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Trfk5

Manufactured by BioXCell
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TRFK5 is a lab equipment product designed for cell culture applications. It is a programmable incubator that provides precise temperature, humidity, and gas control for optimal cell growth and maintenance. The TRFK5 offers consistent environmental conditions to support various cell types and experimental protocols.

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Lab products found in correlation

Tnp6a7, by BioXCell (2 mentions) Igg2b isotype control clone ltf 2, by BioXCell (1 mentions) Gemcitabine, by Sandoz (1 mentions) Anti gr1 antibody clone 1a8, by BioXCell (1 mentions) Anti ly6g 1a8, by BioXCell (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Sch58261, by Merck Group (1 mentions) Rmil 4, by BioLegend (1 mentions) Jes5 2a5, by BioXCell (1 mentions) Rmgm csf, by Merck Group (1 mentions) Rmil 33, by BioLegend (1 mentions) Mc 21, by BioXCell (1 mentions) Rat igg2a, by BioXCell (1 mentions)

Spelling variants of this product

Anti–IL-5 Ab (clone TRFK5) (2 citations) (clone TRFK5, (2 citations) α-IL-5 mAb (clone TRFK5; (2 citations)

7 protocols using trfk5

1

Modulation of Myeloma Progression by Cytokine Blockade

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αIL-5 (Clone TRFK5, BioxCell), or αIL-17A (Clone P59234.19, Amgen) and αIL-17R (Clone PL-31280, Amgen) or αIL-17A, αIL-17R and αIL-5, or isotype control (GL117, rat IgG2a) were injected i.p. (once a week for 9 weeks, 150 μg of each monoclonal antibody per mouse) in Early-MM Vk*MYC mice. Every three weeks mice were bled for M-spike quantification. Five days after the last injection mice were sacrificed and their BM assessed for the presence of Th17 cells and eosinophils. In experiments with the Vk12598 murine cell line, sex- and age-matched C57BL6J or IL-17KO mice were challenged i.v. with 1 × 106 Vk12598 cells, and C57BL6J mice were weekly injected i.p. with 100 μg per mouse of αIL-5 (Clone TRFK5, BioxCell), or αIL-17A (Clone P59234.19, Amgen) and αIL-17R (Clone PL-31280, Amgen), their combination, or isotype control (GL117, rat IgG2a) starting from the week of tumor challenge. Every week mice were bled for M-spike quantification.
Calcinotto A., Brevi A., Chesi M., Ferrarese R., Garcia Perez L., Grioni M., Kumar S., Garbitt V.M., Sharik M.E., Henderson K.J., Tonon G., Tomura M., Miwa Y., Esplugues E., Flavell R.A., Huber S., Canducci F., Rajkumar V.S., Bergsagel P.L, & Bellone M. (2018). Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression. Nature Communications, 9, 4832.
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2

Anti-IL-5 Treatment for Pericarditis

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To block IL-5 in vivo, mice were treated i.p. with 300 μg of anti-IL-5 monoclonal antibody (Clone: TRFK5, BioXCell) or isotype antibody (Clone: HRPN, BioXCell) every three days starting from a day before pericarditis induction.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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3

Gemcitabine and Immune Cell Depletion in Murine Breast Cancer

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A single dose of 60 mg/kg of gemcitabine (Sandoz, Boucherville, QC) was injected ip into mice bearing 17-day-old 4T1 tumors or 1 day following 4T1 primary tumor excision. gemcitabine was diluted to a 15 mg/ml working solution in physiological saline. Mice were euthanized and tissues were harvested 24, 48, 72, and 96 h post drug administration.
For immunological depletion of MDSCs, 100 μg of anti-Gr1 antibody (clone 1A8; BioXCell) or IgG2b isotype control (clone LTF-2; BioXCell) was administered via ip injection or intranasally every 4 days beginning 7 days after primary tumor implant. We found that 200 μg of anti-Gr1 antibody was lethal by the third injection. For immunological depletion of eosinophils, anti-IL-5 antibody (TRFK5, BioXCell) or isotype control (TNP6A7, BioXCell) was administered by weekly ip injection at 1 mg/kg in PBS.
Bosiljcic M., Cederberg R.A., Hamilton M.J., LePard N.E., Harbourne B.T., Collier J.L., Halvorsen E.C., Shi R., Franks S.E., Kim A.Y., Banáth J.P., Hamer M., Rossi F.M, & Bennewith K.L. (2019). Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs. Breast Cancer Research : BCR, 21, 103.
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4

