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NA12882 is a cell line derived from a female of northern European descent. It serves as a reference sample for genetic studies and analysis. The core function of NA12882 is to provide a standardized genetic profile for comparison and validation purposes in research and clinical applications.

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Lab products found in correlation

Gentra puregene cell kit, by Qiagen (2 mentions) Na12877, by Coriell Institute for Medical Research (2 mentions) Gm20847, by Coriell Institute for Medical Research (2 mentions) Gm12878, by Coriell Institute for Medical Research (2 mentions) Nci h2228, by American Type Culture Collection (2 mentions) E z n a sq dna rna protein kit, by Omega Bio-Tek (2 mentions) Bluepippin, by Sage Science (2 mentions)

2 protocols using na12882

1

Genomic DNA Extraction and Size Selection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Institutional Review Board (IRB) at Stanford University School of Medicine approved the study. Informed consent was obtained and the samples were made available from the Stanford Cancer Center Tissue Bank. This study used a primary colorectal adenocarcinoma and matched normal tissue that were collected at time of surgical resection and flash frozen. Both samples had genomic DNA extracted with the E.Z.N.A. SQ DNA/RNA Protein Kit (Omega Bio-Tek). The genomic DNA did not require further size selection or processing. We quantified the DNA with Life Technologies Qubit.
For the commercially acquired genomic DNA, we size selected DNA molecules 20 kb or higher using the BluePippin (Sage Science) (NA12877 and NA12882 from Coriell, and NCI-H2228 from ATCC). In addition, we harvested immortalized human lymphocyte cells (GM12878 and GM20847 from Coriell) and genomic DNA was extracted using the Gentra Puregene Cell kit (Qiagen).
Zheng G.X., Lau B.T., Schnall-Levin M., Jarosz M., Bell J.M., Hindson C.M., Kyriazopoulou-Panagiotopoulou S., Masquelier D.A., Merrill L., Terry J.M., Mudivarti P.A., Wyatt P.W., Bharadwaj R., Makarewicz A.J., Li Y., Belgrader P., Price A.D., Lowe A.J., Marks P., Vurens G.M., Hardenbol P., Montesclaros L., Luo M., Greenfield L., Wong A., Birch D.E., Short S.W., Bjornson K.P., Patel P., Hopmans E.S., Wood C., Kaur S., Lockwood G.K., Stafford D., Delaney J.P., Wu I., Ordonez H.S., Grimes S.M., Greer S., Lee J.Y., Belhocine K., Giorda K.M., Heaton W.H., McDermott G.P., Bent Z.W., Meschi F., Kondov N.O., Wilson R., Bernate J.A., Gauby S., Kindwall A., Bermejo C., Fehr A.N., Chan A., Saxonov S., Ness K.D., Hindson B.J, & Ji H.P. (2016). Haplotyping germline and cancer genomes using high-throughput linked-read sequencing. Nature biotechnology, 34(3), 303-311.
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2

Genomic DNA Extraction and Size Selection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Institutional Review Board (IRB) at Stanford University School of Medicine approved the study. Informed consent was obtained and the samples were made available from the Stanford Cancer Center Tissue Bank. This study used a primary colorectal adenocarcinoma and matched normal tissue that were collected at time of surgical resection and flash frozen. Both samples had genomic DNA extracted with the E.Z.N.A. SQ DNA/RNA Protein Kit (Omega Bio-Tek). The genomic DNA did not require further size selection or processing. We quantified the DNA with Life Technologies Qubit.
For the commercially acquired genomic DNA, we size selected DNA molecules 20 kb or higher using the BluePippin (Sage Science) (NA12877 and NA12882 from Coriell, and NCI-H2228 from ATCC). In addition, we harvested immortalized human lymphocyte cells (GM12878 and GM20847 from Coriell) and genomic DNA was extracted using the Gentra Puregene Cell kit (Qiagen).
Zheng G.X., Lau B.T., Schnall-Levin M., Jarosz M., Bell J.M., Hindson C.M., Kyriazopoulou-Panagiotopoulou S., Masquelier D.A., Merrill L., Terry J.M., Mudivarti P.A., Wyatt P.W., Bharadwaj R., Makarewicz A.J., Li Y., Belgrader P., Price A.D., Lowe A.J., Marks P., Vurens G.M., Hardenbol P., Montesclaros L., Luo M., Greenfield L., Wong A., Birch D.E., Short S.W., Bjornson K.P., Patel P., Hopmans E.S., Wood C., Kaur S., Lockwood G.K., Stafford D., Delaney J.P., Wu I., Ordonez H.S., Grimes S.M., Greer S., Lee J.Y., Belhocine K., Giorda K.M., Heaton W.H., McDermott G.P., Bent Z.W., Meschi F., Kondov N.O., Wilson R., Bernate J.A., Gauby S., Kindwall A., Bermejo C., Fehr A.N., Chan A., Saxonov S., Ness K.D., Hindson B.J, & Ji H.P. (2016). Haplotyping germline and cancer genomes using high-throughput linked-read sequencing. Nature biotechnology, 34(3), 303-311.
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