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Rat igg total elisa kit

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The Rat IgG Total ELISA Kit is a quantitative immunoassay designed to measure the total immunoglobulin G (IgG) levels in rat samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

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Lab products found in correlation

Duoflow, by Thermo Fisher Scientific (2 mentions) Lsm 800, by Zeiss (2 mentions) Cm1860, by Leica (2 mentions) Transwell system, by Corning (2 mentions) Ultraviolet visible spectrophotometer, by PerkinElmer (1 mentions) Agilent 1200 infinity series, by Agilent Technologies (1 mentions) Anti il 6ralpha clone 15a7, by BioXCell (1 mentions)

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Rat ELISA kits (40 citations) ELISAs (35 citations) Rat IgG ELISA kit (2 citations)

6 protocols using rat igg total elisa kit

1

Drug Release Evaluation in PBS

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Drug release tests were carried out at 37°C in phosphate-buffered saline (PBS) under stirring. The released DOX was analyzed with an ultraviolet-visible spectrophotometer (PerkinElmer). The released OXA was measured by inductively coupled plasma atomic emission spectroscopy (Thermo Fisher Scientific). The released R837 was detected by high-performance liquid chromatography (Agilent 1200 Infinity Series), and the released anti-PDL1 was determined with the Rat IgG Total ELISA Kit (eBioscience).
Chao Y., Liang C., Tao H., Du Y., Wu D., Dong Z., Jin Q., Chen G., Xu J., Xiao Z., Chen Q., Wang C., Chen J, & Liu Z. (2020). Localized cocktail chemoimmunotherapy after in situ gelation to trigger robust systemic antitumor immune responses. Science Advances, 6(10), eaaz4204.
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2

Nanomedicine-based Cancer Immunotherapy

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Sora and HMSNs were poured into PBS and stirred for 24 h. Then the mixture was centrifuged and the precipitate was collected for drying. The concentration of Sora in the supernatant was determined by UV-VIS method, and the drug loading of Sora was calculated.
For aPD-1 conjugation, PLTM extracted from 2 mL blood was first re-suspended in PBS (pH = 8, + PGE1, 1 µM) and incubated with 0.1 mg/mL Trauts reagent at room temperature for 30 min; then excessive Trauts reagent was removed by centrifugation and washed 3 times with PBS buffer (+ PGE1, 1 µM); finally, PLTM-HMSNs@Sora and aPD-1 were mixed in PBS buffer (+ PGE1, 1µM) and aPD-1-PLTM-HMSNs@Sora was obtained after centrifugation for 10 min. The amount of aPD-1 conjugated to the platelets was measured via ELISA (Rat IgG Total ELISA Kit; eBioscience). After labelling aPD-1 with CY5.5 and PLTM-HMSNs@Sora with FITC, the conjugation of aPD-1 with PLTM-HMSNs@Sora was verified by confocal microscopy.
Da X., Cao B., Mo J., Xiang Y., Hu H., Qiu C., Zhang C., Lv B., Zhang H., He C, & Yang Y. (2024). Inhibition of growth of hepatocellular carcinoma by co-delivery of anti-PD-1 antibody and sorafenib using biomimetic nano-platelets. BMC Cancer, 24, 273.
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3

Measuring Rat Antibody Levels in Mice

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B6 mice received intraperitoneal injections with endotoxin-free antibodies purchased from BioXcell including: anti-IL-6Ralpha (clone 15A7) and rat isotype control (clone LTF-2). Mice were sacrificed at defined time points and peripheral blood was collected via cardiac puncture. Blood was spun at 13,000×g for 15 minutes and serum was collected for analysis. Presence of rat antibodies within the serum was measured using a Rat IgG total ELISA kit (EBioscience, cat no. 88-50490) according to manufacturer recommendations.
Long K.B., Tooker G., Tooker E., Luque S.L., Lee J.W., Pan X, & Beatty G.L. (2017). IL-6 receptor blockade enhances chemotherapy efficacy in pancreatic ductal adenocarcinoma. Molecular cancer therapeutics, 16(9), 1898-1908.
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4

Evaluating GEM and Antibody Release

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Release studies were performed at 37°C with constant agitation in PBS. H 2 O 2 (Sigma) was added to samples to study the GEM and antibody release. The released GEM was analyzed by HPLC, and the antibody release was determined by the Rat IgG Total ELISA Kit (eBioscience).
Wang C., Wang J., Zhang X., Yu S., Wen D., Hu Q., Ye Y., Bomba H., Hu X., Liu Z., Dotti G, & Gu Z. (2018). In situ formed reactive oxygen species-responsive scaffold with gemcitabine and checkpoint inhibitor for combination therapy. Science translational medicine, 10(429).
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5

Controlled Drug Release from AVB Microcapsules

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The drugs were loaded into AVB in the second mixing process. The drug release behavior was measured using a Transwell system (Corning, catalog no. 3422) in PBS at 37°C to mimic the physiological environment. The AVB (50 μl, 1 wt %) encapsulated with Mel (0.3 mg) and rat IgG (0.2 mg, the substitution of αPDL1) was loaded into the upper chamber, and the released Mel and IgG were collected from the bottom of the chamber at different time points, which were measured using HPLC (BioLogic DuoFlow) and the rat IgG total ELISA kit (Thermo Fisher Scientific, catalog no. 88-50490-88) separately. In addition, AVB-encapsulated RhoB- and Cy5.5-labeled rat IgG (IgG-Cy5.5) at different time points were frozen in optimal cutting temperature medium at −80°C and then cut into slices (thickness = 15 μm) using a cryotome (Lecia CM1860) at −25°C and were sealed and imaged by a confocal microscope (Zeiss LSM 800).
Chen M., Hu J., Gao H., Shen J., Wei T., Yao J., Zhang Y., Gu P., Liu Z, & Chen Q. (2023). An immunotherapeutic artificial vitreous body hydrogel to control choroidal melanoma and preserve vision after vitrectomy. Science Advances, 9(44), eadh1582.
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6

Controlled Drug Release from AVB Microcapsules

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The drugs were loaded into AVB in the second mixing process. The drug release behavior was measured using a Transwell system (Corning, catalog no. 3422) in PBS at 37°C to mimic the physiological environment. The AVB (50 μl, 1 wt %) encapsulated with Mel (0.3 mg) and rat IgG (0.2 mg, the substitution of αPDL1) was loaded into the upper chamber, and the released Mel and IgG were collected from the bottom of the chamber at different time points, which were measured using HPLC (BioLogic DuoFlow) and the rat IgG total ELISA kit (Thermo Fisher Scientific, catalog no. 88-50490-88) separately. In addition, AVB-encapsulated RhoB- and Cy5.5-labeled rat IgG (IgG-Cy5.5) at different time points were frozen in optimal cutting temperature medium at −80°C and then cut into slices (thickness = 15 μm) using a cryotome (Lecia CM1860) at −25°C and were sealed and imaged by a confocal microscope (Zeiss LSM 800).
Chen M., Hu J., Gao H., Shen J., Wei T., Yao J., Zhang Y., Gu P., Liu Z, & Chen Q. (2023). An immunotherapeutic artificial vitreous body hydrogel to control choroidal melanoma and preserve vision after vitrectomy. Science Advances, 9(44), eadh1582.
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