Log In Sign up
The largest database of trusted experimental protocols

293freestyle

Manufactured by Thermo Fisher Scientific
Visit Supplier

The 293FreeStyle is a cell line developed for the transient expression of recombinant proteins in suspension culture. It is designed for high-yield protein production in serum-free media.

Automatically generated - may contain errors

Lab products found in correlation

Vp sfm, by Thermo Fisher Scientific (2 mentions) Expi293f, by Thermo Fisher Scientific (1 mentions)

4 protocols using 293freestyle

1

Culturing Human Embryonic Kidney Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies), cultured at 37°C with 8% CO2 and 130 rpm. HEK293T/17 is a female human embryonic kidney cell line (ATCC). VeroE6 is a female kidney epithelial cell from African green monkey. BHK is a kidney cell line from baby hamsters. All adherent cells were cultured at 37°C with 8% CO2 in flasks with DMEM + 10% FBS + 1% penicillin-streptomycin. Cells lines were not tested for mycoplasma contamination nor authenticated.
Walls A.C., Park Y.J., Tortorici M.A., Wall A., McGuire A.T, & Veesler D. (2020). Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell, 181(2), 281-292.e6.
+ Open protocol
+ Expand
2

Cell Line Cultivation Protocols for HEK293 and VeroE6

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies), cultured at 37°C with 5% CO2 and 150 rpm. HEK293T/17 is a female human embryonic kidney cell line (ATCC). HEK293T/17 cells were cultured at 37°C with 5% CO2 in flasks with DMEM + 10% FBS + penicillin-streptomycin + 10mM HEPES. Expi293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). Expi293F cells were grown in Expi293F expression medium, cultured at 37°C, 5% CO2 and 150 rpm. VeroE6 is a female kidney epithelial cell from African green monkey. VeroE6 cells were grown in serum-free medium (VP-SFM; ThermoFisher) at 37°C and 5% CO2. Cells lines were not tested for mycoplasma contamination nor authenticated except for the VeroE6 cells.
Walls A.C., Xiong X., Park Y.J., Tortorici M.A., Snijder J., Quispe J., Cameroni E., Gopal R., Dai M., Lanzavecchia A., Zambon M., Rey F.A., Corti D, & Veesler D. (2019). Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion. Cell, 176(5), 1026-1039.e15.
+ Open protocol
+ Expand
3

Characterization of Human Embryonic Kidney Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies), cultured at 37°C with 5% CO2 and 150 rpm. HEK293T/17 is a female human embryonic kidney cell line (ATCC). HEK293T/17 cells were cultured at 37°C with 5% CO2 in flasks with DMEM + 10% FBS + penicillin-streptomycin + 10mM HEPES. Expi293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). Expi293F cells were grown in Expi293F expression medium, cultured at 37°C, 5% CO2 and 150 rpm. VeroE6 is a female kidney epithelial cell from African green monkey. VeroE6 cells were grown in serum-free medium (VP-SFM; ThermoFisher) at 37°C and 5% CO2. Cells lines were not tested for mycoplasma contamination nor authenticated except for the VeroE6 cells.
Walls A.C., Xiong X., Park Y.J., Tortorici M.A., Snijder J., Quispe J., Cameroni E., Gopal R., Dai M., Lanzavecchia A., Zambon M., Rey F.A., Corti D, & Veesler D. (2019). Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion. Cell, 176(5), 1026-1039.e15.
+ Open protocol
+ Expand
4

Culturing Human Embryonic Kidney Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies), cultured at 37°C with 8% CO2 and 130 rpm. HEK293T/17 is a female human embryonic kidney cell line (ATCC). VeroE6 is a female kidney epithelial cell from African green monkey. BHK is a kidney cell line from baby hamsters. All adherent cells were cultured at 37°C with 8% CO2 in flasks with DMEM + 10% FBS + 1% penicillin-streptomycin. Cells lines were not tested for mycoplasma contamination nor authenticated.
Walls A.C., Park Y.J., Tortorici M.A., Wall A., McGuire A.T, & Veesler D. (2020). Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell, 181(2), 281-292.e6.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!