Log In Sign up
The largest database of trusted experimental protocols

Ephrin b1 biotin

Manufactured by R&D Systems
Visit Supplier

Ephrin-B1 Biotin is a recombinant protein product manufactured by R&D Systems. It consists of the extracellular domain of human Ephrin-B1 protein, tagged with a Biotin label. Ephrin-B1 is a member of the Ephrin family of proteins that function as ligands for Eph receptor tyrosine kinases.

Automatically generated - may contain errors

Lab products found in correlation

Efluor 780, by Thermo Fisher Scientific (2 mentions) Cd4 pe cy7, by Thermo Fisher Scientific (2 mentions) Active caspase 3, by Thermo Fisher Scientific (2 mentions) Igg1 fitc, by Thermo Fisher Scientific (2 mentions) Click it plus edu kit, by Thermo Fisher Scientific (2 mentions) Apc cy7, by R&D Systems (2 mentions) Cd138 bv421, by Thermo Fisher Scientific (2 mentions) B220 apc cy7 or bv785, by R&D Systems (2 mentions) Igd fitc, by R&D Systems (2 mentions) Gl 7 pacific blue, by R&D Systems (2 mentions) Fas pe cy7, by R&D Systems (2 mentions) Cd38 alexafluor647, by R&D Systems (2 mentions) Cd45.2 bv605, by R&D Systems (2 mentions) Hvem pe, by R&D Systems (2 mentions) Btla alexafluor 647 8f4, by R&D Systems (2 mentions) Cxcr4 biotin, by R&D Systems (2 mentions) Cd86 alexafluor647, by R&D Systems (2 mentions) Cd73 percp cy5, by Thermo Fisher Scientific (2 mentions) Tcrβ pacific blue, by Thermo Fisher Scientific (2 mentions) Cxcr5 bv605, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Ephrin-B1 (10 citations)

2 protocols using ephrin b1 biotin

1

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained on ice, in 96 round bottom plates in PBS supplemented with 2% FBS and 1mM EDTA. The following antibodies were used: B220 APC-CY7 or BV785 (clone RA3-6B2), IgD FITC (clone 11-26c.2a), GL-7 Pacific Blue (clone GL7), FAS PE-CY7 (clone Jo2), CD38 AlexaFluor647 (clone 90), CD45.1 PERCP-CY5.5, APC (clone A20), CD45.2 BV605, APC-CY7 (clone 104), HVEM PE (clone LH1), BTLA AlexaFluor 647 (8F4), Ephrin-B1 Biotin (polyclonal, R&D), CXCR4 Biotin (clone 2B11/CXCR4), CD86 AlexaFluor647 (clone GL-1), IgG1 FITC (clone RMG1-1), CD138 BV421 (clone 281-2), CD73 PERCP-CY5.5 (clone TY/11.8), CD4 PE-CY7 (clone GK1.5), TCRβ Pacific Blue (clone H57-597), CXCR5 BV605 (clone L138D7), PD-1 PE or FITC (clone 29F.1A12), CD40L PE (clone MR1), Vα2 PERCP-CY5.5 (clone B20.1), Active caspase-3 (clone C92-605), Fixable viability dye eFluor780 (eBioscience). NP-47-PE (Biosearch technologies). For intracellular staining, cells were fixed and permeabilized using BD Cytofix/Cytoperm kit. EdU was detected with Click-IT Plus EdU kit (Invitrogen). Data were collected on a BD LSRII and analyzed on FlowJo Software.
Mintz M.A., Felce J.H., Chou M.Y., Mayya V., Xu Y., Shui J.W., An J., Li Z., Marson A., Okada T., Ware C.F., Kronenberg M., Dustin M.L, & Cyster J.G. (2019). The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity, 51(2), 310-323.e7.
+ Open protocol
+ Expand
2

Multiparameter Flow Cytometry for Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained on ice, in 96 round bottom plates in PBS supplements with 2% FBS and 1mM EDTA. The following antibodies were used: B220 APC-CY7 or BV785 (clone RA3–6B2), IgD FITC (clone 11–26c.2a), GL-7 Pacific Blue (clone GL7), FAS PE-CY7 (clone Jo2), CD38 AlexaFluor647 (clone 90), CD45.1 PERCP-CY5.5, APC (clone A20), CD45.2 BV605, APC-CY7 (clone 104), HVEM PE (clone LH1), BTLA AlexaFluor 647 (8F4), Ephrin-B1 Biotin (polyclonal, R&D), CXCR4 Biotin (clone 2B11/CXCR4), CD86 AlexaFluor647 (clone GL-1), IgG1 FITC (clone RMG1–1), CD138 BV421 (clone 281–2), CD73 PERCP-CY5.5 (clone TY/11.8), CD4 PE-CY7 (clone GK1.5), TCRβ Pacific Blue (clone H57–597), CXCR5 BV605 (clone L138D7), PD-1 PE or FITC (clone 29F.1A12), CD40L PE (clone MR1), Vα2 PERCP-CY5.5 (clone B20.1), Active caspase-3 (clone C92–605), Fixable viability dye eFluor780 (eBioscience). NP-47-PE (Biosearch technologies). For intracellular staining, cells were fixed and permeabilized using BD Cytofix/Cytoperm kit. EdU detection was with Click-IT Plus EdU kit (Invitrogen). Data were collected on a BD LSRII and analyzed on FlowJo Software.
Mintz M.A., Felce J.H., Chou M.Y., Mayya V., Xu Y., Shui J.W., An J., Li Z., Marson A., Okada T., Ware C.F., Kronenberg M., Dustin M.L, & Cyster J.G. (2019). The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity, 51(2), 310-323.e7.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!