Gpnmb

For immunohistochemistry experiments, antibodies against Tfeb/TFEB (Cat. No. A303-673A, Bethyl Laboratories), cathepsin B (CTSB; Cat. No. 31718, Cell Signaling Technologies), cathepsin D (CTSD; Cat. No. 2284, Cell Signaling Technologies), transcription factor E3 (TFE3; Cat. No. PA5-54909, Invitrogen), phospho-rpS6:Ser^235/236 (Cat. No. 4858, Cell Signaling Technologies), and glycoprotein nonmetastatic melanoma protein B (GPNMB; Cat. No. PA5-42585, Thermo Fisher Scientific) were used. For immunocytochemistry experiments, TFEB (Cat. No. PA1-31552, Invitrogen), late endosomal/lysosomal adaptor and MAPK and mTOR activator 4 (LAMTOR4; Cat. No. 13140, Cell Signaling Technologies), light chain 3B (LC3B; Cat. No. ab192890, Abcam), and sequestosome 1 (SQSTM1)/p62 (Cat. No. 23214, Cell Signaling Technologies) were used. For three-dimensional (3-D) cyst culture experiments, TFEB (Cat. No. A303-673A, Bethyl Laboratories) was used.
Fluorescent-conjugated secondary antibodies were purchased from Abcam (Gt α rabbit DyLight 488, Cat. No. ab96899; Gt α mouse DyLight 488, Cat. No. ab96879; Gt α mouse DyLight 550, Cat. No. ab96872; and Gt α rabbit DyLight 550, Cat. No. ab96884).

Cells grown in 6-well plates were lysed in RIPA buffer containing protease inhibitors. The blots were probed with antibodies against collagen I (COL1, Abcam), α-smooth muscle actin (αSMA, Abcam), ADAM10 (Novus), CD44, and GPNMB (both from Cell Signaling Technology). For loading control, the blots were immunoblotted with antibodies against β-actin (Sigma) or GAPDH (Cell Signaling Technology). Band quantification was performed using ImageJ (14 (link)).