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Lasso catheter

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The Lasso catheter is a laboratory equipment used for electrophysiological mapping and ablation procedures. It features a circular mapping array with multiple electrodes to record cardiac electrical activity.

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10 protocols using lasso catheter

1

Numerical Simulation of Atrial Fibrillation

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We employed a numerical simulation to generate human atrial fibrillation data. A 10 cm × 10 cm 2D atrial tissue with a spatial resolution of 0.025 cm and sampling frequency of 500 Hz was simulated using the Nygren human atrial cell model [9 (link)]. A single stable rotor was initiated on the tissue and a 10-bipole Lasso catheter (Biosense Webster) with 15 mm diameter and 4.5-1-4.5 mm electrode spacing was simulated. The bipolar EGMs of the Lasso catheter were calculated from the unipolar EGMs which are the weighted sum of the Laplacian of the transmembrane potentials.
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2

Pulmonary Vein Isolation and Left Atrial Appendage Occlusion

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All operations were performed under local anesthesia. Before surgery, all patients (excluding patients with LA thrombosis) underwent TEE, and the LAAEV was recorded. Under the guidance of the CARTO 3 (Biosense Webster, USA) system, a Thermocool SmartTouch catheter (Biosense Webster, USA) was used to isolate the pulmonary veins, and a Lasso catheter (Biosense Webster, USA) was used to verify the bidirectional isolation of all pulmonary veins. No additional ablation lines were performed in this study. The sinus rhythm of all patients was recovered through ablation, medication, or electrical cardioversion. After ablation, blocking was conducted according to routine procedure. Watchman devices (Boston Scientific, Marlborough, Massachusetts, USA) were used as implanted occluders, and the PASS principle was followed to release the occluders.
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3

Pulmonary Vein Isolation Techniques

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Left atrial catheter ablation was performed using either radiofrequency ablation
or cryoballoon ablation at the discretion of the physician. For radiofrequency
ablation procedures, a 3.5-mm tip irrigated ablation catheter (Navistar
Thermocool, Biosense-Webster, Diamond Bar, California, USA) was used and placed
at the ostia of pulmonary vein (PV) to record PV potentials. For cryoablation
procedures, a 28-mm cryoballoon catheter (Arctic Front, Advance TM, Medtronic
Inc, Minneapolis, Minnesota, USA) was utilized to perform PVI. All patients
received circumferential ipsilateral PVI with guidance of electroanatomic
mapping (CARTO-3 system, Biosense-Webster, Diamond Bar, California, USA). The
endpoint of the PVI was the bidirectional conduction block from the atrium to
the PVs confirmed by Lasso catheter (Biosense-Webster, Diamond Bar, California,
USA).
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4

Simulating MPDC Electrograms and Rotational Activation

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The simulation was programmed to store the output needed to generate pseudo-ECGs (axial current) obtained from specific locations of the 2D tissue (Figure 1(b)). This feature was utilized for simulating an MPDC by providing the program with these location values determined according to the orientation of the electrodes of an MPDC. Using this method, a 20-pole (i.e., 10-bipole) MPDC (replication of Lasso catheter, Biosense Webster, Diamond Bar, CA) with a diameter of 15 mm was simulated and the pseudo-ECG values at those locations were obtained. A detailed description of these MPDC locations and their movement towards the RotA, which constitutes the objective of our simulation study, is provided in the section that discusses the simulation results. The activations of a 10-bipole simulated MPDC are shown in Figure 1(b), where the numbers 1 through 10 indicate the 10-bipole simulated electrodes.
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5

Anticoagulation and Catheter Ablation Protocol

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Patients were efficiently anticoagulated for more than 3 weeks. The antiarrhythmic drugs (AADs) were interrupted for ≥5 half-lives before catheter ablation. Thus, amiodarone was discontinued 3 weeks before the procedure. Electrophysiological studies and catheter ablation procedures were performed under general anesthesia using a 3D electroanatomical mapping (3D-EAM) system (CARTO 3, Biosense Webster, Diamond Bar, CA, USA, or EnSite Velocity, Abbott, St Paul, MN, USA) and a deflectable decapolar circular mapping catheter (Lasso catheter of variable diameter size (15–25 mm), interelectrode spacing 6 mm (Biosense Webster, Diamond Bar, CA, USA, or a spiral multipolar pulmonary vein catheter, Afocus II, diameter 20 mm, electrode spacing 5 mm, Abbott, St Paul, MN, USA). A transesophageal echocardiography was performed at the beginning of the procedure, to both exclude any thrombi in the left atrial appendage (LAA) and guide the transseptal puncture.
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6

