The HDL (10 mg/ml) was dialyzed against borate buffer (pH 8.3) using 20 kDa cut-off dialysis cassette for 1 hr. The HDL was labelled by incubation with 25 μg of Alexa 594 NHS (10 mg/ml in anhydrous dimethylformamide) overnight at room temperature with constant mixing. The free Alexa-594 dye was removed by dialysis using 20 kDa cut-off dialysis cassette against 50 mM HEPES buffer for 2 hrs at 4°C. The protein content of the HDL was calculated using the absorbance at 280 nm and confirmed by Bradford protein assay. The purity of HDL fractions was confirmed by SDS-PAGE electrophoresis and immunoblot analysis using anti-apoA1 antibody (Abcam) and then stored at −80°C until use.
Anti apoa1 antibody
Manufactured by Abcam
The Anti-apoA1 antibody is a laboratory reagent used for the detection and quantification of apolipoprotein A-I (apoA-I) in biological samples. ApoA-I is a major structural and functional component of high-density lipoprotein (HDL) particles, which play a crucial role in cholesterol transport and metabolism. This antibody can be utilized in various analytical techniques, such as Western blotting, ELISA, and immunohistochemistry, to investigate the expression and distribution of apoA-I in research studies.
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Lab products found in correlation
2 protocols using anti apoa1 antibody
1
Alexa 594 Labeling of HDL
2
Fluorescent Labeling of HDL Particles
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The HDL (10 mg/ml) was dialyzed against borate buffer (pH 8.3) using 20 kDa cut-off dialysis cassette for 1 hr. The HDL was labeled by incubation with 25 μg of Alexa 594 NHS (10 mg/ml) overnight at room temperature with constant mixing. The free Alexa-594 dye was removed by dialysis using 20 kDa cut-off dialysis cassette against 50 mM HEPES buffer for 2 hrs at 4°C. The protein content of the HDL was calculated using the absorbance at 280 nm. The purity of HDL fractions was confirmed by SDS-PAGE electrophoresis (Yao et al., 2016 (link)) and immunoblot using an anti-apoA1 antibody (Abcam) and then stored at −80°C until use.
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