Barcoded cells were stained with Fc Receptor Blocking Solution (BioLegend, Cat#422302) at 20 mg/mL for 5 min at RT on a shaker. Surface antibody cocktail is then added with a 500 ul final reaction volume for 30 min at RT on a shaker. Following staining, cells were washed twice with cell staining media. Before intracellular staining, cells were permeabilized for 10 min with methanol at 4°C. Methanol is then removed by washing the cells 2 times with cells staining media. Intracellular cocktail is then added to the cells in 500 uL final reaction volume for 1 h at RT on a shaker. Cells were washed twice in cell staining media to remove antibodies excess and then stained with 1mL of 1:4000 191/193Ir Iridium intercalator solution (Fluidigm,Cat#201192B) diluted in PBS with 4% PFA overnight. Before mass cytometry run, cells were washed once with cell staining media, and twice with Cell Acquisition Solution (Fluidigm, Cat# 201240).
Ir iridium intercalator solution
Manufactured by Standard BioTools
191/193Ir Iridium intercalator solution is a laboratory reagent used for various applications in molecular biology and analytical chemistry. It functions as an intercalating agent that binds to DNA or RNA molecules, allowing for their detection and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ir iridium intercalator solution
1
Mass Cytometry Cell Surface and Intracellular Staining
2
Multi-Omics Cell Surface Profiling
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Barcoded cells were stained with Fc Receptor Blocking Solution (BioLegend, Cat#422302) at 20 mg/ml for 5 min at RT on a shaker. Surface antibody cocktail is then added with a 500 ul final reaction volume for 30 min at RT on a shaker. Following staining, cells were washed twice with cell staining media. Before intracellular staining, cells were permeabilized for 10 min with methanol at 4°C. Methanol is then removed by washing the cells 2 times with cells staining media. Intracellular cocktail is then added to the cells in 500 uL final reaction volume for 1 hour at RT on a shaker. Cells were washed twice in cell staining media to remove antibodies excess and then stained with 1mL of 1:4000 191/193Ir Iridium intercalator solution (Fluidigm,Cat#201192B) diluted in PBS with 4% PFA overnight. Before mass cytometry run, cells were washed once with cell staining media, and twice with Cell Acquisition Solution (Fluidigm, Cat# 201240).
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