Pulmonary Vein Isolation Guided by CARTO-3

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The CA procedure was guided by a CARTO-3 navigation system (Biosense Webster, Diamond Bar, CA, USA), which integrated the CT images and used a 3D mapping technique. Pulmonary vein isolation (PVI) was achieved with the bidirectional conduction block from the atrium to the PVs confirmed by a Lasso catheter (Biosense Webster, Diamond Bar, CA, USA). In cases in which AF did not end following PVI, additional linear lesions were carried out on the mitral isthmus and roof. The procedure was performed by the same surgical team, which had performed >1,000 cases of AF ablation.
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7

Characterizing Atrial Tissue from Clinical Electrograms

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This study includes nine patients recruited at Städtisches Klinikum Karlsruhe with the diagnosis of persistent AF. Patients were split into two groups; four patients were used to extract the clinical noise from the unipolar signals and train the machine learning algorithm. The other five patients were used as a proof of concept to test our approach to characterize the atrial tissue from clinical electrograms. Electroanatomical maps were acquired during sinus rhythm using the CARTO3 mapping system (Biosense Webster, Diamond Bar, CA, USA) with the Lasso catheter (Biosense Webster). The study was approved by the Institutional Review Board of Freiburg University in accordance with the Helsinki declaration. All patients gave written informed consent.
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8

Simulated Atrial Tissue Electrogram Modeling

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The proposed algorithm was tested on a 10 cm x 10 cm simulated 2D tissue sample with spatial resolution of 0.025 cm and a sampling frequency of 500 Hz using the Nygren et al. human atrial cell model [6 (link)]. The MPDC was simulated using a replica of a 20-unipole (10-bipole) Lasso catheter (Biosense Webster, Diamond Bar, CA) with 15 mm diameter and 4.5-1-4.5 mm electrode spacing (see Fig. 1A and B). The unipole electrogram for a specific tissue sample location is calculated by Eqn. (1):
ϕe(xr,yr)=k=1KΔvmk(xk-xr)2+(yk-yr)2 where K represents the total number of sample points on the tissue, Δ represents the Laplacian operator, vmk represents the simulated transmembrane potential at the kth atrial cell, (xk,yk) represents the coordinates of the kth atrial cell, and (xr,yr) represents the coordinates of the recording site. Bipole electrograms are calculated as the difference between MPDC unipole electrogram pairs. Fig. 1A and B show an example of bipole electrode locations and the corresponding bipole electrograms are shown in Fig. 1C.
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9

Pulmonary Vein Isolation Procedure

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All procedures were performed under general anaesthesia (Bruges and Luzern) or sedation (Graz and Leiden) and on direct OAC or uninterrupted vitamin K antagonists. Three centres (Bruges, Luzern, and Leiden) used ultrasound-guided femoral venous puncture, transoesophageal (or intra-cardiac) echocardiography, and oesophageal temperature monitoring. The CARTO-3, three-dimensional (3D) mapping system, and nMARQ RF generator (Biosense Webster, Diamond Bar, CA, USA) were used in all cases. Point-by-point PVI was performed as per the CLOSE protocol using the QDOT catheter in two modes (90 W over 4 s in temperature-controlled—Q MODE+ or 35/50 W in temperature and flow-controlled modes—Q MODE). Pulmonary vein isolation was confirmed with the lasso catheter (Biosense Webster) positioned in each circle, both at the end of the encirclement and during adenosine challenge.
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10

Pulmonary Vein Isolation Protocol

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According to the uniform standard of circumferential isolation of the pulmonary vein, CA was performed with a 3.5 mm tip irrigated ablation catheter (Navistar Thermocool, Biosense-Webster, Diamond Bar, CA, USA) placed at the ostia of pulmonary veins to record pulmonary vein potentials. The LA geometry reconstruction was guided by electroanatomic mapping (CARTO-3 system, Biosense-Webster, Diamond Bar, CA, USA). The bidirectional conduction block from the atrium to the pulmonary veins was the endpoint of the pulmonary vein isolation, as con rmed by Lasso catheter (Biosense-Webster, Diamond Bar, CA, USA). If AF was not terminated, additional linear ablation and complex fractionated atrial electrogram ablation were performed.
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