Colitis Induction in Mice via Helicobacter and Anti-IL10R

Cited 1 time
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Colitis was induced in WT C57BL/6 mice by infecting with Hh (oral gavage) on 2 consecutive days with 5 × 107–2 × 108 CFU Hh and i.p. injection of 1 mg 1B1.2 (anti-IL10R) mAb on days 0 and 7 after Hh infection (Buonocore et al., 2010 (link)). Mice were killed 7 days after the last anti-IL10R mAb treatment (weeks 2–3). Where indicated, mice were i.p. injected two times per week with 0.4 mg of anti-GM-CSF (MP1-22E9; CSL Ltd) or isotype control (GL117, rat IgG2a) or 0.5 mg of anti-IL-5 (TRFK5; BioXCell), or three times per week with 0.25 mg anti-Ly6G (1A8; BioXCell) or isotype control (2A3, rat IgG2a) starting from the first day of Hh infection. Where indicated, mice were i.p. injected two times per week with sheep preimmune serum or sheep anti-Siglec-F serum. Where indicated, mice were i.p. injected daily with Resorcinol (1.25mg/kg) or PBS.
Griseri T., Arnold I.C., Pearson C., Krausgruber T., Schiering C., Franchini F., Schulthess J., McKenzie B.S., Crocker P.R, & Powrie F. (2015). Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis. Immunity, 43(1), 187-199.
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5

Murine Allergic Airway Inflammation Model

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Mice were anesthetized by isofluorane inhalation, followed by the intranasal administration of rmIL-33 (0.2 µg, Biolegend), rmIL-4 (0.5 µg), rmIL-13 (0.5 µg), rmGM-CSF (0.5 µg), rmIL-5 (0.5 µg), LPS (1 μg, Sigma), CpG (10 μg, Invivogen), Aspergillus protease allergen (0.01U, Sigma), Ragweed pollen extract (300 μg, Greer) or clodronate liposome (C.L.) (30% C.L./PBS, Liposoma B.V.) in 40 µl of PBS. Diphtheria toxin (10 ng/g, Sigma), IL-33R-Fc (10 mg/kg, AstraZeneca), anti-NK1.1 mAb (50 μg, PK136, BioXcell), anti-CCR2 (20 μg, MC-21, provided by Prof. Matthias Mack52 (link)), anti-CCL2 (200 μg, MCP-1, BioXcell), anti-Ly6C/G (200 μg, GR-1, BioXcell), anti-Ly-6G (200 μg, 1A8, BioXcell), anti-CD4 (100 μg, GK1.5, BioXcell), anti-IL-10 (300 μg, JES5-2A5, BioXcell), anti-TGF-β (400 μg, 1D11.16.8, BioXcell), anti-IL-5 (100 μg, TRFK5, BioXcell), rat IgG1, κ (BioXcell), rat IgG2a (BioXcell), or rmIL-33 (0.5 µg, Biolegend) was administered by intraperitoneal injection in 100 µl of PBS. A2AR antagonist (20 μg, SCH 58261, Sigma) was administered in DMSO/PBS (v/v).
Schuijs M.J., Png S., Richard A.C., Tsyben A., Hamm G., Stockis J., Garcia C., Pinaud S., Nicholls A., Ros X.R., Su J., Eldridge M.D., Riedel A., Serrao E.M., Rodewald H.R., Mack M., Shields J.D., Cohen E.S., McKenzie A.N., Goodwin R.J., Brindle K.M., Marioni J.C, & Halim T.Y. (2020). ILC2-driven innate immune checkpoint mechanism antagonizes NK cell anti-metastatic function in the lung. Nature immunology, 21(9), 998-1009.
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6

Anti-IL-5 Treatment for Pericarditis

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To block IL-5 in vivo, mice were treated i.p. with 300 μg of anti-IL-5 monoclonal antibody (Clone: TRFK5, BioXCell) or isotype antibody (Clone: HRPN, BioXCell) every three days starting from a day before pericarditis induction.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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7

Anti-IL-5 Antibody Treatment in Neonatal Mice

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Mice were treated by subcutaneous injections of 100 µg of either anti-IL-5 antibody (TRFK5, BioXCell, NH, USA) or an isotype control antibody (TNP6A7, BioXCell, NH, USA) at the age of 3, 6, 9 and 12 days after birth. At the age of 14 days, mice were killed by overdose of pentobarbital, BAL fluid was collected and lungs were inflated and fixed in formalin for assessment of structural lung damage as described below.
Brown R., Paulsen M., Schmidt S., Schatterny J., Frank A., Hirtz S., Delaney R., Doherty D., Hagner M., Taggart C., Weldon S, & Mall M.A. (2020). Lack of IL-1 Receptor Signaling Reduces Spontaneous Airway Eosinophilia in Juvenile Mice with Muco-Obstructive Lung Disease. American journal of respiratory cell and molecular biology, 62(3).
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