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2 protocols using sarpogrelate

1

Vasomotor Regulation Protocol

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9,11-dideoxy-11a,9a-epoxy-methanoprostaglandin F2a (U46619), 5-HT, nifedipine, acetylcholine, Fluo-4, sarpogrelate, SB204741, DOI, 2-APB, thapsigargin and U73122 were purchased from Sigma–Aldrich (St. Louis, MO, USA). U-46619, thapsigargin, Fluo-4, SB204741 and nifedipine were dissolved in DMSO and the others in distilled water. Further dilution was made from a stock solution.
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2

Evaluation of Cardioprotective Agents

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Sarpogrelate and cabergoline were obtained from Sigma Aldrich Chemie GmbH, St. Louis, Missouri, USA. Bromocriptine was sourced from Novartis, Milano, Italy. Glibenclamide was obtained from Spimaco (Jeddah, Kingdom of Saudi Arabia). Alloxan, fetal bovine serum, and Dulbecco's modified Eagle medium were obtained from Sigma-Aldrich (St Louis, Missouri). Trypsin and Hanks’ balanced salt solution (HBSS) were collected from HyClone Laboratories (Thermo Fisher Scientific, Waltham, Massachusetts). Insulin ELISA kit (ab100578, lot: GR3279516-1), cardiac troponin 1 ELISA kit (ab246529, lot: GR3309533-1), rat CKM ELISA kit (ab187396, lot: GR327198-1), and ATPase assay colorimetric kit (ab234055, lot: GR3298881-1) were obtained from Abcam (Cambridge, UK). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Gold Biotechnology (St Louis, Missouri).
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