Mouse immunoglobulin g igg

Antibodies were obtained from the following sources: antiserum for P. gingivalis whole cells was kindly donated by Dr. Fuminobu Yoshimura (Aichi-gakuin University, Aichi, Japan); mouse monoclonal antibody specific for ICAM-1, goat polyclonal antibody specific for ICAM-1, mouse monoclonal antibody specific for TNFRI, mouse monoclonal antibody specific for TNFRII and mouse immunoglobulin G (IgG) (R & D Systems, Minneapolis, MN); mouse monoclonal antibody specific for Rab5 (BD Biosciences); rabbit polyclonal antibody specific for ICAM-1 (Santa Cruz Biotechnology, Dallas, TX); goat IgG (Alpha Diagnostic Intl. Inc., San Antonio, TX); mouse monoclonal antibody specific for β-actin (Biovision Inc., Milpitas, CA); anti-rabbit IgG-Alexa 555 and anti-rabbit IgG-Alexa 633 (Invitrogen); mouse monoclonal antibody specific for GFP (Novus Biologicals, Littleton, CO), anti-mouse IgG-HRP, anti-rabbit IgG-HRP and mouse monoclonal antibody specific for β-actin (Cell Signaling Technology, Danvers, MA).

Tissue culture- and fibronectin-coated plates were purchased from Corning (Corning, NY). Recombinant human (rh)FGF-2, rhIL-6, rhIL-8 and rhTGFβ1 and mouse blocking monoclonal antibody to CXCL1/KC (IL-8) and mouse immunoglobulin G (IgG) were purchased from R&D Systems (Minneapolis, MN). H₂O₂, carbonyl-cyanide m-chlorophenylhydrazzone (CCCP) and Fulvestrant (ICI 182780) were purchased from Sigma-Aldrich (Saint Louis, MO). Radioimmunoprecipitation assay (RIPA) buffer was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit monoclonal antibodies to human E-Cadherin (clone 24E10), N-Cadherin (clone D4R1H) XP), Slug (clone C19G7), β-Catenin (clone D10A8) were purchased from Cell Signaling (Danvers, MA). Mouse monoclonal antibody to estrogen receptor α (ERα) (clone F-10), N-cadherin (clone H-4), horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and goat anti-mouse IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Vybrant CM-DiI fluorescent tracking dye was purchased from Invitrogen-Molecular Probes (Carlsbad, CA).

Recombinant murine TRAIL was purchased from PeproTech (Rocky Hill, NJ) and diluted with phosphate-buffered saline (PBS) to produce a dose of 1.5 μg/kg body weight (BW)/rat. The soluble TNF-α receptor, etanercept (Enbrel), was purchased from Pfizer (New York City, NY) and diluted with PBS to produce a dose of 6 mg/kg BW/rat. Mouse immunoglobulin G (IgG) was purchased from R&D Systems (Minneapolis, MN) and diluted with PBS to produce a dose of 200 ng/rat. Rats were injected subcutaneously at the hind paws with PBS, IgG, TRAIL, or etanercept starting on day 4 after the booster immunization (day 11 after the bCII immunization).