Integrin β4 (BD Biosciences); MMP14 (Epitomics, Burlingam, CA); Ki67 Vector Laboratories (Burlingame, CA).